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Supporting data of Long-read sequencing uncovers alternative transcript isoforms essential for hypoxic adaptation in hepatocellular carcinoma

dataset
posted on 2023-08-25, 03:45 authored by Ting Chi Rebecca Wan

This dataset contains the excel file with numerical data used to plot the indicated figures in a thesis about utilizing long-read sequencing to identify isoforms in liver cancer cell lines under hypoxic stress conditions. Dataset includes (1) cell proliferation data by cell counting on cells with perturbed gene expression or incubated in different stress conditions; (2) Quantification on differentially expressed genes and isoforms identified in long-read sequencing; (3) qPCR results in isoforms and splicing events validation; (4) Quantification on hypoxia-induced splicing events on previously published HCC cohort; (5) qPCR results of luciferase assay and polysome fractionation experiments; and (6) Quantification results of immunofluorescent staining in colocalization of with different organelles.

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