Supporting data for “Identification and characterization of pharmacologically active small molecules modulating spheroid attachment and embryo implantation”

Infertility affects millions of couples worldwide, with embryo implantation failure being one of the biggest challenges. This research aims to identify and characterize small molecules that can enhance endometrial receptivity and embryo implantation. A multi-step approach was used, including high-throughput screening of 1280 small molecules from the Library of Pharmacologically Active Compounds (LOPAC), bioinformatics analysis, and in vitro co-culture assays with non-receptive endometrial cells to identify promising compounds. RNA sequencing and quantitative PCR were applied to assess differential gene expression, focusing on pathways relevant to implantation. In vivo validation was conducted using a transcervical delivery system in a mouse model, with implantation sites marked by Chicago blue dye at specific time points to precisely evaluate implantation outcomes​​​.