Supporting data for "Dissecting the molecular determinants of the major multidrug efflux pump transporter MexB towards its antibiotic and physiological substrates: the extensively drug-resistant genotype PA154197 as a paradigm"

This dataset includes both the raw data and processed data resulting from analysis for my thesis titled with " Dissecting the molecular determinants of the major multidrug efflux pump transporter MexB towards its antibiotic and physiological substrates: the extensively drug-resistant genotype PA154197 as a paradigm ".

PA154197 is a clinical strain isolated from a bloodstream infection at the Queen Mary Hospital in Hong Kong. To investigate the role of the RND efflux pump MexAB-OprM in cell physiology, I mainly conducted the measurement of virulence factors (PYO, proteolytic activities), in-vitro gene expression studies (RT-qPCR), in-vivo gene expression studies (promoter-lux reporter), the quantification of auto-inducer signals based on LC-MS/MS (3OC12-HSL, C4-HSL) or biosensor (AHQ). To study the MexB recognition determinants towards its substrates, a mexB mutant library was constructed using the native CRISPR-Cas system. Minimum inhibitory concentrations to different groups of antibiotics of these mutants were measured. The molecular docking analysis based on Autodock Vina was conducted to predict the possible ligand binding poses inside the distal binding pocket.