Supporting data for"Cholesterol supply from PI-Activated TREM2+ Macrophages to Tregs Drives Lymph Node Immunosuppressive Pre-Metastatic Niche in Esophageal Squamous Cell Carcinoma"

<p dir="ltr">This dataset was designed to investigate the mechanisms by which PI-activated TREM2⁺ macrophages establish an immunosuppressive pre-metastatic microenvironment within esophageal squamous cell carcinoma (ESCC) .</p><p dir="ltr">lymph nodesImmunofluorescence imaging was used to determine the spatial co-localization of TREM2⁺ macrophages with Tregs and tumor cells in mouse and human lymph nodes. Multi-marker images (e.g., CD68, PANK, TREM2, CD3, CD20) helped delineate the immune cell composition and its anatomical location within the lymph nodes. Flow cytometry was widely used to assess myeloid and T cell subsets in metastatic lymph nodes and IL8-induced changes in spleen and bone marrow mononuclear cells). We used chip-based arrays to assess cytokine signatures and compared the cytokine profiles of tumor lymph nodes with those of control lymph nodes. These data support changes in the pre-metastatic microenvironment. We used chip-based arrays to assess cytokine signatures and compared the cytokine profiles of tumor lymph nodes with those of control lymph nodes. These data support changes in the pre-metastatic microenvironment.Additionally, we performed IVIS imaging to track tumor cell dissemination and evaluate therapeutic interventions.</p><p dir="ltr">Overall, this dataset provides a data foundation for dissecting the metabolic and immune crosstalk between PI-activated TREM2⁺ macrophages and regulatory T cells, and how this axis orchestrates the formation of a pre-metastatic immunosuppressive microenvironment in ESCC draining lymph nodes.</p>