Supporting data for "Streptococcus pyogenes Cas9 (SpCas9) and Staphylococcus aureus Cas9 (SaCas9) Engineering with Enhanced Genome Targeting Accuracy"

Cas9 engineering were conducted on SpCas9 and KKH-SaCas9 that contributed to two specificity improved variants: Opti-SpCas9 and KKH-SaCas9-SAV1. Experiments including GFP disruption assays and T7E1 assays were conducted for editing activity characterizations. Experimental data of the engineered Cas9 variants are included here.