.GEL

ePOU_150nM_Can_S2_S17_20200310-[Cy5]-A.gel (45.78 MB)

.GEL

ePOU_150nM_Comp_S17_S2_20200310-[Cy5]-B.gel (45.78 MB)

.GEL

ePOU_300nM_MOREA4+1_20200312-[Cy3]-B.gel (45.78 MB)

IMAGE

ePOU_300nM_MOREC4_20200305-[Cy5].tif (2.48 MB)

DATASET

Heterodimer_EMSAs_Cooperativity.csv (7.99 kB)

DATASET

Homodimer_EMSAs_Cooperativity.csv (9.43 kB)

.GEL

Oct4_150nM_Can_50nM_S2_S17_25nM_20200310-[Cy5]-1-A.gel (45.78 MB)

.GEL

Oct4_150nM_Comp_S17_S2_20200310-[Cy5]-1-B.gel (45.78 MB)

.GEL

Oct4_300nM_MOREA4+1_20200312-[Cy3]-A.gel.gel (45.78 MB)

DATASET

KDEMSA_Oct4__20200225.csv (0.31 kB)

DATASET

KDEMSA_SOX2_24102018.csv (0.32 kB)

.GEL

KDEMSA_SOX2_24102018.gel (1.03 MB)

DATASET

KDEMSA_ePOU_20200225.csv (0.34 kB)

.GEL

KDEMSA_ePOU_Oct4__20200225.gel (91.56 MB)

TEXT

Annotate_Shared_ATAC_D3_peaks.txt (54.83 MB)

TEXT

Annotate_Shared_ChIP_D3_peaks.txt (1.15 MB)

TEXT

Anntotate_ePOU_ChIP_D3_peaks.txt (982.41 kB)

TEXT

Annotate_ESKM_ATAC_D3_peaks.txt (2.76 MB)

TEXT

Annotate_Oct4_ChIP_D3_peaks.txt (228.8 kB)

TEXT

Annotate_OSKM_ATAC_D3_peaks.txt (1.3 MB)

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53 files

The biochemical and biophysical basis for the enhanced reprogramming activity of an artificially enhanced Pit-Oct-Unc transcription factor - ePOU

dataset

posted on 2020-09-08, 02:14 authored by Daisylyn Senna Tan

This is the data set used for the MPhil thesis of the same title. Background: Cellular reprogramming has the potential to revolutionize medicine through personalized disease models, ex vivo drug testing and regenerative therapies. Our group engineered the scaffold of Pit-Oct-Unc (POU) factor in the context of iPSC reprogramming and identified an enhanced POU factor (ePOU) that substantially outperforms wild-type Oct4 in terms of speed and efficiency. However, the mechanism behind its enhanced ability is unknown. Here, I try to understand reprogramming through comparing and contrasting ePOU with Oct4 through biochemical and biophysical means.