<p dir="ltr">Previous methods for measuring energy cofactors in plant cells were mostly indirectly and required the extraction of these cofactors before measurements, making it inaccurate and impossible to observe dynamic changes in the subcellular levels of these molecules. In this study, by analyzing the tissue-specific expression patterns of genes encoding cytosolic NADPH-producing enzymes using the GUS reporter system, introducing a novel fluorescent energy sensor mCherry-iNAP1 into several Arabidopsis mutants, and comparing cytosolic NADPH levels in differenttissues of wild type (WT) and mutants, the sources of cytosolic NADPH in different Arabidopsis tissues are clarified.</p>
Funding
Hong Kong Research Grants Council General Research Fund (17102322)
Area of Excellence Scheme (AoE/M-403/16)
Innovation and Technology Fund (Funding Support to State Key Laboratory of Agrobiotechnology) of the Hong Kong Special Administrative Region, China
National Natural Science Foundation of China (32070394)