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Supporting data for the application of CRISPR-mediated genome editing to study the pathophysiological role of proteins related to neurological disorders
datasetposted on 2021-08-10, 08:24 authored by Quanwei Lyu
The dataset includes the data for the thesis of my Ph.D. study. It mainly includes three parts. The first part is related to the characterization of the hiPSC-derive cortical neurons, which consists of confocal images of immunostaining, parch clamp records, and quantitative analysis results. The second part is related to the verification of the genome-edited hiPSC clone and characterization of the edited cells and edited hiPSC-derived cortical neurons. This part includes DNA sequencing result, gel-electrophoresis result, and confocal images of immunostaining. The last part is related to the edited hiPSCs carrying various disease-related mutations respectively. These cells were differentiated into cortical neurons, and this part of data is to compare/ describe the phenotypes between the cells carrying various disease-related mutations. This part of data includes DNA sequencing result, gel-electrophoresis result, western blot analysis, and confocal images of immunostaining.