2 files

Supporting data for “Threats and Conservation of the Critically Endangered Helmeted Hornbill (Rhinoplax vigil)”

posted on 09.11.2021, 02:30 authored by Chloe Emma Ruth WebsterChloe Emma Ruth Webster, Caroline Elise Dingle

This genetic and morphological data was generated from Critically Endangered helmeted hornbill casques, which were seized by the Agricultural Fisheries and Conservation Department (AFCD) of Hong Kong SAR. The casques from this bird are illegally poached and illegally traded, and developing genetic and morphological protocols to identify them can aid conservation and enforcement efforts. Chapter 1: DNA samples taken from six R. vigil casques to test which extraction method (Q1), sampling method (Q2), and sampling location (Q3) resulted in the best quantity (DNA concentration ng/μL and quality DNA). DNA fasta sequences were generated for R. vigil and positive control Rhyticeros undulatus hornbills. Samples extracted using phenol-chloroform methods and using Qiagen DNeasy Blood and Tissue kit and an 800-bp of cytochrome B was amplified. Chapter 2: DNA concentrations and purities between replicate Rhyticeros undulatus hornbill faecal samples were extracted using phenol-chloroform and Qiagen stool kit methods and compared between storage media. A260/A280 and A260/A230 ratios which fall under target ranges optimal ranges of purity values are in bold. DNA concentrations (ng/µL) and purities (nm) (means and +/- standard deviations) and sequencing success (%) were also generated across faecal samples from R. plicatus, B. bucinator, B. brevis, R. undulatus, and B. bicornis extracted using the QIA method across storage times: day, week, month, and storage media. A260/A280 and A260/A230 ratios (showing DNA purity) which fall under target ranges optimal ranges of purity values are in bold. Chapter 3. Morphometrics were recorded for hornbill casques to the nearest 0.1 mm using digital callipers: weights (g), the top of casque to join at top of beak (A), the top of casque to bottom of beak (B), the width of beak-casque join (C), and the casque width between ridges (taken underneath ridge, D). Adults and juveniles were identified, and so were their sexes.


Ocean Park Conservation Foundation, Hong Kong. Grant # BD02.1819