Contain clean data from methylRAD sequencing for quality control, methyl level distribution, genome mapping, methylation expression, sites level annotation, pearson correlation, gene depth statistics, differentially methylated sites and genes, the function of differentially methylated genes, supplementary materials which include bioinformatic flow chart, enrichment formula and score, illumine quality, as well as the technicality process for methylRAD enzymes including FspEI and LpnPI.