Supporting data for Biological importance of TSPYL1 as a transcription regulator

To confirmed EMT phenotypes caused by TSPYL1 deficiency, the EMT functional study including scratch assay, tranwell assay and transwell with Matrigel assay were conducted in TSPYL1 knockdown A549 cells. To decipher the mechanism of TSPYL1 regulated EMT, the target genes of TSPYL1 were screened with CUT&RUN-seq. To study the importance of TSPYL1 in vivo, the lung sections H&E stanning from WT and Tspyl1 KO mice were analyzed.