Supporting data for "Characterizing the role of the STK36/ULK4 complex in motile ciliogenesis"

Serine/threonine kinase 36 (STK36), when mutated, causes primary ciliary dyskinesia (PCD), a genetic condition with multi-system manifestations characterized by defects in motile cilia structure and function. The ortholog of STK36 in Drosophila, Fused, is required for Hedgehog (Hh) signal transduction. STK36 and Unc-51-like kinase 4 (ULK4), both from the Unc-51-like kinase (ULK) family, form a complex, which is an ancestral, conserved regulator of the assembly and function of motile cilia that can be found in organisms ranging from flagellated protists to mammals. However, the molecular mechanism of how the complex modulates motile ciliogenesis remains unknown. The data here, including immunoprecipitation mass spectrometry analysis, protein complex structure prediction, in vitro kinase assays, cell-based protein-protein interaction assays, and confocal microscopy images, contribute to the understanding of this mechanism.