Supporting data for “Functional characterization of low-density lipoprotein receptor-related protein 1B (LRP1B) and Mitotic arrest deficient 2 like 2 (MAD2L2) in esophageal squamous cell carcinoma (ESCC)”

This study aimed to elucidate the underlying molecular pathogenesis of ESCC by investigating the functions of the two critical genes, Mitotic arrest deficient 2 like 2 (MAD2L2) and Low-density lipoprotein Receptor Related Protein 1B (LRP1B), with frequent alterations in its development. lenti-CRISPR-Cas9 was used to depletion the expression of the genes. The depletion effects were examined by in vitro and in vivo subcutaneous tumorigenesis assay. RNA-seq was used to explore the downstream factors of the genes. RT-qPCR was used to validate the expression of the immune cell markers and the immune response genes. These experiments aim to examine the effect of the depletion of these two genes on ESCC cell lines and ESCC tumor microenvironment.