Supporting data for "The role of ACSS2 in early transcriptional regulation during reversible induction"

This dataset contains raw and processed data for ChIP-seq, chrRNA-seq, IFA, qPCR, Western blot, and other analysis used in the thesis entitled "The role of ACSS2 in early transcriptional  regulation during reversible induction".

Histone acetylation is an active histone mark for active enhancers or promoters, promoting gene expression. The process is catalysed by lysine acetyltransferases, which are able to bind to specific DNA sequences via transcription factors, and transfer the acetyl-group from acetyl-CoA to lysine residues of histone tails. Recently, there is evidence suggesting that acetyl-CoA providing enzymes may have specific chromatin-binding ability to regulate gene expression as well. 

In this project, we will dissect the role of acyl-CoA synthetase short-chain family member 2 (ACSS2) in transcriptional reprogramming of reversible inductions, with glucocorticoid signaling as an example using high-throughput technologies. The specific chromatin-binding patterns and transcription modulating effect of ACSS2 will first be investigated by ChIP-seq and chrRNA-seq techniques respectively. The interplay and recruitment dynamics between ACSS2, other histone modifying enzymes and transcription factors will be compared to understand the cascade of transcription activation. In summary, this thesis comprehensively evaluates the temporospatial changes and functional impacts of ACSS2 in early glucocorticoid receptor signalling..