Supporting data for “Elucidating the extra-embryonic differentiation potential of human Expanded Potential Stem Cells”

ISL1 was selected for reverse genetic study. An ISL1-EGFP reporter hEPSC line was derived and their differentiation in hTSCM revealed the inter-cellular heterogeneity in ISL1 protein levels, which were undetectable in primary hTSC-like colonies. These dynamics appeared as altered by BMP4 or LDN193189 supplement. Also, stable ISL1^+/+, ISL1^+/-, and ISL1^-/- hEPSC lines were derived but ISL1-KO did not impact their pluripotency or extra-embryonic differentiation. The function role of ISL1 in hEPSCs awaits further investigation.