Supporting data for "Bioengineering of the human endometrial stem cell niche: A regulatory environment for endometrial regeneration"

Endometrium is the mucosal lining of the uterus where embryo implantation occurs. Human endometrial thickness of less than 7 mm significantly reduces the likelihood of a successful pregnancy. Endometrial mesenchymal stem cells (eMSC) located near blood vessels in the endometrium possess great translational therapeutic potential as a cell source for promoting the endometrium regeneration in patients with thin endometrium. Yet, a lack of comprehensive understanding of the regulatory mechanisms of eMSC hinders the development of effective strategies to optimize the in vitro expansion and maximize the in vivo therapeutic effects.

The stem cell niche refers to the microenvironment controlling stem cell behavior. This thesis focuses on identifying key regulatory niche factors to optimize the expansion and therapeutic application of eMSC. Research included in this study explores the effects of niche factors such as endothelial cells, oxygen level, and extracellular matrix (ECM) on eMSC. Additionally, two biomaterials, EndoMatriGel and EndoMatriScaffold, were developed and characterized as platforms for delivering eMSC to enhance their function.

The findings in this thesis significantly advanced the knowledge base for cell therapy for endometrium regeneration by providing insights for tailoring optimal culture substrates for eMSC. The development of novel biomaterials as delivery systems has great potential for enhancing the therapeutic effects of eMSC in endometrial regeneration.