Supporting data for "Effects of Akkermansia muciniphila on Porphyromonas gingivalis-Induced Periodontal Disease: Immune Modulation and Microbiota Remodeling"

The Dataset include the Elisa, qPCR, and WesternBlot result for the following study.

The experimental section investigates the effects of Am on host immune responses and microbial interactions using both in vitro and in vivo models. In vitro studies with human gingival epithelial cells and human gingival fibroblasts demonstrate that Am can restore immune responses suppressed by Pg, enhance the expression of pro-inflammatory cytokines (IL-6, IL-8, MCP-1), and promote the recruitment of immune cells. Additionally, Am significantly reduces the adhesion and invasion of Pg, enhancing the resistance of host cells to infection. In vivo studies using a murine model of periodontitis reveal that Am administration mitigates alveolar bone resorption and reduces bacterial load in the periodontal compartment. The study shows that Am enhances the phagocytic activity of THP-1 differentiated macrophages via the MyD88 pathway, disrupting the TLR2/C5aR interaction exploited by Pg and promoting bacterial clearance.