HKU Data Repository
Browse

File(s) under embargo

Reason: Unpublished

2

month(s)

6

day(s)

until file(s) become available

Supporting data for "Development of RNA Proximity Labeling Approaches Mediated by Fluorogenic Photosensitizers"

dataset
posted on 2023-06-23, 06:53 authored by Lan Li

This dataset contains four subfiles, including 1) Organic synthesis of fluorogenic photosensitizers. Detailed synthetic routes of a series of malachite green derivatives and Hoechst-conjugated dibromofluorescien are recorded with the purification processes. 2) In vitro photophysical characterization of the synthetic compounds and the complex forming by binding to protein and DNA, including the UV-Vis absorption spectra, fluorescence spectra, and ROS detecting assays; 3) bacteria-related experiments which detailly record plasmid construction, amplification, protein expression, and purification; 4) mammalian cell-related experiments, including the microscope imaging of the synthetic compounds in live cells and visualization of the in situ labeling, analysis of protein and RNA isolated from labeled cells via western blot, in-gel fluorescence, dot blot, etc..

Funding

Near-infrared light inducible toolbox for RNA localization studies in living cells

University Grants Committee

Find out more...

An integrative approach to study RNA/protein translocation in living cells

University Grants Committee

Find out more...

History

Usage metrics

    Research Postgraduates

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC