FastQCFastQC Report
Tue 9 Jul 2019
SL_2_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SL_2_R2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 40326607
Filtered Sequences 0
Sequence length 100-150
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGGTG 17683485 3.534871 23.872059 6
TTGGT 20613855 3.3156908 23.2491 5
TGCTG 13561660 3.2335408 17.890358 9
TGAAG 24569745 3.1250913 10.369801 9
TCTTG 16144030 3.0973227 23.52629 9
CTTGA 17137715 2.923823 5.162125 7
TGGCT 11694940 2.7884538 12.5166235 9
CCTTC 8094325 2.7457788 6.2258472 65-69
CTTGG 11449780 2.73 13.075688 3
TGATG 18384025 2.6295354 28.292803 2
GCTCT 9217655 2.6214745 12.035605 2
TCAAG 17124865 2.598059 5.604899 9
TTGCT 13514455 2.5928242 19.918312 4
TTGAA 25195560 2.5786664 13.456092 8
GCCGA 8038805 2.5265698 244.6032 1
GTCGT 10395445 2.4786122 7.858285 4
CTGGT 10382430 2.475509 17.939018 4
TCGTG 10270365 2.4487891 9.2612915 6
CGTCG 6904165 2.4402108 8.144453 145-146
TGCTA 13804250 2.355109 12.901115 9
AAGGT 18363510 2.3357038 7.6588182 1
TTGAC 13673195 2.3327498 6.5335884 8
GGTGA 12972030 2.3058832 7.403421 7
ATGCT 13439690 2.2929122 7.854752 15-19
TTCAA 18572615 2.2672734 9.139889 8
CTCTT 9703095 2.2204678 10.248558 8
ACTTG 12735810 2.1728249 11.293583 4
TGGTC 9100915 2.169954 11.815035 6
GTTGC 9058020 2.1597266 24.757341 3
TGGTA 14922830 2.1344678 8.204219 6
CTGAA 14048585 2.131348 8.059964 8
TGAAA 23411130 2.1306744 15.796509 9
CTGCT 7461395 2.1219993 27.675016 4
TCTTC 9131660 2.0897 11.951683 9
ACAAC 12844465 2.066908 9.510702 4
CTTCT 8965850 2.0517557 15.299472 5
GGTGG 8025820 1.9938209 7.964535 7
TGCCA 7846340 1.9843395 12.371068 9
GTGGT 9911525 1.9812815 6.550896 8
CAACT 10825710 1.9590156 14.23054 5
GTCAA 12781525 1.9391189 7.179869 8
CCTTG 6794085 1.9322182 7.6647277 6
GTCTT 10005680 1.9196458 13.270636 8
TTGCC 6711440 1.9087144 12.340005 8
TGGTT 11815085 1.9004289 10.602238 5
CAGCC 5066165 1.8992355 6.3092995 60-64
TTTGG 11792860 1.8968542 11.230118 7
TGCAG 8860060 1.8785632 8.919482 9
TTGGC 7784635 1.8561101 12.5495825 8
CTGGC 5214325 1.8429532 7.2999578 8
CCAAG 8187430 1.841281 9.623283 1
ATGGT 12871065 1.8409964 14.091954 5
TCGTC 6442370 1.8321915 7.7886286 1
GCCTA 7243680 1.8319267 146.27417 2
ACTGG 8629705 1.8297219 15.953951 3
GAAGG 11569330 1.8287791 8.98828 3
TCATC 8830340 1.796947 5.5531774 4
CTCAA 9841815 1.7809705 5.680037 8
TTTGA 15422745 1.7750441 9.5665455 7
CGTTG 7408285 1.766376 16.188152 1
TGCCG 4932175 1.74323 11.259431 9
GGTGC 5860210 1.7364793 10.268265 7
TCAAA 15968690 1.7334996 7.5119624 9
CTTGC 6090530 1.7321292 8.201079 7
GTTGG 8654645 1.7300355 13.578747 2
AGCTC 6829650 1.7272186 10.705403 1
GGCTC 4864055 1.7191534 6.059272 10-14
GCTGT 7191500 1.7146875 7.2146726 4
TCAGC 6774745 1.713333 13.148537 9
CTCGT 6015355 1.7107496 7.6071 4
TGTTG 10582965 1.7022452 23.708307 9
AACGT 11160050 1.693121 12.500298 5
GTGAA 13300895 1.6917764 11.445009 8
TGACT 9828760 1.6768602 13.807233 5
TTATC 12184935 1.6727475 12.328837 6
AATTG 16339500 1.6722834 15.868063 6
ACCTT 8167030 1.6619655 10.539816 5
TGCTC 5837075 1.6600473 8.161358 9
GGTAA 13018695 1.6558826 5.0463233 7
CCGAT 6545285 1.6553026 197.12103 2
TTCTT 10661750 1.6459326 18.039658 8
AACTG 10746490 1.6303786 11.406547 2
CGTCA 6404595 1.6197221 16.788263 2
TCTCA 7939700 1.6157044 14.850641 9
ATTGC 9450375 1.612305 13.172 7
CTGCA 6366910 1.6101916 5.8010087 8
GACTG 7572810 1.6056327 7.991169 6
TTCCA 7863080 1.6001127 15.712749 3
GTGAC 7525365 1.595573 17.525331 4
CGAAG 8449585 1.5931189 10.71516 2
TTGGA 11090295 1.5862862 10.06194 8
CGTGA 7402145 1.5694474 7.555706 7
GGTGT 7829810 1.5651535 16.22766 7
GATGA 12272530 1.5609759 9.055547 3
ACTTC 7620700 1.550789 13.886036 4
CTTCG 5442305 1.5477759 6.177309 1
GGTTT 9601290 1.5443451 6.578644 5
GGTAC 7275680 1.5426333 12.882537 3
TCAAC 8435160 1.5264227 7.8928328 9
TTTCA 11089395 1.5223519 8.891212 7
GTGCC 4275280 1.5110565 9.977716 8
TTCTC 6588000 1.5076058 12.818316 8
AGCTA 9927200 1.5060819 10.629766 1
CATCG 5954280 1.5058373 7.8427815 3
GGTTG 7526820 1.5045868 20.79104 2
ATTGA 14658520 1.5002416 10.194649 7
GTGCT 6229595 1.485338 14.031443 8
TTGTC 7694770 1.4762849 19.400288 8
GCTTC 5180625 1.4733548 12.424799 6
TATCG 8575255 1.4630028 6.69323 9
TGCTT 7621205 1.462171 37.34786 5
ATCGG 6889695 1.4607946 6.8504887 145-146
TATTG 12681355 1.4595304 21.127993 6
TTCGT 7548180 1.4481606 14.201858 5
GTTCA 8450905 1.4417878 9.657904 1
GGTAT 9980210 1.4275064 39.01297 3
CATGG 6725880 1.4260615 21.401693 4
ATTGG 9904455 1.4166712 16.942455 4
GCGGT 4767195 1.4126006 80.633896 1
CGTGG 4741665 1.4050357 8.393414 7
GTGGC 4741665 1.4050355 10.273625 8
TTCAG 8188950 1.3970965 8.840309 8
GGTCA 6589195 1.3970807 6.35749 7
TCGTA 8173920 1.3945322 5.428937 1
CGGTA 6511565 1.3806211 57.80402 2
TCGTT 7188050 1.3790677 11.344465 2
GTGAT 9628620 1.3772173 22.018866 1
CGTTC 4825485 1.372354 21.972525 1
CTGTT 7151815 1.3721159 14.279193 8
AACCT 7571505 1.3701361 32.8741 5
CAAGG 7266920 1.3701344 8.075189 2
GCTTG 5742880 1.3692894 22.277103 6
ATCGT 7986610 1.3625757 5.3026295 4
GTTCT 7053035 1.3531642 18.612076 1
CGTGC 3818280 1.3495345 11.162234 7
GCTAC 5319390 1.3452737 17.653399 2
GTATT 11674130 1.343606 64.617424 4
TAAAA 20557070 1.3387166 7.449547 9
CTCAG 5288795 1.3375363 5.4679117 8
GTAAA 14692425 1.3371748 6.08164 8
ATCTT 9717705 1.3340461 6.6486382 8
GACTT 7795195 1.3299189 22.941517 3
GAACC 5896220 1.3260082 41.393513 4
TGAAC 8707335 1.3210127 5.9248333 9
TTGCA 7706205 1.3147365 9.97447 8
AGGTT 9137740 1.3070049 14.962072 1
TGGAA 10255150 1.3043799 14.004024 9
CGGTG 4400535 1.303953 15.702615 6
CTGAT 7594115 1.295613 7.4581046 1
CACAG 5757700 1.2948562 5.9928045 2
AGGTA 10170125 1.2935654 7.7403727 2
GGCCT 3629600 1.2828472 121.06011 1
ATTTG 11120375 1.2798731 45.052227 6
TGCAA 8431795 1.2792099 10.491535 9
GTGTT 7935290 1.2763729 20.26999 8
CGCCT 3003265 1.2661045 99.14407 1
CACCT 4192990 1.2648306 17.097672 4
TCACA 6960395 1.25955 6.670864 9
TCCAT 6189440 1.2595321 20.528116 2
CTGTG 5279710 1.2588545 19.682642 2
CGAAC 5578930 1.2546524 41.391724 3
CTTGT 6507480 1.2484965 12.502972 5
ACATG 8213000 1.2460159 15.428788 2
CCATG 4918440 1.2438736 25.51869 3
ACACA 7712980 1.2411587 5.96502 2
GAGCG 4690640 1.2359823 5.0950427 140-144
ACTGT 7176880 1.2244296 16.354097 1
ACGTT 7131875 1.2167513 6.075775 3
TATGG 8479895 1.212911 5.276603 7
GGTTC 5068060 1.2083902 10.930715 3
CTACC 4000240 1.2066867 21.048944 3
ACGTG 5640765 1.1959888 26.966356 6
TTGTT 9239500 1.1958379 24.153023 8
GATAT 11641655 1.1914774 40.325752 4
TTCAC 5851150 1.1906911 11.538293 2
CCTAA 6557140 1.1865771 62.22046 3
GTCAG 5577240 1.1825199 7.0326276 8
TTATG 10259810 1.1808285 22.324133 6
CGTGT 4909730 1.1706393 16.828829 7
ATCCT 5725305 1.1650819 7.037638 2
CATTG 6815485 1.1627729 7.966291 6
ATCGA 7469140 1.133163 14.128737 1
GCTTA 6631365 1.1313608 7.443165 6
TGACG 5325875 1.129224 5.399409 9
TCTTT 7312925 1.1289499 9.284439 9
GATAA 12400780 1.1286094 7.5961723 2
CCTAG 4435705 1.1217898 8.74617 4
GCGTC 3161415 1.1173717 7.2312703 145-146
CAGGA 5924300 1.1169913 18.872625 2
TCAGG 5267465 1.1168395 9.557642 1
CCATC 3690615 1.1132873 5.273534 6
CCACA 4141385 1.1109072 5.8542404 2
ACCTG 4383150 1.1084987 22.551018 6
TGTTC 5777700 1.108484 16.759624 9
TAACG 7301785 1.1077733 12.677502 4
TTAAA 15096970 1.1055886 6.453749 8
AATTC 9045925 1.1042917 10.2351885 1
TGGAT 7706850 1.1023394 14.068353 5
TGGCA 5197830 1.1020751 9.211325 9
ATCGC 4351230 1.1004261 5.6708817 1
CGTAT 6448500 1.1001625 49.267117 3
ATTAT 13356990 1.09999 31.571346 5
GACAA 8125995 1.0962813 7.978356 3
CTCTA 5380515 1.0949183 8.607219 3
CTGCC 2577720 1.086705 7.0475483 8
GTTCG 4547830 1.0843505 18.034782 4
CAACG 4815470 1.0829569 15.719272 1
AGTCA 7133650 1.0822649 5.5227737 7
ACACT 5969410 1.0802218 8.47075 3
GATCG 5073425 1.075698 6.4432716 145-146
TTCTG 5592535 1.0729592 18.65595 2
ATCCA 5925605 1.0722948 18.248724 1
ACGAA 7927530 1.0695063 12.241101 2
TCCAC 3533795 1.065982 6.5783286 1
ATTCA 8681740 1.0598333 9.580376 7
AACGC 4706260 1.0583966 5.639636 1
TCGAC 4182130 1.0576607 17.256428 1
GACTC 4164700 1.0532527 11.3033285 4
TTTCT 6731115 1.0391315 14.025978 7
CCGGA 3294155 1.0353421 5.0313497 80-84
TGTCT 5394570 1.0349785 26.073092 5
TGTCA 6050090 1.0321908 18.658222 9
TATTT 11080230 1.0261368 71.9318 5
ATTCT 7418025 1.0183462 11.809944 7
TACTG 5946175 1.0144621 5.573638 4
ACGTC 3981175 1.006839 13.159883 6
ACGTA 6624100 1.0049597 8.220152 6
TGTAG 7008980 1.0025204 6.188953 4
CACTC 3302660 0.99625933 14.124172 4
TCGAA 6561105 0.9954025 14.151464 2
TACCA 5496560 0.994655 12.624866 4
CGATT 5807370 0.990781 66.00186 3
TTTGC 5162695 0.990492 15.49782 7
GTAGT 6924300 0.99040836 9.731324 5
TCTTA 7197780 0.988111 6.074211 9
TATCT 7197415 0.98806083 7.0479164 7
GATTA 9636640 0.986272 39.59479 4
TGTTA 8506965 0.9790887 8.83042 9
CTCTG 3388615 0.96371233 12.728787 6
GTTGT 5936090 0.9548063 7.929737 7
TGGCG 3191205 0.9456081 6.93436 9
TCTGA 5491380 0.93687075 7.9204855 7
TTACG 5473715 0.93385684 18.520805 4
ACTCT 4588930 0.9338331 18.060492 5
CATGA 6146110 0.93244255 15.42703 3
TTTGT 7128125 0.9225695 24.706482 7
CCTCT 2718150 0.92205817 5.6079903 7
TGTGA 6415575 0.9176435 15.921001 9
GGCGT 3091895 0.91618085 85.38075 1
TTCCG 3216760 0.91483736 23.860252 3
GATGT 6366840 0.9106728 18.131489 3
GGTCT 3818895 0.9105487 8.049815 7
CTATT 6626105 0.90963143 32.26154 4
GTACT 5258845 0.89719844 6.13899 5
TGTCC 3152005 0.8964211 14.226084 9
ATGTC 5227960 0.8919293 14.197388 4
ACCAT 4899430 0.88659865 14.651176 5
TCAGA 5807430 0.88106054 5.021023 9
CGGTC 2490585 0.8802733 7.6710935 6
ACCTC 2918050 0.8802403 11.837891 6
ACTCG 3479510 0.87996805 6.763328 3
GTGGA 4942575 0.87858266 8.275222 8
CTAGC 3466700 0.8767284 5.449427 2
CTGTA 5136025 0.8762445 7.3774805 3
CATCT 4293675 0.8737496 9.867096 5
TCTGC 3066170 0.87200993 27.672134 3
CGACT 3443380 0.87083083 34.008274 2
CTGAC 3414975 0.86364716 5.494633 1
AGACT 5688550 0.86302507 6.7833 3
AGTCT 5054025 0.8622546 6.363575 5
TGTCG 3613615 0.86160326 12.928156 3
ACCTA 4756460 0.86072683 7.3091536 6
TTATT 9239890 0.8557034 5.96079 8
CTAAT 7006980 0.8553852 41.98419 4
TATTC 6209520 0.85244274 12.631351 6
GGACA 4508620 0.85007334 21.97747 2
ACAGG 4499310 0.848318 10.373633 1
GATCT 4967930 0.8475661 7.545024 5
TCGCA 3342055 0.8452057 5.9717665 9
CCATT 4143920 0.843275 19.464987 5
GTTCC 2962305 0.84247094 21.966347 2
AGGAC 4462205 0.84132206 21.961369 1
AGCGC 2665750 0.8378364 9.276407 4
CCTAT 4083110 0.8309004 47.870018 3
CCTGC 1967800 0.8295773 7.1558547 7
ATATT 10058435 0.82834363 31.756338 5
TCACC 2729195 0.82327145 17.099415 3
GGATA 6407575 0.8149966 10.595136 1
ATTAA 11053040 0.80944157 6.26598 7
CTAGG 3805020 0.80676323 11.211421 1
GTGCA 3786675 0.80287355 8.244191 8
GTGTG 3996445 0.79887635 13.464461 8
CGATA 5262315 0.7983598 59.783466 3
TGAGT 5532175 0.79128754 30.39957 5
TGTGG 3958470 0.79128534 17.933231 9
ATGGA 6183450 0.7864896 5.7739596 4
ATGTT 6816005 0.78447187 9.464431 8
AATTA 10706605 0.7840713 5.6358423 6
TGTTT 6053400 0.78347135 20.426023 9
CCGGT 2215470 0.78303665 29.148165 5
TATGC 4582165 0.7817518 7.224506 7
CCTGG 2209150 0.7808029 5.3591003 7
GAATT 7625490 0.7804388 12.115711 2
CACAC 2907665 0.77996755 9.935533 3
TCCTA 3825105 0.7783971 7.0354285 3
GGATG 4371115 0.777001 11.126807 6
ATTCC 3808750 0.7750689 17.056715 2
ATTGT 6667650 0.7673973 17.670086 7
TAGAA 8367000 0.76149064 6.1979556 9
GTGTC 3144665 0.7497904 15.864272 8
GCATC 2961405 0.74893934 7.8429666 2
GACAC 3321590 0.74699646 10.52948 2
TCTCT 3263740 0.74687815 10.449276 7
AGTGG 4163820 0.74015266 6.7473516 7
GTAGG 4124530 0.73316854 5.490786 3
GACAT 4812275 0.73008305 15.395742 1
ATTTC 5301670 0.7278131 24.547997 6
GATCC 2876455 0.7274554 8.743974 1
AACGA 5384915 0.7264811 6.057041 2
GTCAT 4243495 0.7239721 7.726396 3
TCTGG 3032980 0.72316104 8.836818 7
GTGTA 5048665 0.7221293 6.989511 8
GCAAG 3803170 0.71706504 6.8384356 3
CTGGA 3359800 0.71236503 5.8382487 8
CTACT 3486865 0.7095663 5.454361 1
ATAAC 6391455 0.6938318 9.076025 3
GGATT 4835105 0.69158304 7.0871224 5
GGAAT 5341870 0.6794467 15.032842 1
TACTC 3332615 0.6781769 5.4386034 2
TAATT 8151025 0.67126244 27.727585 5
ATTAC 5426240 0.6624145 13.252163 3
AGTGA 5203120 0.6617987 6.4805164 7
GGACT 3109035 0.65919626 9.575286 4
CTAGT 3860690 0.6586627 12.819627 5
CTGTC 2309845 0.6569133 11.186827 8
ATGAG 5138300 0.65355414 21.983536 4
CCTGT 2276000 0.64728785 12.298224 2
ACGCC 1708920 0.6406506 9.411668 2
CGTCT 2248100 0.63935316 8.60571 7
CGCCA 1703520 0.6386263 9.4046135 3
TTAGC 3691720 0.6298352 5.2328715 8
TTGTG 3907375 0.6284923 15.782766 8
GCGCT 1774635 0.62722766 9.992552 5
TAGCA 4122025 0.6253634 5.622905 9
GTCTC 2160295 0.6143817 10.702957 6
TGTGC 2563410 0.61120033 8.302257 7
TATTA 7409360 0.610184 6.9382586 6
GCCAT 2410335 0.6095737 6.350984 4
CTGAG 2873740 0.60930765 6.3927827 1
TCTGT 3157600 0.60580325 18.742031 7
ACGAT 3972045 0.6026094 6.8133826 3
TTGTA 5225430 0.6014085 8.151183 8
GGATC 2836470 0.6014054 6.0269794 6
CACAT 3315650 0.59999853 6.6378627 5
ATCTG 3469970 0.5920029 7.5613213 6
GAGTG 3323540 0.5907861 20.753532 6
GCGTA 2743560 0.58170605 61.19275 2
AGTGC 2710350 0.57466465 8.16164 7
GTCTG 2394070 0.57082415 19.993574 6
TACGA 3751290 0.5691181 13.751381 1
AGTGT 3963215 0.5668734 10.989921 7
TACGT 3310925 0.5648686 28.326866 5
TGTAA 5480775 0.5609357 9.591674 9
ACGAC 2486155 0.5591144 14.922932 1
AGACA 4131375 0.55736554 6.3208447 1
GCATG 2615760 0.5546092 9.546288 2
TTTAA 6726675 0.55396277 5.3187475 7
TGTGT 3374115 0.5427186 18.280428 5
TAGGT 3752940 0.536797 6.62598 1
GTCGA 2487015 0.5273119 6.3848033 4
TCTCG 1846600 0.5251677 6.3699503 9
CGTAG 2475485 0.5248672 6.711478 2
TGAGC 2472115 0.52415264 5.432552 9
ATTTA 6342370 0.5223141 13.037678 6
CAGAG 2748485 0.5182104 5.0256042 4
CATGT 3032615 0.51738685 12.55899 5
CGATC 1998360 0.5053853 11.357319 4
TAGTG 3414155 0.4883393 10.014843 6
GAGTA 3717230 0.47280443 7.980523 6
TATGT 4043870 0.4654196 12.053363 7
TAGTC 2723815 0.46470332 5.1510134 6
TCCGG 1280665 0.45263878 29.656143 4
GAGAC 2400380 0.4525773 8.430633 2
TGAGA 3461320 0.44025454 7.8328805 1
TTCGC 1510010 0.4294425 11.9469 1
GTGAG 2407180 0.4278957 7.93175 4
AGCGG 1606355 0.42327407 6.147689 4
CTCTC 1228960 0.41689113 6.9196477 8
ATGTG 2796965 0.40006033 6.230358 4
TCGCC 915145 0.3858032 17.736332 2
GTCTA 2250355 0.3839275 6.5249457 6
AGCAT 2509720 0.38075632 11.513155 1
CGCGT 1060260 0.37473872 7.9094815 3
TTATA 4532255 0.3732454 6.330897 6
TAGCG 1716395 0.3639204 6.259749 3
TCTAG 2093900 0.35723504 7.219073 4
TCGCG 973645 0.34412548 7.902832 2
GAGTC 1595905 0.33837333 10.934305 6
TGTAC 1902695 0.32461405 5.6871033 9
GTACG 1396260 0.29604343 12.770064 4
TGCGA 1302975 0.2762646 5.2194242 9