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Supporting data for " Investigation on the microbiota, lactate and pH on endometrial receptivity and pregnancyoutcome prediction"

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posted on 2025-01-09, 09:14 authored by Manish Kumar .Manish Kumar .

Globally, infertility affects 48 million couples and 186 million individuals, with one in six couples in Hong Kong and one in eight in the UK impacted (WHO, 2020). Major causes include maternal age, poor oocyte quality, tubal blockage, and male factor infertility. Recent research highlights the role of a lactobacillus-dominant (LD) uterine microbiome in influencing embryo implantation and IVF outcomes. Lactobacilli produce lactic acid and hydrogen peroxide, which suppress pathogenic bacteria, lower vaginal pH, and maintain a healthy microbiota. This study investigated the effects of lactate (D- & L-form) and pH on embryo implantation using a spheroid-endometrial co-culture model. High lactate concentrations (>20mM) reduced cell viability, proliferation, and trophoblastic BeWo cell invasion, as well as angiogenesis in HUVEC cells. While pH conditions (6.1-8.1) did not affect spheroid attachment, high lactate levels induced cytokines (e.g., TIMPs, MMPs, IL6, IL6R) that may influence endometrial receptivity and immune responses. These findings suggest that the LD microenvironment may promote pregnancy by modulating cytokines and secretome to enhance endometrial receptivity.

Funding

Hong Kong Government HMRF (06173976)

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