Supporting data for "A role of biglycan C-terminal peptide in regulating intervertebral disc homeostasis"

Intervertebral discs (IVDs) are essential to spinal motion. IVD degeneration (IDD) is associated with aging and chronic inflammation, involving excessive degradation and remodeling of the extracellular matrix (ECM). However, the mechanism of IDD pathogenesis is not yet fully understood, and effective treatments for IDD remain to be investigated. Biglycan (BGN) is a member in the first class of small leucine-rich proteoglycans (SLRPs) that have been suggested to have diverse biological functions predominantly in the ECM. Nevertheless, the role played by changes in BGN activity during IDD so far remains elusive. This study is the first to identify the important role of Bgm1 in IVD development and IDD progression.

All data included in this dataset such as in vitro results, in vivo validation, transcriptomics and proteomics results. All experimental data including Western Blotting, RT-qPCR, immunostaining, histological staining, RNA-seq data analysis, Proteomic data analysis, and Illustration figure are presented in jpg format. All experiments involving patients or animals were ethically approved.