<b>Supporting Data for Functional Studies on Genomic DNA Organization in Female Reproductive Cells — Effects of Condensin-mediated Chromatin Assembly and G-quadruplex Structures</b>
<p dir="ltr">Female reproduction is a continuous and precise life process involving oocyte maturation supported by granulosa cells, fertilization, and early embryonic development, which ensurs the stable inheritance of genomic information. This study comprehensively investigated regulatory functions of genomic DNA organization in female reproductive cells, focusing on two extreme levels covering the entire DNA organizational levels from DNA bases to chromosomes: (i) Microscopic level: DNA secondary structure (G-quadruplexes), and (ii) Macroscopic level: the large-scale chromatin compaction driven by architectural proteins (condensins). By studying the two vastly different levels of genomic organization, we aim to unravel how distinct structural principles collectively maintain genome integrity and functionality. Disruption of either mechanism compromises chromatin integrity, causing DNA damage, impaired replication/transcription, and defective cell cycle progression, ultimately leading to developmental failure and female infertility.</p><p dir="ltr"><b>The dataset contains the figures data for this thesis, structured into folders corresponding to each chapter:</b></p><p dir="ltr">Chapter 2: Essential Role of SMC2-Mediated Chromosome Compaction in Mouse Oocyte Meiosis and Early Embryonic Development;</p><p dir="ltr">Chapter 3: Regulatory Effects of G-Quadruplex Structures on Mouse Oocyte Development via R-loop;</p><p dir="ltr">Chapter 4: G-Quadruplex Stabilization Impairs Early Embryo Development by Impairing DNA Replication and Inducing R-Loops Accumulation;</p><p dir="ltr">Chapter 5: G-quadruplex Stabilization Triggers R-loop Accumulation, DNA Damage and Telomere Shortening in Human Granulosa Cells.</p>
