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Supporting data for "MAMERR Interacts with HSF2BP and BRCA2 to Regulate the Ubiquitination of Mouse Pachytene Spermatocytes"

dataset
posted on 2024-10-18, 06:39 authored by Binjie JiangBinjie Jiang

This study is associated with the meiosis process, especially the pachytene stage of meiosis prophase I. In this study, we found a new function of MAMERR in mouse pachytene spermatocytes. Despite of function as a recombination regulator, MAMERR also plays a role in the transcription regulation and ubiquitination process of mouse pachytene spermatocytes. Deletion of MAMERR causes transcription dysregulation and enhanced ubiquitination, and MSCI suffers defects, eventually resulting in spermatogenesis defects.

The dataset associated with this study is put in the main folder. The main folder contains 6 sub-folders and the sub-read-me file is prepared for each sub-folder. 6 sub-folders match the 6 parts in the thesis. The results mainly contain immunoblotting, IHC, IF, HE staining, IP assays, etc. All results can be found in the figure files. For RNA sequencing and ubiquitination-mediated mass spectrometry, the Excel files are also prepared in the matching sub-folders. The figure files contain the legend details for easy reading.

Dataset 3.1 mainly focuses on the expression pattern of MAMERR. Generation and verification of the MAMERR KO mice model. The exact stage of cell apoptosis.

Dataset 3.2 mainly illustrates the flowchart of pachytene spermatocyte isolation and RNA sequencing of isolated pachytene cells.

Dataset 3.3 contains the immunoblotting analysis of isolated pachytene cells' ubiquitination. Generation and verification of MAMERR Overexpression mice model.

Dataset 3.4 almost illustrates the ubiquitination-mediated mass spectrometry data from testes and isolated pachytene cells.

Dataset 3.5 mainly focuses on HEK293T transfection experiments, IP assays, and DUBs assays.

Dataset 3.6 includes a scheme of the potential mechanism of MAMERR's function in pachytene spermatocytes.

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