Supporting data for "Cross-lineage induction of murine totipotency via epigenetically reprogramming of GC-rich retrotransposons "

Totipotent cells can give rise to all the differentiated cells in both embryonic and extraembryonic tissues.Despite the central importance of totipotency in establishing life, how does a totipotent embryo arise from two terminally differentiated gametes and the key factors and molecular mechanism by which the embryonic chromatin is naturally reprogrammed to a totipotent state to trigger zygotic genome activation (ZGA) are largely unknown in mammals.Here, from pluripotent stem cells, we have identified a single molecule that enables establishment of totipotent zygotic genome activation-like cells (Z-cells) with high potency of generating both embryonic and extraembryonic cell lineages in in vivo chimeras and in vitro differentiation and blastoid generation.