This is the raw data to support the thesis titled "Harnessing multiphasic microdroplets (MPMD) towards nucleic acid engineering". The raw data are arranged according to the content or date of experiment. The data includes fluorescence intensity recorded during droplet sorting in microfluidics, both for the selection of Broccoli aptamer and the selection of Corn aptamer. The content also contains phase diagram of RNA and poly-lysine coacervation, together with the free energy calculated based on the Overbeek-Voorn model.