posted on 2024-01-04, 03:33authored byMiaojia Hang
<p dir="ltr">The function and molecular mechanisms of a novel kinase protein Src Homology 3 Domain Binding Kinase 1 (SBK1)in the liver under fasting conditions. The supporting data was collected from different experiments. Gene expression profiling in tissues was performed using RNA sequencing, microarray, and real-time PCR. Activation of intracellular signaling pathways was determined by Western blotting. Dual-luciferase assay was used to characterize the promoter and responsive element activities. FAO in cells and tissues was measured using <sup>14</sup>C-palmitate oxidation. Protein-protein interactions were investigated by GST pull-down and immunoprecipitation.</p>