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Supporting data for “Roles of MAPS and SPDYA in XY Chromosomal Dynamics during Male Pachynema Progression

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posted on 2024-10-18, 06:39 authored by Dongliang LiDongliang Li

This project selected two genes related to pre-meiotic prophase (Maps and Spdya) for study. We constructed models using transgenic mice, including models with complete knockouts, conditionally induced knockouts, and heterologous expression post-knockout, to further investigate their roles during the leptotene stage of male meiosis. For the maps project, we have also confirmed in clinical settings that mutations in this gene lead to azoospermia, consistent with findings in the mouse model. All other experiments were conducted in mice and cell lines. Routine molecular biology experiments were independently repeated three or more times. High-throughput sequencing techniques, such as RNA-seq and proteomics, were performed at least once. For experiments that only involved a single instance of mass spectrometry, other methods were used for validation.

The first chapter is the introduction, which includes authorized images and copyright information. The second chapter is the method section, which details the most basic and common experimental procedures used in this study. The third and fourth chapters present the editable results for the maps and spdya projects, respectively.

Funding

the Shenzhen Science and Technology Program (KQTD20190929172749226 to K.L. and W.S.B.Y.)

the National Natural Science Foundation of China and the Swedish Research Council Collaboration Research Program (NSFC-VR 8211101255 to K.L.)

the National Natural Science Foundation of China (82071706 to K.L.)

the Hong Kong Research Grant Council (17114920 to K.L.)

the Shenzhen-Hong Kong Innovation Circle Type C (201908163000525 to K.L.)

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