Supporting data for "The Mevalonate Pathway: A Key Player in Protecting Hepatocellular Carcinoma from Ferroptosis"

My studies explore the underlying mechanism of how the Mevalonate pathway supported liver cancer against ferroptosis via CoQ10 production and selenoprotein translation. We identified MVD as an NRF2 target. Moreover, MVD knockdown suppressed the metabolic flux of the Mevalonate pathway which was indicated by LC-MS detection. In the dataset, qRT-PCR analysis was performed to study mRNA expression of MVD to investigate its regulation by NRF2/KEAP1 pathway. ChIP assay was used to study the binding of NRF2 to MVD. LC-MS was applied to detect the metabolites of the Mevalonate pathway.