<i>Supporting data for “Elucidating the extra-embryonic differentiation potential of human Expanded Potential Stem Cells”</i>

<p dir="ltr"><i>ISL1</i> was selected for reverse genetic study. An <i>ISL1-EGFP</i> reporter hEPSC line was derived and their differentiation in hTSCM revealed the inter-cellular heterogeneity in ISL1 protein levels, which were undetectable in primary hTSC-like colonies. These dynamics appeared as altered by BMP4 or LDN193189 supplement. Also, stable <i>ISL1</i>^+/+, <i>ISL1</i>^+/-, and <i>ISL1</i>^-/- hEPSC lines were derived but <i>ISL1</i>-KO did not impact their pluripotency or extra-embryonic differentiation. The function role of <i>ISL1</i> in hEPSCs awaits further investigation.</p>