FastQCFastQC Report
Tue 9 Jul 2019
SL_C_3_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SL_C_3_R2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 39486251
Filtered Sequences 0
Sequence length 100-150
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGGTG 17117275 3.3507807 22.221947 6
TTGGT 19736030 3.1785827 24.907036 5
TGCTG 13128305 3.0770466 12.623038 9
TGAAG 23547310 3.07106 8.698834 9
TCTTG 15120255 2.915727 28.292116 9
GCTGC 8250670 2.8142736 5.190969 20-24
TGGCT 11350185 2.6602862 12.741521 9
CTTGG 11267555 2.6409187 14.2029705 3
TGATG 18150415 2.630552 31.220816 2
TTGAA 24262255 2.6033976 10.966094 8
CCTTC 7649265 2.570237 5.3334103 65-69
GTCGT 10807670 2.5331302 6.099556 4
TTGCT 13131760 2.532274 21.42681 4
TCAAG 15995890 2.4978676 6.9827356 9
CGTCG 7193580 2.4537036 7.480885 145-146
TCGTG 10422760 2.4429135 9.096024 6
AAGGT 18048550 2.3539066 8.573012 1
CTGGT 9972545 2.337391 17.547258 4
TTCAA 18123095 2.3283901 10.76337 8
GCCGA 7555140 2.319029 210.88109 1
GCTCT 8251165 2.315553 12.018948 2
ATGCT 13154205 2.2826483 7.65448 15-19
TGCTA 13112150 2.2753506 10.114378 9
TTGAC 13064195 2.267029 5.224348 8
GGTGA 12529205 2.2070973 6.7084455 7
CTCTT 9261255 2.1383147 14.253875 8
CTTCA 10206860 2.1207063 5.954152 7
TGAAA 21943955 2.118902 14.333549 9
GTTGC 9011335 2.1121004 26.05223 3
ACTTG 12080575 2.0963414 12.242644 4
TGGTC 8937550 2.0948064 16.402271 6
ACAAC 12376895 2.0824444 10.112557 4
CTGAA 13310890 2.0785866 7.507525 8
TGCCA 8161025 2.060966 10.731287 9
TGGTA 14193230 2.0570347 9.1823845 6
GGGGG 7106750 2.055217 6.8234987 70-74
CTGCT 7318195 2.05373 28.10178 4
TCTTC 8825455 2.0376935 15.523723 9
CAACT 10468570 1.9573226 14.617601 5
CTTCT 8411650 1.942151 16.11365 5
TTGCC 6906895 1.9383057 9.943979 8
GTGGT 9822615 1.9228195 5.891036 8
GTCTT 9928845 1.9146373 18.513979 8
TGGTT 11806220 1.9014487 11.504848 5
TTTGG 11780485 1.8973039 9.912196 7
TTGGC 8054095 1.8877397 11.52771 8
GTCAA 11941020 1.8646722 9.616621 8
GGTGG 7819315 1.8604486 7.842346 7
CCTTG 6608220 1.8544875 7.189937 6
ATGGT 12776345 1.8516845 16.158367 5
ACTGG 8672015 1.8290771 15.788366 3
CCAAG 8009955 1.8202991 9.995307 1
CAGCC 4949350 1.8189714 5.7469974 60-64
TCGTC 6461820 1.8134027 7.10145 1
GAAGG 11311165 1.7930479 8.42397 3
CGTTG 7568205 1.7738557 11.573985 1
TGCCG 5197170 1.7727358 9.425522 9
TCATC 8461055 1.757976 5.259206 4
CTGGC 5142750 1.7541732 7.6191063 8
TGCAG 8260770 1.7423387 6.4307837 9
AATTG 16074035 1.724782 13.325516 3
TTTGA 14432070 1.7208831 7.617229 7
TCAAA 14867155 1.7188495 9.482695 9
TGTTG 10649840 1.7152082 20.140383 9
TTATC 11954290 1.7067132 15.667495 6
CTCAA 9103855 1.70216 7.582748 8
AACGT 10881035 1.699148 13.850282 5
GGTGC 5954120 1.6962138 8.527059 7
GCCTA 6693185 1.6902808 123.061226 2
ATTGC 9723220 1.6872698 8.906616 7
GTTGG 8566305 1.6768913 9.673287 2
GGTAA 12779135 1.6666652 5.5155745 7
AACTG 10579065 1.6519934 11.666201 2
TTCTT 10355860 1.6429954 21.548424 8
AGCTC 6464695 1.6325785 10.812922 1
GTGAA 12464060 1.6255733 10.733575 8
ACCTT 7808025 1.6222942 11.738963 5
TGCTC 5761300 1.6168135 6.218818 9
CGTCA 6385760 1.6126444 15.382745 2
CGAAG 8490965 1.6115942 10.077767 2
GCTTC 5710575 1.6025783 17.3117 6
TTCCA 7680835 1.5958678 14.736175 3
TCAGC 6300125 1.5910183 13.242295 9
GTGAC 7532710 1.5887783 18.105799 4
CTTGC 5650375 1.5856841 6.7939796 7
TTGGA 10911540 1.581417 9.227977 8
TGACT 9095515 1.5783441 14.782659 5
CTCGT 5622870 1.5779654 6.727209 4
GACTG 7465210 1.5745412 7.1647716 6
GATGA 12071025 1.5743134 9.140625 3
GCTGT 6695890 1.5694002 5.5763006 4
ATTGA 14539875 1.5601631 7.600513 7
GGTAC 7325835 1.5451447 14.004801 3
GTGCC 4515890 1.5403539 8.867293 8
TCTCA 7370310 1.531349 20.180948 9
CGTGA 7245560 1.5282134 6.9039407 7
CTTCG 5407325 1.5174762 6.6756597 1
TGCTT 7862870 1.5162433 40.289154 5
CGTGG 5305775 1.5115129 8.428167 7
CCGAT 5982950 1.5109198 173.8863 2
GGTGT 7708230 1.5089194 15.491698 7
TTTCA 10565870 1.5084887 9.557291 7
GGCTC 4407390 1.5033448 5.2597423 10-14
TCAAC 8026535 1.5007321 10.757336 9
TTCGT 7777920 1.4998618 16.314468 5
TTCTC 6437255 1.4862865 16.078085 8
GGTTG 7579090 1.4836395 21.780396 2
ACTTC 7081080 1.471255 14.602444 4
TATTG 12264010 1.462363 16.089285 6
ATCGG 6888640 1.4529327 6.077078 145-146
GTGGC 5067065 1.4435092 10.628075 8
CATCG 5712540 1.4426311 6.247117 1
AGCTA 9235075 1.4421201 12.693532 1
ATTGG 9895590 1.4341747 17.98912 4
AACCT 7662715 1.4327081 38.04963 5
TCGTT 7385905 1.4242672 12.386952 2
GGTTT 8835415 1.4229861 6.9935 5
GTGCT 6026145 1.4124237 11.43601 8
GTTCA 8133630 1.4114283 10.089153 1
CTGTT 7303135 1.4083061 13.663538 8
GGTCA 6652110 1.4030446 7.9036407 7
AGCAA 9933115 1.395832 5.549619 2
CATGG 6617395 1.3957225 23.728462 4
TTGTC 7233710 1.3949186 16.690165 8
GCGGT 4887165 1.392259 68.41363 1
GTGTT 8627810 1.3895504 19.69138 8
CGTTC 4905670 1.376695 19.913124 1
ATCGT 7914370 1.3733802 5.4979267 1
CAAGG 7197850 1.3661596 8.355131 2
TATCG 7865320 1.3648685 7.4338093 9
CGGTG 4788030 1.3640174 14.21142 6
TGGAA 10413110 1.3580867 14.375696 9
GCTTG 5785210 1.3559526 19.87182 6
CGGTA 6415905 1.3532249 50.78901 2
ATCTT 9438170 1.347487 9.083273 8
GCTAC 5318005 1.3429961 20.48567 2
GAACC 5902720 1.34142 46.487255 4
GTGAT 9251185 1.3407806 23.594894 1
TGAAC 8580050 1.3398336 5.446539 9
ACGTT 7716300 1.339009 6.160539 3
GGTAT 9236820 1.3386985 34.934204 3
GACTT 7640130 1.3257912 24.440487 3
AGGTT 9121110 1.3219286 16.217793 1
CGCCT 3212965 1.3121911 86.54808 1
TCCAT 6294990 1.3079269 23.361025 2
CTGAT 7525465 1.3058933 9.1010065 1
TTCAG 7521250 1.3051621 8.823287 8
AGGTA 9996350 1.3037322 8.6662 2
CCATG 5162370 1.3036925 28.404968 3
TAAAA 18214665 1.3021655 8.00828 9
GTAAA 13484055 1.3020165 6.7741504 8
CGTGC 3811745 1.3001723 9.409981 7
CGAAC 5705380 1.2965739 46.47806 3
GTATT 10868500 1.2959622 59.09675 4
GTTCT 6612725 1.2751704 19.246964 1
GGTTC 5437005 1.2743393 12.449126 3
CACCT 4200495 1.2701077 17.616362 4
TTGCA 7261935 1.2601632 7.1184406 8
CACAG 5528305 1.2563328 6.374231 2
ACGTG 5954090 1.25582 25.08875 6
TGCAA 8033485 1.2544837 8.388273 9
ACTGT 7168290 1.2439129 17.238466 1
ACATG 7835335 1.2235411 16.17722 2
CCTAG 4783995 1.2081386 8.705529 4
CTCAG 4744850 1.198253 6.2200875 8
CATTG 6899640 1.1972941 6.638172 6
GTCAG 5653330 1.1923846 7.987839 8
TAACG 7625050 1.1907039 13.916912 4
CTTGT 6155485 1.1869981 13.538166 5
CGTGT 5050705 1.1837975 15.92817 7
TCACA 6312420 1.1802417 7.885901 9
TTCAC 5671605 1.1784046 12.10304 2
ATTTG 9857990 1.1754687 36.59612 6
CAACG 5145800 1.1694068 15.575573 1
TCTTT 7369145 1.1691418 13.023848 9
TTGTT 8820300 1.168744 20.367525 8
GTTCG 4984315 1.1682369 19.981321 4
TTATG 9786530 1.1669478 18.7607 6
GATAA 11921985 1.1511835 8.593942 2
CCTAA 6123560 1.1449302 51.572933 3
CTGCC 2800255 1.143638 6.310526 8
GATCG 5420640 1.1433063 5.8961906 145-146
CAGGA 6022725 1.1431196 20.968674 2
CCATC 3774220 1.1412145 8.089519 6
GTATC 6562215 1.1387408 5.094461 8
GCTTA 6542210 1.1352693 8.050993 6
ACACA 6739860 1.1339988 5.86696 4
ATCCT 5457530 1.1339256 7.155621 2
CTGTG 4835615 1.133384 20.080307 2
CCACA 4159670 1.1318414 5.863807 2
GATAT 10548030 1.1318288 35.34633 4
GCGTC 3313850 1.1303422 6.644247 145-146
AATTC 8796150 1.1300977 11.67642 1
GTAGC 5351620 1.1287488 5.4211693 8
ACCTG 4460870 1.1265373 21.638811 6
ACGAA 8004055 1.1247544 14.514842 2
TCTAA 8734865 1.122224 6.43076 9
ATTAT 12705975 1.1217077 31.841642 5
TTAAA 14101145 1.1202443 6.2464185 8
CTACC 3704435 1.1201136 24.520683 3
GACAA 7961710 1.118804 8.447242 3
TGGCA 5247750 1.106841 8.623749 9
TCAGG 5219485 1.1008792 9.963139 1
TGTTC 5685065 1.0962843 14.616167 9
ATCGA 7012515 1.0950521 15.746276 1
GGCCT 3148135 1.0738174 93.595604 1
ATCCA 5739795 1.0731772 21.006128 1
TGGAT 7337535 1.063434 16.164236 5
ATCGC 4207580 1.0625721 5.5326595 1
TTTCT 6695700 1.0622975 17.499374 7
AGTCA 6756885 1.0551338 6.732255 7
TACTG 6066965 1.0528 5.989741 4
TCTTA 7371455 1.052422 8.729747 9
ATTCA 8176120 1.0504385 9.818448 7
TTACG 6038775 1.0479083 19.93924 4
CGTAT 6023830 1.0453149 44.192738 3
ACGTC 4108700 1.0376012 18.4853 6
ACACT 5547160 1.0371599 9.44845 3
TCGAC 4067255 1.0271348 17.716602 1
CTCTA 4892470 1.0165215 8.896068 3
TATTT 10352750 1.0156424 68.669334 5
TTCTG 5238580 1.010186 19.28852 2
GATTA 9402365 1.0088961 38.825436 4
TGTTA 8425185 1.0046209 9.263678 9
TACCA 5372290 1.0044643 15.163738 4
ATTCT 7028220 1.0034186 13.461879 7
TGGCG 3501820 0.99760103 6.5743794 9
TCGAA 6331605 0.9887234 15.789641 2
TCCAC 3269505 0.98860335 6.5072145 1
GACTC 3864560 0.97594666 12.813475 4
TGTCA 5619385 0.9751315 16.751797 9
TGTAG 6714370 0.97311825 6.615429 4
ACGTA 6229580 0.97279155 9.150893 6
ACTCT 4655615 0.9673096 20.819698 5
TTCCG 3439800 0.965323 25.51822 3
CGATT 5539110 0.9612014 62.785667 3
TATCT 6732430 0.9611886 9.396738 7
TGTCT 4930785 0.9508322 28.816336 5
GTTGT 5890435 0.948683 7.681157 5
GGTCT 4030325 0.9446381 11.775693 7
TTTGC 4894250 0.94378686 11.519672 7
GTAGT 6491615 0.9408342 10.529325 5
TTCTA 6584965 0.94013506 5.0316586 8
CACTC 3101515 0.93780804 15.290576 4
ATCTC 4500645 0.93511105 6.7326427 8
CATGA 5873550 0.917195 16.1788 3
GATGT 6312165 0.9148264 21.091875 3
TCACC 3011465 0.91057956 17.615269 3
TGTCG 3878550 0.90906477 11.888244 3
ACCTA 4848030 0.9064427 8.517492 6
ATGTC 5202110 0.9027218 16.184687 4
AGTCT 5192515 0.9010568 8.438395 7
CATTC 4320580 0.89769846 5.4891014 6
CCGGT 2630305 0.8971875 30.814377 5
CGACT 3530585 0.89160544 35.55593 2
CTGAC 3528780 0.8911496 5.890619 1
CTCTG 3171740 0.8900963 12.279131 6
CATCT 4279425 0.8891476 9.687481 5
CGGTC 2605735 0.88880676 10.527181 6
CTATT 6209170 0.88648283 30.24488 4
ACAGG 4660050 0.88448244 11.998336 1
CCTCT 2631930 0.88435733 8.5197935 7
GATCT 5092390 0.88368213 7.6565623 5
CTAGC 3495590 0.88276786 5.854823 2
AGACT 5650495 0.8823634 7.921641 3
TGTGA 6075965 0.88059384 12.809304 9
ACCAT 4681210 0.8752521 17.39344 5
AGGAC 4611025 0.87517744 23.033503 1
TGTCC 3116450 0.87458014 14.01497 9
CTGTA 5038535 0.8743366 7.917716 3
TGTTT 6589595 0.873162 18.92581 9
TTATT 8899140 0.87303793 5.9456744 8
TTTGT 6585480 0.8726167 20.265661 7
GGACA 4593535 0.87185794 23.072899 2
GTACT 4929975 0.85549825 7.0339828 5
CTAAT 6634715 0.8524043 35.445324 4
TATTC 5970045 0.85234296 14.016329 6
CTAGG 4024485 0.84883314 11.269124 1
ATATT 9606035 0.84803903 28.802902 5
TCAGA 5429920 0.84791917 5.79764 9
TCCTA 4078320 0.8473634 7.1578746 3
TCGCA 3347690 0.8454175 8.668636 9
TCTGA 4852030 0.84197235 7.0338564 7
TCTGC 2995875 0.84074265 28.096502 3
CCATT 4043605 0.8401506 18.7586 5
GTCAC 3326300 0.8400158 6.4195223 8
GTGGA 4753870 0.8374238 8.496549 8
ACCTC 2756805 0.83357775 17.251837 6
GGCGT 2921250 0.83220774 72.27037 1
ATGTT 6868755 0.8190318 8.887787 8
AGCGC 2650655 0.8136111 10.07481 4
CCTAT 3912545 0.8129199 44.05736 3
ATTAA 10219070 0.8118387 5.6710305 7
CCTGC 1986890 0.8114559 6.0255103 7
GTTCC 2880140 0.80826366 19.90643 2
AACGA 5727500 0.8048459 6.224305 2
TGAGT 5497465 0.7967514 31.638597 5
ATTCC 3831715 0.79612565 18.878958 2
GAATT 7409070 0.7950108 13.309608 2
ACTCG 3141010 0.7932231 6.0493264 3
GGATA 6072055 0.79192257 11.585179 1
GTGTG 4029695 0.7888303 12.496279 6
ATGGA 6023515 0.78559196 6.732854 4
TGTGG 4008890 0.7847576 14.431644 9
CTACT 3767690 0.782823 5.00046 1
GACAT 5000465 0.7808568 16.142437 1
GGATG 4432590 0.7808283 10.67728 6
CGATA 4996345 0.7802134 51.446064 3
GCAAG 4094570 0.77715373 7.4979305 3
AGTAA 8000515 0.77252746 5.104774 7
ACGCC 2077005 0.7633351 8.041032 2
ATTTC 5306670 0.757633 29.452543 6
GTCGC 2219455 0.75704813 5.0423217 8
CACAC 2769725 0.75363904 9.48729 3
GTCAT 4326025 0.7506949 7.3212404 3
TTAGC 4325785 0.7506532 5.3947697 8
AGTGG 4260765 0.7505603 6.6039824 7
TCTGG 3195080 0.74887115 8.884146 7
GGAAT 5740455 0.748675 16.15087 1
GACAC 3277720 0.744877 11.483312 2
GTGTC 3174085 0.74395037 15.6995125 8
GATCC 2923785 0.7383656 8.691283 1
TCTCT 3197345 0.73822933 15.098104 7
TATGC 4236320 0.73512834 5.588283 7
GTGCA 3485035 0.73505396 6.7314267 8
CCTGG 2153540 0.7345646 5.552695 7
CGCCA 1993035 0.7324747 8.031464 3
GGATT 4975585 0.72111493 6.1323495 5
CCTCA 2367230 0.7157816 5.833926 7
ATAAC 6186340 0.7152268 10.30559 3
ATTGT 5932350 0.70737463 13.447879 7
CTAGT 4072725 0.7067397 13.29542 5
CTGGA 3346320 0.7057965 6.072789 8
TAGAA 7309365 0.7057902 6.273952 9
TCTCC 2077500 0.6980628 5.7712297 9
GGACT 3277990 0.69138455 9.992883 4
GCGCT 2016630 0.68786514 11.030401 5
CCTGT 2392585 0.67143935 12.807209 2
ATTAC 5211370 0.66953814 14.761307 3
GAGGA 4218030 0.6686429 5.00472 4
TAATT 7547630 0.66631913 24.149282 5
GCCAT 2626905 0.66339225 5.5238934 4
ACGAC 2916140 0.66270626 16.07437 1
TACGT 3798730 0.6591934 31.166128 5
GGATC 3073630 0.6482815 8.827533 6
AGTGA 4901180 0.63921607 6.060078 3
GTGTA 4393445 0.63674504 6.5585165 8
TAGCA 4069250 0.63544124 6.212661 9
ATTCG 3638765 0.6314347 6.029648 7
GTCTC 2245095 0.6300488 14.733212 6
ACATC 3366770 0.62948954 5.2232375 6
ATGAG 4824235 0.6291808 22.651415 4
ACGAT 4011080 0.62635756 6.9259315 3
TTCGA 3595135 0.6238636 5.017909 8
TCGAT 3532875 0.6130595 5.633962 5
ATCTG 3478225 0.6035762 6.504395 6
AGTGT 4163905 0.6034776 10.072344 7
CGTCT 2140570 0.6007155 12.635918 7
TATTA 6785845 0.59906733 6.1923985 6
CTGTC 2085325 0.5852119 11.092477 8
GAGTG 3320945 0.58500504 18.343855 6
TTGTG 3611435 0.581639 12.038757 8
TACGA 3716650 0.5803803 16.105171 1
GTCGA 2723920 0.57452166 6.9623613 4
AGTGC 2714690 0.57257485 6.624043 7
TCTGT 2940665 0.5670656 17.707918 7
TTGTA 4737555 0.564907 6.7642965 8
CGATC 2222335 0.56122315 11.204668 4
ATTTA 6321050 0.5580343 12.268984 6
CACAT 2978520 0.5568979 6.4918294 5
GCGTA 2566215 0.54125893 53.647472 2
TGTGC 2298995 0.53884447 6.948765 7
TAGGT 3694920 0.5355073 7.764904 1
TCCGG 1569035 0.5351921 31.022568 4
TGTGT 3254780 0.52419806 20.697706 5
CATGT 3010665 0.5224405 13.460947 5
GTCTG 2208195 0.51756257 18.648352 6
AGACA 3672040 0.5160063 7.0969543 1
AGCGG 1990195 0.5102056 6.25564 4
TAGTG 3507965 0.50841177 9.267282 6
TGTAA 4711060 0.50550795 8.750465 9
TCTCG 1796770 0.5042338 8.47766 9
TAGTC 2899855 0.5032117 7.077655 6
GCATG 2381615 0.50232357 10.733276 2
CAGAG 2553390 0.48463613 5.3297415 4
AGAGG 2993320 0.47450164 5.0065546 3
GAGTA 3590710 0.46830335 8.7326 6
TATGT 3922705 0.46774414 10.386777 7
TAGCG 2176290 0.459017 6.923922 3
TTCGC 1621325 0.45499802 12.626177 1
CTCTC 1329425 0.44670138 10.01764 8
GTGAG 2417425 0.42584446 8.18851 4
TCGCC 1031060 0.42109004 18.455763 2
GAGAC 2187360 0.41516325 9.631392 2
TGAGA 3127435 0.40788272 8.672615 1
ATGTG 2789200 0.40424067 7.581902 4
GTCTA 2212760 0.3839801 7.2273703 6
TCGCG 1111985 0.379294 7.421765 2
TTATA 4205470 0.3712669 6.105698 6
TCTAG 2139290 0.3712308 7.4323025 4
TTTCG 1919355 0.37012044 6.3480644 7
CGCGT 1064660 0.36315167 7.4289193 3
GTACG 1661915 0.35052648 13.880944 4
AGCAT 2154070 0.33637276 10.796295 1
GAGTC 1587410 0.33481207 13.99153 6
ATAGC 2107215 0.32905605 5.0899034 2