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Supporting data for "Exploring how systemic inflammation affects the neurotoxic accumulation of α-synuclein" Part 2
This dataset explores the neurotoxic effect inflammation and oxidative damage has on the accumulated, phoshorylated alpha-synuclein induced by recombinant preformed fibrils. Recombinant preformed fibrils of alpha-synuclein effectively induce phosphorylation and accumulation of alpha-synuclein into Lewy Body-like inclusions, but exactly how these inclusions lead to neuronal death remains elusive. To examine early stress responses, the phoshorylation of tau and the formation of stress granules were examined in vitro by confocal imaging. The dataset reveals a relationship between phosphorylated, aggregated alpha-synuclein and the formation of stress granules. To examine how oxidative damage or inflammation affects the neurotoxity of phoshorylated alpha-synuclein induced by recombinant preformed fibrils of alpha-synuclein in vivo, the hippocampal neuronal density, tau phoshorylation, oxidative damage and inflammation was evaluated using confocal imaging. To further examine the functional role microglia have in this modulation, microglial phagocytosis was evaluated using confocal imaging of cultured primary microglia. The study reveals
a relationship between phosphorylated, aggregated alpha-synuclein and the formation of stress granules, where inflammation regulates the neurotoxic outcome in vivo.