The dataset established as part of the research on HAUS7 in esophageal squamous cell carcinoma (ESCC) elucidates the involvement of HAUS7 in ESCC progression through its interaction with γ-tubulin facilitated by the novel peptide HTIP1. The dataset incorporates raw and processed data encompassing diverse experimental modalities including western blotting, immunohistochemistry, microscopic imaging, and qPCR assay. Utilizing Prism software for analysis, the dataset encompasses comprehensive information on gene and protein expression profiles, cellular viability assessments, apoptotic assays, as well as observations on tumor growth and survival parameters derived from patient-derived xenograft models.