File(s) under embargo
Supporting data for "The identification and characterization of histone chaperone ASF1B in the regulation of tumor lineage plasticity in hepatocellular carcinoma".
Hepatocellular carcinoma (HCC) ranks as the fifth most common cancer and the second leading cause of cancer-related mortality worldwide. The overall prognosis of HCC patients is very poor because it is susceptible to recurrence and metastasis. According to the cancer stem cell (CSC) model, there is a small subset of cancer cells within the tumor bulk that is responsible for tumor relapse and metastasis. CSCs represent cells that can self-renew, initiate tumors, and keep resistant to anti-cancer treatment. However, the fate and signatures of CSCs in HCC remain elusive. This study mainly focused on the key gene and its regulatory mechanisms that are responsible for the maintenance of HCC stemness. We found that histone chaperone ASF1B was one of the key genes and demonstrated its functions in HCC. This attached dataset included the raw data of "The identification and characterization of histone chaperone ASF1B in the regulation of tumor lineage plasticity in hepatocellular carcinoma", including RNA-sequencing data, data of qPCR, IF, HE, and IHC staining, results of in vitro functional assays including foci formation, spheroid formation, migration, invasion, and cell viability assays, as well as in vivo mouse experiments using different mouse models and CHIP-qPCR result and so on.