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Impact of postures on Cobb angle measurements in scoliosis mouse models

posted on 2020-08-13, 04:52 authored by Zheyi Chen
This data set concludes all the images, charts, statistics and reference that occurred in my thesis. Here is the abstract.
Scoliosis is defined by a Cobb angle measurement that exceeds 10° from the anterior-posterior standing radiograph. Its diagnosis and management decisions rely on the stringent assessment of the curve magnitude.

There are quadrupedal animal models like pigs, rabbits and rats, bipedal animal models like chickens and non-human primates. There are also guppy and zebrafish which are widely used in genetic model establishment. Mouse is suitable for either - the mechanical model or the genetic model, and the genetic background is clear with a relatively high gene homology, which makes it a promising animal model for future scoliosis study.

In humans, the effect of positioning error on Cobb angle measurements and the measurement error expected from variations in imaging have been investigated intensively. As there is no study that describes these two kinds of error in animal models, the study objective is to test the effect of postures on the angle measurement as well as to establish a standard for mouse imaging.

In present study, we first collected 77 mice to do radiography. During the experiment, we described the criteria for a standard right posture in terms of the symmetry of body. We found out that the incidence of scoliosis in mouse population was around 28.6%. There was a big variation in curve magnitude as well. We took weekly X-rays for 24 mice and found out that the variation of multiple-time measurement was also very big. In the second part of the study, we collected 82 mice and designed 14 postures that may vary during imaging. The results showed that tilting of the head (>30°) and the imbalance of the pelvis would greatly affect the curve magnitude with effect size (Glass’s delta) over 1.50 which is huge according to the definition.
Our work exemplified the importance of standard protocol during imaging when using animal model in the scoliosis study. This is important for all imaging studies in these animals. We recommend utilizing this standard in studying various disorders of the spine.


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