Supporting data for “Development of multifunctional fibroblast membrane nanoplatforms for tumor-targeted drug delivery and immune activation”
<p dir="ltr">This dataset contains all original experimental data supporting the findings presented in Chapter 3 and Chapter 4 of my PhD thesis. The overall project focuses on the development of fibroblast membrane-coated nanoparticles for tumor-targeted therapy via ferroptosis induction (Chapter 3) and immunomodulation through STING pathway activation (Chapter 4). All experiments were conducted using standard in vitro assays and multiple murine tumor models, and results were recorded using a combination of imaging, molecular analysis, and functional evaluations.</p><p dir="ltr">The uploaded dataset is organized into two main folders—Dataset (Chapter 3) and Dataset (Chapter 4)—each containing several subfolders corresponding to major experimental categories:</p><p dir="ltr"><b>Dataset (Chapter 3)</b> includes five subfolders:</p><ul><li><b>Function characterization</b>: TEM images, immunofluorescence images, cellular uptake and transwell migration assays (TIF format), and CSV files of nanoparticle size/zeta potential analysis using GraphPad.</li><li><b>Targeting evaluation</b>: In vitro and in vivo targeting data, including tumor spheroid uptake images and IVIS fluorescence imaging (TIF format).</li><li><b>Photothermal evaluation</b>: Thermal images (PNG/JPG) and CSV data from UV-vis spectrum analysis.</li><li><b>In vitro therapeutic efficacy</b>: Confocal microscopy images of FerroOrange staining (TIF format), and CSV files of cell viability, ELISA, BODIPY staining, and GSH analysis.</li><li><b>In vivo therapeutic efficacy</b>: H&E and immunohistochemistry images (TIF/JPG), and CSV data of mouse body weight, tumor weight, and tumor MDA levels.</li></ul><p dir="ltr"><b>Dataset (Chapter 4)</b> includes three subfolders:</p><ul><li><b>Function characterization</b>: Cellular uptake images (TIF) and western blot data (PDF).</li><li><b>Targeting evaluation</b>: Tumor spheroid uptake images (TIF), and fluorescence/immunofluorescence imaging results (PDF).</li><li><b>In vivo therapeutic efficacy</b>: Therapeutic outcomes and survival curves (PDF), along with CSV files of body weight, tumor volume, tumor weight, and flow cytometry data.</li></ul><p dir="ltr">All image formats (TIF, JPG, PNG, PDF) are directly readable and organized by experiment type. CSV files include raw and processed numerical data analyzed primarily using GraphPad Prism. No sensitive or personally identifiable information is included.</p><p dir="ltr">This dataset provides a comprehensive and reproducible record of the experimental work conducted during my doctoral studies.</p>
