Supporting data for ‘Chemically modified collagen and associated naturally occurring biomaterials and their potential applications in tissue engineering’

This dataset is the supporting data for ‘Chemically modified collagen and associated naturally occurring biomaterials and their potential applications in tissue engineering’. In this thesis, we aimed to fabricate a set of novel materials and verified their potential applications in tissue engineering. This dataset includes the characterization of the chemical, biological and mechanical properties of the natural materials-based biomaterials. 

This dataset contains raw data collected in the tissue engineering laboratory and the facility center such as the electron microscopy unit (EMU) and core facility at HKU, and the quantitative and qualitative data were analyzed and processed by the open-access software, such as Image J, SPSS, and Origin. This dataset includes three parts: fold 1 IVD, folder 2 MSCs differentiation, and folder 3 gene transfection. Supporting data folder 1 IVD contains data on the fabrication of the GAG-rich scaffolds for the nucleus pulposus and their potential applications in NP tissue engineering. This folder includes the FTIR, GAG content, Zeta potential, SEM, TEM, fluorescence images, histological images, PCR, elastic modulus, and the disc height recovery results, demonstrating the successful fabrication of the GAG-rich scaffolds and the good biological, and functional ability of these GAG-rich scaffolds. Supporting data folder 2 MSCs differentiation contains data on the multiple differentiation abilities of hMSCs, which includes the gross appearance, histological staining, ultrastructure, and PCR data, verifying the concept that biomimetic scaffolds benefit the hMSCs differentiation towards specific lineage. Supporting data folder 3 gene transfection contains data on the gene transfection of chemically modified collagen and Chitosan, including the FTIR, zeta potential, ultrastructure, cellular uptake, and gene transfection efficacy of the modified collagen, demonstrating that the modified collagen is a candidate for gene transfection and protein secretion.