The biochemical and biophysical basis for the enhanced reprogramming activity of an artificially enhanced Pit-Oct-Unc transcription factor - ePOU
datasetposted on 08.09.2020 by Daisylyn Senna Tan
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
This is the data set used for the MPhil thesis of the same title. Background: Cellular reprogramming has the potential to revolutionize medicine through personalized disease models, ex vivo drug testing and regenerative therapies. Our group engineered the scaffold of Pit-Oct-Unc (POU) factor in the context of iPSC reprogramming and identified an enhanced POU factor (ePOU) that substantially outperforms wild-type Oct4 in terms of speed and efficiency. However, the mechanism behind its enhanced ability is unknown. Here, I try to understand reprogramming through comparing and contrasting ePOU with Oct4 through biochemical and biophysical means.