Supporting data for "Behavioral and mechanistic characterization of addictive properties of the classic anesthetic nitrous oxide and the anti-addictive effects of the natural compound cryptotanshinone"

This research is focused on the mechanisms under the addictive properties of Nitrous oxide. In this research, we used behavioral test (Conditioned place preference), molecular biological technique (PCR, Western blot, flow), imaging methods (IF and rs-fMRI) to explore the mechanism. Using these methods, the rewarding effects and molecular changes of in nitrous oxide treating mice and rats.