FastQCFastQC Report
Tue 9 Jul 2019
SL_C_1_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SL_C_1_R1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 38627814
Filtered Sequences 0
Sequence length 100-150
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GACATGAGTTCAGTCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 47318 0.12249722440933364 Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 55516450 28.71866 72.98061 145-146
CACCA 15205015 3.8150547 6.289111 105-109
ACCAA 17959675 3.6350188 5.9231696 85-89
CAAGA 14742465 3.1644747 4.548071 30-34
CTTCA 21013695 3.06525 4.41514 70-74
AGAGC 10132760 2.8594897 9.894628 25-29
CCAAG 10673315 2.8401198 11.113555 1
TCAAG 15446875 2.814815 7.3000503 9
CTGCT 14555420 2.79137 18.75377 4
GCTTC 13843715 2.6548827 7.9196944 6
TCACC 12431930 2.6480742 10.060466 3
TTCAA 21939305 2.5815504 5.2480545 8
GAAGA 11258080 2.562827 7.2737136 3
CTTGA 16386485 2.5349696 6.40624 80-84
TTCTT 29761700 2.523892 7.851923 8
AAGAG 10966340 2.4964142 7.685768 25-29
TACCA 14327315 2.4617877 11.860021 4
TCTTC 19804365 2.4524608 9.194773 9
CTTCT 19644930 2.432717 6.799624 5
GTCAA 13279845 2.4199266 10.705002 8
AGCAA 11002690 2.3617313 9.398339 2
GCTTG 11312125 2.3007014 21.680925 6
ACCTT 15628610 2.2797325 6.7644596 5
GGAAG 7562110 2.2632217 8.699263 20-24
ACTGC 10006420 2.260444 8.160764 50-54
TAGCA 12246085 2.2315493 8.006746 1
ACGAC 8127995 2.162822 23.42191 1
GAGCA 7550665 2.130816 8.791027 25-29
TGCTT 15830900 2.079075 24.783998 5
TTGAA 16303205 2.0344834 5.4557395 8
AGCAT 10984940 2.0017366 11.917809 1
CAAGG 6988420 1.9721491 12.359131 4
CTGCA 8648860 1.953772 6.534471 8
AGCAC 7306910 1.944335 7.911552 25-29
TCTTG 14774450 1.9403312 9.640728 9
ACCAT 11292460 1.9403247 14.651156 5
TGTTG 13893955 1.9351465 25.58872 9
TGAAG 9984610 1.9295844 5.3969297 9
AGTCA 10583115 1.9285139 6.5107355 7
GGCAA 6802380 1.9196482 5.057995 40-44
CGATA 10493365 1.9121588 47.690098 3
TCAAA 13637475 1.8902273 7.755737 9
GAAGG 6279505 1.8793583 13.256799 3
GTCAC 8236975 1.8607274 8.791647 8
GTTGT 13259335 1.8467568 9.727313 5
CAACT 10674015 1.8340602 12.003995 5
GATAA 12432430 1.827509 11.399604 2
TTCTG 13777375 1.8093852 12.826466 2
ACGTC 7998515 1.8068595 26.309668 1
GTGTT 12886630 1.7948465 28.47989 8
TCTGC 9348465 1.7928046 18.746689 3
CACCT 8329140 1.7741557 10.061392 4
CCAGT 7834695 1.7698526 6.8176827 40-44
AACTG 9662015 1.7606659 11.854991 2
CAGTC 7733590 1.7470131 6.5048633 45-49
TTCAC 11939505 1.7416058 6.8856287 2
TCAGC 7691895 1.7375941 5.4377055 9
AGAAG 7629565 1.7368199 12.4848385 2
CCGAT 7687745 1.7366567 120.833916 2
CAACG 6518435 1.7345253 20.458035 1
CATCT 11869030 1.7313254 6.5372267 5
AAGGT 8917995 1.7234546 10.5779 1
TTTCT 20263255 1.718392 5.5617967 7
ATCTT 16905890 1.6887814 5.9737134 8
GCAAG 5979505 1.687431 12.349118 3
TGGCA 7012985 1.6801238 21.007784 9
TTGTT 18655535 1.6778154 21.177135 8
CTGAA 9104155 1.6590093 6.1150384 35-39
CGTTC 8643220 1.657556 10.536861 1
TCTTT 19538955 1.656969 12.175955 9
TCACG 7224885 1.6320969 7.4782767 9
CGAAC 6126875 1.6303332 44.106186 3
ACTTC 11114235 1.6212243 8.130493 4
CCATG 7138395 1.6125588 25.94844 3
TGAAC 8843850 1.6115754 5.7571845 35-39
TTGTG 11392680 1.5867696 9.083876 6
CTTGT 11985900 1.5741105 11.439464 5
ACCTG 6854745 1.5484825 20.036137 6
GATGA 8012180 1.5484006 12.315622 3
TGTTT 17194780 1.54644 23.96393 9
CGGAA 5455615 1.5395879 7.697559 20-24
CACGT 6807840 1.5378867 6.880708 30-34
AGGTT 9353815 1.5346137 15.691116 1
GGTTT 10990055 1.530692 5.7406034 5
TTCCA 10481085 1.528867 8.020309 3
CTGTG 7505895 1.5265765 21.505646 2
ACGTT 9855515 1.5246361 9.019578 3
AGGAC 5390250 1.5211416 41.66008 3
TACTG 9829635 1.5206325 9.900283 4
TGTGT 10896915 1.5177195 25.769976 5
ACGAA 6983375 1.498984 20.382137 2
TGTCA 9610520 1.4867359 25.991838 9
TGAAA 10083250 1.4821906 7.5178027 9
GCCGA 4227505 1.4789402 187.21152 1
CGAAG 5230685 1.4761121 12.407774 2
ATAGC 8078105 1.4720368 5.860388 2
CTTCG 7675455 1.4719626 5.9691796 1
CTTGG 7219230 1.4682735 16.23418 3
TGCAG 6056365 1.4509432 12.367909 9
ACACG 5439270 1.4473644 7.497454 30-34
ATAAC 10434240 1.4462417 10.7528715 3
CGTCA 6357685 1.436197 31.868599 2
ATTTC 14302605 1.4287311 11.795506 6
GGTCA 5957770 1.4273225 7.429175 7
GAACT 7814370 1.4239777 6.1055107 35-39
AACAG 6608790 1.4185789 5.1981707 2
GAACC 5286060 1.4065962 44.08364 4
AACCT 8180130 1.405549 28.110447 5
AACGA 6523540 1.4002799 7.995069 2
GTTCA 8996535 1.3917532 7.3011904 1
GACAA 6346065 1.3621851 10.939338 3
ACTGT 8757390 1.3547575 18.797855 1
TGAGA 7008550 1.3544433 32.537624 1
TGTGA 8232600 1.350664 17.34706 3
TTGCT 10281285 1.3502431 12.408359 4
GACTT 8717675 1.3486137 22.20976 3
TCGCC 4771065 1.3360912 21.335064 2
AGCTA 7271885 1.325123 12.632292 1
GTTGC 6477605 1.317439 19.21583 3
TGGAA 6802840 1.3146886 7.077408 9
AGATC 7203980 1.3127491 5.1399794 15-19
CACTG 5791240 1.3082374 9.387966 2
TGCTG 6411090 1.303911 14.288593 9
CACAC 5189535 1.3020942 8.36122 3
ATCCA 7541350 1.2957908 19.727213 1
ATTTG 12177425 1.2900728 32.57589 6
CGCCT 4587605 1.2847148 45.82197 1
AATTG 10218930 1.2752242 14.2463045 3
TGACG 5308360 1.2717413 6.2731347 9
TACGA 6964375 1.2690868 17.293865 1
TTGGA 7673820 1.258989 13.071812 4
CCGTC 4470940 1.252044 8.599926 70-74
TTGTC 9527215 1.2512109 16.118917 8
TGACT 8067975 1.248106 16.19529 5
CGTCT 6494075 1.245403 8.945087 7
CCTTG 6486645 1.2439781 5.037949 6
GTCTG 6113035 1.2432915 16.84633 6
GCCGT 4169615 1.2383406 8.952849 65-69
GTGAT 7546420 1.2380873 23.105967 1
ACTGA 6766975 1.2331156 5.889685 4
GCTAC 5430775 1.2268085 15.594095 2
CATGT 7873885 1.2180804 22.957367 5
GGATA 6287535 1.2151029 14.971888 1
TTGGT 8684480 1.2095722 22.432251 5
CGATT 7793715 1.2056782 42.329514 3
TTTGC 9170905 1.2044166 10.679145 7
ATCTG 7779800 1.2035255 6.6687536 6
AACGT 6513455 1.1869177 13.969939 5
CTGTT 9035080 1.1865788 15.264916 8
TTACG 7643115 1.1823806 15.62436 4
TTTGT 13122870 1.1802263 16.272738 7
GTAGC 4923970 1.1796519 5.653037 8
TCCGG 3955405 1.174722 25.983112 4
ACTGG 4898330 1.173509 15.591225 3
GTCTT 8934405 1.1733571 17.385319 8
CCATT 8012660 1.1688001 10.931332 5
CTGAC 5168615 1.1675868 7.3143487 1
GATCG 4868185 1.1662871 6.333738 15-19
TGAGT 7100465 1.1649228 34.376053 5
CTGCG 3910765 1.1614643 8.839447 50-54
ATCGG 4820225 1.1547971 6.692881 145-146
TCTGA 7458555 1.1538295 5.0630054 35-39
TTTGG 8277150 1.1528393 6.4250927 7
AGGAT 5952920 1.1504365 6.4323387 5
AGTGT 7002855 1.1489086 19.83555 5
TCCAG 5084685 1.148627 6.73248 40-44
AGTGA 5935515 1.147073 7.7209344 3
TGCAA 6266445 1.1419061 8.6491785 9
TACGT 7370855 1.1402622 23.688282 5
TCGAT 7366365 1.1395677 5.836672 5
GTGAC 4735050 1.1343914 25.331793 4
TCAGG 4729780 1.1331289 11.688647 1
GAGTG 4444040 1.129119 30.879015 4
TATCA 9562455 1.1251932 6.470316 9
GAGAA 4939945 1.1245456 12.415925 1
TGGAG 4414135 1.1215209 8.129671 9
CTAGG 4669205 1.1186168 16.56509 1
CAGGA 3960165 1.1175681 41.643936 2
GAGTT 6804475 1.1163617 6.229419 6
AAGGG 3717135 1.1124806 7.745656 85-89
ACGAT 6098275 1.1112615 6.78497 3
GCACA 4172350 1.110243 7.0442286 25-29
CGCCA 3361225 1.1087679 7.2889543 3
TCTGT 8433140 1.1075258 15.427651 7
TTATC 11030435 1.1018641 6.182643 6
TTCGT 8306940 1.090952 14.611412 5
TAAGC 5980985 1.0898881 5.166226 2
TTGCA 7041670 1.0893378 9.410579 8
TGCCA 4796375 1.083498 20.394423 9
GTTCG 5325400 1.0830994 22.933062 4
GCCTA 4792720 1.0826724 84.81939 2
TGGAT 6581345 1.0797544 21.469866 5
GTGGC 3418815 1.0768201 15.195388 8
GACTG 4490790 1.0758733 10.02112 6
GAACA 4996190 1.072434 5.187756 1
GGTTG 4969475 1.0718894 20.375547 2
AGGGG 2715895 1.0686274 10.956987 85-89
GTTCT 8114290 1.0656512 12.824183 1
AAAGG 4679340 1.0652206 6.012947 85-89
ATTGA 8521915 1.0634531 5.1256 7
GGACA 3764105 1.0622395 29.257826 2
CAGTA 5796595 1.0562875 8.494111 1
CGCTG 3553635 1.0553997 5.048902 6
GAGAT 5444950 1.0522684 8.310858 5
TAGGT 6326470 1.0379388 11.444266 1
TGCGA 4326345 1.0364767 7.33861 9
GTTGG 4788195 1.0327883 10.829947 4
TGATG 6272890 1.0291485 37.538586 2
GAATT 8243550 1.0287158 14.240703 2
GAAAA 5934910 1.0276376 5.076326 80-84
CGTTG 5046915 1.02646 14.365034 4
GTCCA 4534495 1.0243396 6.4842463 8
GTTCC 5337210 1.023545 10.535334 2
GTCAG 4258955 1.0203319 5.7961755 8
TGTGG 4709210 1.0157516 7.3764887 9
TCTGG 4991290 1.0151469 8.481716 9
GGTGT 4690335 1.0116804 23.178867 7
GAGTC 4213670 1.0094827 19.109495 6
CTGAT 6520280 1.0086794 13.609752 1
CCTGC 3595270 1.006821 5.9666295 7
TGTTA 9471925 1.003453 9.545241 9
ACAAC 4954440 1.0027732 10.312761 4
GACAT 5491470 1.000686 20.236929 1
GTCAT 6455060 0.99858993 18.730186 3
ACGTG 4158925 0.99636734 29.599417 6
TGTTC 7586185 0.9962951 16.98272 9
ACTTG 6412565 0.992016 13.586449 4
TGCCG 3332385 0.9896903 8.106949 65-69
GATTA 7914810 0.98769224 34.13064 4
GTATT 9313990 0.98672146 40.144367 4
CTAGC 4358690 0.9846253 5.5605674 2
TAGAG 5077205 0.9811995 6.5927305 2
TCCAT 6705790 0.978168 16.749783 2
AGAGT 5053700 0.9766571 23.50444 3
CGGTA 4073165 0.9758215 43.720387 2
TTCCG 5087490 0.97565484 16.785202 3
GTCGA 4068955 0.9748129 9.174778 4
AGTGG 3826995 0.97234344 8.385986 7
CATCG 4302635 0.9719625 5.248584 1
GGTTC 4776675 0.9714976 14.00608 3
GTGAA 5026760 0.97145075 7.7766366 8
ACTCC 4511985 0.9610793 6.1146936 40-44
CTACC 4510585 0.9607811 14.704197 3
CATGG 4006335 0.95981085 27.517916 4
TGGTT 6873840 0.9573867 8.9327755 5
TCGGA 3950895 0.9465289 6.286212 20-24
AGTAC 5193020 0.94630086 8.500975 2
GGATG 3702810 0.9407911 17.59852 6
CTGGC 3156980 0.9375965 7.7613363 8
GGAGT 3670175 0.93249935 7.0122123 2
GTCCG 3104880 0.92212325 5.00739 8
ATTTA 11433825 0.92134255 6.495694 6
CAGGT 3838085 0.9195026 5.841808 4
TGGTG 4257100 0.9182339 28.29314 6
CATTG 5934535 0.9180653 6.376007 6
CAGAG 3248480 0.91672903 10.754385 4
CTCCA 4302160 0.91638535 5.8786116 40-44
AGACA 4265940 0.91568553 9.14775 1
TATTT 13383620 0.91554743 36.508392 5
AGAGG 3052665 0.91361517 10.4531975 1
TTCGC 4739290 0.90887874 14.604832 1
CTCTT 7251785 0.89802 6.362511 8
ACATC 5172700 0.8887981 7.237041 6
GTGGA 3497785 0.8886994 8.337225 8
ATGGT 5405810 0.8868927 18.367216 5
ATTGG 5372700 0.8814606 18.726992 4
AGGAG 2941690 0.880402 10.431955 3
CGACT 3894265 0.879712 32.444305 2
CGCGT 2955015 0.8776146 9.012701 3
AATTC 7447950 0.8763841 10.305907 1
CTGTA 5654075 0.8746786 8.508476 3
GTGTG 4053320 0.87427956 19.051992 6
TTGGC 4275090 0.8694834 11.144767 8
CCTAA 5038860 0.8658011 36.40832 3
TGGTC 4250640 0.8645107 14.895582 6
TCGTT 6570380 0.86288923 12.468183 2
CTGTC 4471830 0.8575865 11.254762 8
GCGCT 2881825 0.85587776 9.281557 5
GTGTC 4197775 0.8537588 24.740932 6
CCACA 3384460 0.84918696 5.198733 2
GAGGA 2825575 0.8456507 10.473138 2
GCGGT 2682225 0.8448172 57.47473 1
ATGAG 4344275 0.8395565 33.762535 4
CCTGT 4354905 0.8351632 10.562277 2
ATGCC 3678660 0.8310068 6.3290043 65-69
GTGTA 5062790 0.8306159 6.770152 6
CTGCC 2963410 0.82987463 7.5223746 8
GGATC 3452395 0.8271016 12.382481 6
GGCGT 2622725 0.82607657 61.937817 1
GGAAT 4270155 0.8252324 22.048874 1
ATGTT 7782065 0.82442963 12.0144825 8
GATAT 6595930 0.8231087 32.634033 4
ATTGT 7742815 0.82027155 14.840568 7
AGACT 4484545 0.81719863 8.547428 3
GCGTA 3398820 0.81426644 47.118942 2
AGGTA 4154255 0.80283403 10.942211 3
TCATG 5158640 0.7980353 14.9254875 4
GATGT 4861775 0.79763687 27.090948 3
GGTAC 3314000 0.79394585 13.552403 4
TAATT 9739075 0.7847789 16.77878 5
TCGTC 4082980 0.7830147 7.487759 6
TCGAA 4281545 0.78020674 12.90613 2
CATGA 4280515 0.78001904 20.241282 3
CACAG 2916310 0.77601665 10.148851 2
TGTCC 4036220 0.7740473 12.870566 9
GGCCT 2595050 0.770708 62.888447 1
TGTCT 5790010 0.7604031 17.771753 7
GGTAT 4621305 0.75818455 29.940992 3
AGTCT 4900760 0.7581417 9.627479 7
ATTCC 5193875 0.7576262 12.773378 2
GACAC 2820160 0.75043154 11.298942 2
AAGCG 2657865 0.75005597 8.004526 3
ATTGC 4836360 0.748179 11.613068 7
TGTAG 4550665 0.74659526 9.019108 4
ATTAC 6337445 0.74571335 11.888547 3
GTGCA 3106275 0.7441805 12.045191 8
TGTGC 3646120 0.74156123 10.424804 9
GGATT 4502385 0.7386742 5.1076746 5
CGGTC 2485660 0.7382201 8.17473 6
GATCC 3257250 0.73581076 7.0658426 1
ATGTC 4742010 0.7335831 11.953767 4
GTACG 3056030 0.73214316 13.103381 4
ACAGG 2576620 0.72712845 27.876663 1
TGCAC 3217615 0.7268572 9.491854 9
ATTCT 7228000 0.7220271 5.9539633 7
TGGTA 4389080 0.7200851 7.167949 6
CGTGT 3525890 0.71710837 17.158735 7
CCGGT 2408850 0.7154082 25.365818 5
GAGTA 3697740 0.71460986 8.280626 6
ACCTC 3333070 0.7099635 8.060621 6
AGTGC 2924935 0.7007364 11.647699 7
ATGTG 4266655 0.6999996 14.419543 4
ATGGA 3607605 0.6971907 10.288747 4
AGTAG 3588040 0.69340974 5.307215 4
CCTAG 3066465 0.6927125 7.0552077 4
ACATG 3767755 0.6865812 20.254877 2
GCATG 2860245 0.6852383 12.777545 2
TGTCG 3357930 0.6829481 12.172583 9
CTGGT 3347485 0.68082374 13.235444 4
ATCGA 3734345 0.68049294 12.9277725 1
TGTAA 5445820 0.679586 6.3729544 9
TGGGA 2626725 0.6673849 6.168923 9
GCGAA 2358835 0.66566896 7.593976 50-54
TGGCT 3260775 0.66318834 5.8481183 9
GTGCT 3233530 0.65764713 11.405181 8
TTGCC 3419780 0.6558293 11.280456 8
ATCGC 2861375 0.6463828 6.8673964 1
ATATT 7949700 0.6405903 20.58105 5
ACAGA 2976430 0.63889164 8.181767 3
TCGAC 2816700 0.6362907 12.604978 1
TAGCG 2645445 0.6337779 7.710422 3
GATGC 2640780 0.63266045 5.9242206 7
CGTAT 4087390 0.63231426 30.420784 3
GTACT 4086175 0.63212633 9.907679 3
ATCGT 3977340 0.6152896 7.8233833 1
GTGAG 2418990 0.61460465 10.136049 4
GCTCT 3172020 0.60831505 7.0155463 2
CTTGC 3150080 0.6041075 7.56984 7
TGGCG 1916270 0.6035653 8.835846 9
ACTCT 4127545 0.60208154 12.752779 5
CATGC 2664985 0.6020184 5.2834296 7
TCTCC 3284110 0.5938639 6.361591 9
AGCTC 2624235 0.592813 8.273039 1
GGTGC 1861275 0.58624357 14.571066 7
GAGAC 2066520 0.583177 13.232015 2
TAACG 3189750 0.5812538 14.130414 4
AGCGC 1657140 0.5797299 11.25182 4
GGAGA 1935540 0.57927704 10.397277 4
GGTGA 2231840 0.5670545 6.8289433 7
CGATC 2498005 0.56429774 8.405227 4
TATTC 5638805 0.5632775 6.3274746 6
TATTG 5260110 0.5572545 14.371716 6
TTGCG 2730585 0.5553564 8.166064 8
GTAGT 3353870 0.5502456 13.260731 5
ATTAT 6816345 0.5492641 21.81029 5
GTGGG 1634010 0.5458152 6.1907587 8
GAGAG 1822045 0.5453097 36.397186 2
GTCGC 1824780 0.5419443 5.5311217 8
CCTAT 3693685 0.53879476 23.880814 3
CTCTG 2775260 0.53222626 9.445079 6
ATGGC 2200155 0.5270983 5.586585 8
TGCGC 1763685 0.5237996 7.270301 9
GTGCC 1761700 0.52321005 13.678105 8
GTGCG 1633055 0.5143614 10.381021 8
ACGCC 1516400 0.5002151 7.3018546 2
TTCTC 4015130 0.49721107 5.833655 8
CTAAT 4180945 0.4919627 24.926579 4
TCTCA 3328540 0.48553133 12.973464 9
GATCT 3104445 0.4802539 6.8871093 7
ACACT 2758970 0.4740594 9.407231 1
GTCTC 2461790 0.4721105 14.869963 8
GGACT 1967775 0.4714263 11.714846 4
GGTCT 2263150 0.46028772 11.064935 7
GTCGG 1425295 0.44892347 6.0032454 8
CTAGA 2427335 0.44232237 6.233203 1
CACTC 2073345 0.44163468 9.039873 4
ACGTA 2403985 0.4380674 6.453593 6
CACAT 2546285 0.4375149 5.6514573 5
TAGTG 2660395 0.436472 11.699601 6
GACTC 1850950 0.41812843 10.587475 4
TCTCG 2177120 0.4175178 7.0370755 9
AACGC 1550020 0.41245311 5.8861175 1
CGTGG 1305895 0.4113162 7.6241536 7
CGTGC 1374405 0.40818673 11.717176 7
TAGTC 2624670 0.40603322 6.1104846 6
GGTGG 1200345 0.4009563 9.836849 7
TATGC 2542080 0.3932567 6.6885834 7
ACACA 1918615 0.38832557 7.8409886 1
AGCGG 1046125 0.38812667 10.494863 4
TCTCT 3058375 0.37873185 5.7515054 7
TGCGG 1196985 0.37701294 9.287493 9
TGCCT 1925670 0.36929592 5.2151694 9
TCTAG 2337690 0.3616378 5.652711 4
CTATT 3402550 0.3398912 16.34289 4
CGGTG 1050650 0.33092198 18.954319 6
TCGTG 1368050 0.27823904 9.138346 6
TCGCG 923625 0.27430886 9.0124855 2
ACTCG 1154465 0.2607929 5.7577043 3
TTATG 2453665 0.25994056 16.294544 6
CTCTA 1726740 0.2518781 5.3366256 3
CTAGT 1373980 0.21255304 10.183619 5
TATGT 1922650 0.20368499 10.903077 7
TGGGG 463650 0.15487497 5.076758 9