The current study demonstrated that HUVECs and TGF-β1 could induce DPSC differentiation into functional SMCs, and Ang1/Tie2/VE-cadherin and VEGF/VEGFR2 signaling pathways contributed to vascular stabilization induced by DPSCs. This study broadens our understanding of the angiogenic potential of DPSCs, which may open a new avenue for the application of DPSCs in vascular tissue engineering for dental pulp regeneration.