This study not only reveals the potent antibacterial properties of OPV but also underscores its potential as a safe and effective treatment for caries and root canal infections. By effectively targeting and eradicating bacteria within biofilms, OPV offers a promising alternative to conventional treatment modalities, thereby addressing the growing concern regarding antibiotic resistance. This study bridges the gap between materials science and microbiology and paves the way for innovative therapeutic strategies against challenging bacterial infections.