Supporting data for "The Inhibitory Effects of Biomimetic Strategies on Streptococcus Mutans Biofilm Formation"

The biomimetic strategies explored in this thesis include graphene oxide (GO), graphene quantum dots (GQDs), Ti-Cu alloy, and titanium nitride (TiN) nanoparticles under near-infrared (NIR) light irradiation. Following synthesis, the antibacterial efficiency of these materials was assessed through various tests, including anti-planktonic S. mutans, anti-biofilm S. mutans, and anti-S. mutans adhesion assays. The anti-planktonic and anti-adhesion efficiencies were evaluated using colony-forming unit (CFU) counts, scanning electron microscopy (SEM), and Live/Dead staining. Crystal violet staining was employed to assess anti-biofilm capabilities. The antibacterial mechanisms were investigated by measuring reactive oxygen species (ROS) levels and adenosine triphosphate (ATP) concentrations to elucidate bacterial cell responses. Additionally, biocompatibility was evaluated by examining the proliferation of different cell lines.