Firstly, this dataset shows the inhibitory effect of different kinds of hydrocolloids on the formation of heterocyclic amines in both chemical model systems and roast beef; Secondly, the combination partners of hydrocolloids and flavonoids against the formation of heterocyclic amines and advanced glycation end products were also find out; Lastly, the potential mechanism involved was clarified by evaluating the influence of both physical properties and chemical interactions.