Supporting Data for thesis entitled "Single Molecule Analysis of hKIF27 shows its processive movements with long binding duration along ciliary microtubules"

Kinesin motor proteins play important roles in intracellular transport, ciliary function and microtubule dynamics regulations. KIF27 is a kinesin motor protein from Kinesin-4 family and homologues to the mammalian KIF7. KIF27 knock-out mice exhibit hydrocephalus and nasal mucus obstruction, indicating cellular defects associated with motile cilia. To gain insight into its roles in regulating motile biogenesis and function, we set out to characterize the modes of actions for KIF27 using recombinant proteins and TIRF microscopy.