Radiographic assessment is the gold standard for the diagnosis and prognosis of adolescent idiopathic scoliosis (AIS), while radiation exposure remains a major concern. Surface topography has been widely researched to reduce the reliance on radiographs for managing AIS. However, a practical solution has yet to be defined for regular clinic use. SpineScan3D is a novel handheld smartphone-based surface profiling tool with a low manufacturing cost, which has a gyroscope and accelerator embedded for assessing surface topography. The current work aims to investigate the clinical reliability and validity of the device in AIS subjects, followed by its value of monitoring curve progression in a prospectively longitudinal manner.