Supporting data for “Utilizing Synthetic Biology Strategies for the Development of Attenuated Vaccines Targeting Staphylococcus aureus and Pseudomonas aeruginosa”

The current study aims to design attenuated live bacterial vaccines against Staphylococcus aureus and Pseudomonas aeruginosa. Additionally, efforts were made to investigate the immune responses induced by the vaccine candidates in order to identify the immunity that confers protective effects.

In this study we utilized a synthetic biology approach to design attenuated Staphylococcus aureus and Pseudomonas aeruginosa vaccine candidates. The growth capacity and morphology of the engineered bacterial vaccine candidates were characterized in bacterial culture and transmission electron microscopy. Their safety and protective efficacy were then validated in animal models. Immune responses during vaccination and against wild-type strain infections were investigated by flow cytometry. The dataset here resembles the above-mentioned measurement, including figures of growth cure, electron microscope images, flow cytometry plots, statistical analysis on flow cytometry data and antibody titration, survive curve.