The investigation into how NS1 regulates its mRNA splicing and nuclear exportation has unfolded across decades and yield diverse insights. The findings emphasized that NS1 controls its own mRNA splicing and nuclear export via m6A modification. The conservation of the regulatory m6A site (A385) highlights its significance as a key target for future antiviral interventions. This file contains IFA experiment results, western blot images, and RT-qPCR data that support this project.