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Supporting data for "Development of Nucleic Acid-Based Tools for Pathogen Diagnostics and Real-Time RNA Imaging Using Droplet-based Microfluidics"
The research is on developing a point-of-care system for detecting pathogenic nucleic acids using digital droplet PCR or LAMP amplification techniques. This was possible using PDMS chips integrated with a heating system controlled by Arduino. The second part of the project is on high-throughput screening of RNA at-tamers that bind to a specific ligand with high fluorescence intensity. Millions of random DNA sequences were screened using fluorescence activated droplet sorting (FADS) and the best at-tamers were selected and screened.