<em>Supporting data for</em> “Peroxidase- and Photosensitizer-Mediated Proximity Labeling for Mitochondria-associated Transcriptome Analysis in Live Cells”
posted on 2023-07-21, 03:00authored byJiying Liang
<p>Folder 1: Confocal microscope data</p>
<p>Includes confocal images in tif format.</p>
<p>Short description: The data were collected using confocal microscope (Leica) to examine the colocalization of labeling sites and commercial organelle trackers. </p>
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<p>Folder 2: Nuclear magnetic resonance spectroscopy (NMR) data</p>
<p>Includes proton (1H) and carbon-13 (13C) spectra in MNOVA format.</p>
<p>Short description: NMR data were collected using Bruker Advanced spectrometers to characterize the purity of our probes, and all NMR spectra were analyzed using MestReNova reader software.</p>
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<p>Folder 3: UV-Vis absorption spectra data</p>
<p>Includes absorbance analysis of our probes in xlsx format.</p>
<p>Short description: UV-Vis absorption spectra were collected using Varian Cary 50 spectrometer to provide information of excitation wavelengths.</p>
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<p>Folder 4: Spectrofluorometer (FL) data</p>
<p>Includes: FL spectra data in xlsx format.</p>
<p>Short description: FL spectra data were collected using the FS5 spectrofluorometer (Edinburgh Instruments).</p>
<p>Probes displayed the fluorescence intensities and peaks.</p>
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<p>Folder 5: Dynamic light scattering (DLS) data</p>
<p>Includes: DLS data in xlsx format.</p>
<p>Short description: DLS (Zetasizer Pro, Malvern, Germany) was utilized to determine the size distribution of our probes.</p>
<p>The ZS Xplorer software was used to analyse the hydrodynamic diameter of each test.</p>