FastQCFastQC Report
Tue 9 Jul 2019
S_1_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename S_1_R1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 46874872
Filtered Sequences 0
Sequence length 100-150
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 8288575 4.0209503 10.193077 145-146
CACCA 18855070 3.7322962 5.9152317 105-109
ACCAA 21558505 3.3053086 4.71109 90-94
CAAGA 18295555 3.029823 4.5698504 115-119
CCAAG 14052405 3.0045328 13.1657 1
TCAAG 18973400 2.7781754 8.123078 9
GAAGA 15312930 2.7391028 6.8785644 135-139
GCTTC 16164090 2.7018628 11.59606 6
AAGAG 14691795 2.6279974 6.705265 4
AGAGC 11358480 2.6231606 8.648877 140-144
CTGCT 15525575 2.5951335 21.45121 4
GGAAG 10359825 2.5842555 8.202114 135-139
TCACC 14344555 2.5106022 11.164501 3
TTCAA 26712075 2.4798884 6.3469133 8
TTCTT 34146220 2.4782941 13.289226 8
GAGCA 10633695 2.4557765 8.152746 140-144
TCTTC 22024385 2.3341336 12.494322 9
AGCAA 13975830 2.314458 7.1362715 2
GTCAA 15657945 2.2927113 9.789963 8
TACCA 16791115 2.276228 12.762994 4
GCTTG 12335140 2.227073 23.856937 6
CTTCT 20794915 2.203835 8.661692 5
CAAGG 9512495 2.1968434 14.240791 2
ACCTT 17977405 2.1547956 7.6048617 5
AGCAC 10007350 2.139663 8.629793 145-146
TGCTT 18143555 2.0769339 29.232979 5
TCAGC 10824535 2.0463428 5.598528 9
AAGGT 12860810 2.034048 10.531122 1
TCAAC 14953320 2.027094 5.0669074 9
ACGAC 9351910 1.999524 21.437443 1
GAAGG 7897895 1.9701276 13.383504 3
TAGCA 13436530 1.967441 6.324507 1
TGAAG 12334015 1.9507308 5.1083326 9
TCACG 10256615 1.9389795 7.934998 9
ACCAT 14262550 1.933452 15.434644 5
GCAAG 8357575 1.9301227 9.951026 3
CGATA 13164105 1.9275513 48.866444 3
TGTTG 15383255 1.9020715 27.267717 9
AGCAT 12955340 1.8969828 10.627859 1
TCTTG 16537125 1.8930421 15.630831 9
CCATC 10752810 1.8819705 6.0852957 6
TTGAA 18523445 1.8574818 5.1636653 8
AGAAG 10358065 1.8528007 12.426029 2
TCAAA 17588445 1.8467541 6.9501367 9
GTTGT 14872820 1.8389584 10.345021 7
CCGAT 9652255 1.8247272 140.61739 2
TTCTG 15935000 1.8241156 14.671073 2
CATCT 15128380 1.8133079 7.3350687 5
TCTGC 10808395 1.8066467 21.441174 3
CACGT 9452315 1.7869292 6.081993 145-146
TTGTT 22661510 1.7765498 22.932137 8
GATAA 15618935 1.7713785 10.37143 2
AGTCA 12049410 1.7643323 6.3695307 7
ACGTC 9305555 1.759185 21.824839 1
CAACT 12863210 1.7437556 11.893796 1
TCTTT 23727035 1.7220812 17.398172 9
ACCTG 8988570 1.6992598 19.446507 6
TGGCA 8310455 1.6969627 20.262623 9
CACCT 9640240 1.687247 11.166527 4
ATCTT 20421575 1.6763185 8.162262 8
TTTCT 23069895 1.6743867 10.510475 7
GTCAC 8820065 1.6674047 7.8437643 8
GCCGA 5572020 1.6613911 221.89676 1
GAAGT 10494840 1.6598496 5.060499 3
AACTG 11297875 1.6542889 12.842673 2
CGGAA 7099820 1.6396531 7.7209225 135-139
GTGTT 13246740 1.6379008 29.222954 8
TTCAC 13637060 1.6345563 7.640852 2
TTGTG 13163770 1.6276419 10.546396 8
TGTTT 20730865 1.6251968 25.779745 9
ACGTT 12540655 1.6235949 8.765543 3
CCATG 8489690 1.6049482 23.520624 3
CTTGG 8880350 1.6033208 14.380261 3
CTTGT 13955250 1.597489 12.438733 7
GGTCA 7810465 1.5948668 7.222001 7
ACACG 7408435 1.5839913 7.215859 145-146
CTGAA 10736750 1.5721263 5.003949 145-146
AGGTT 11146540 1.5587478 18.119446 1
ACGAA 9410340 1.5583931 20.396109 2
CCTTG 9162635 1.5315542 5.566432 6
CGTTC 9158435 1.5308523 12.456296 1
GAACC 7152445 1.5292583 45.146584 4
ACTTC 12647595 1.5159577 9.851753 4
GATGA 9561075 1.5121665 11.005439 3
ATAGC 10282805 1.5056572 5.4450836 2
TTGGA 10760905 1.50482 11.128314 4
ATTTC 18207235 1.494553 18.80635 6
CGAAG 6462710 1.4925171 12.354651 2
TGGAA 9379275 1.4834133 8.541962 9
CTTCG 8869535 1.482562 5.72196 1
TGTCA 11400755 1.4760159 30.010258 9
TTCCA 12215625 1.4641812 8.939867 3
TGTGT 11787980 1.4575316 22.911703 5
TGAGA 9192185 1.4538233 29.255764 1
CAACG 6763300 1.4460555 20.180384 1
AGGAC 6241605 1.4414544 32.588383 3
CGTCA 7563560 1.4298663 28.192743 2
TGCTG 7888450 1.4242362 12.244726 9
ATCCA 10489490 1.4219705 16.844965 1
GTTCA 10977620 1.4212341 8.254282 1
GTCTG 7851815 1.4176219 18.576225 6
TTGGT 11442220 1.4147799 22.183048 5
GACAA 8467610 1.4022729 10.003169 3
ATCGG 6845315 1.3977867 9.164963 145-146
CGATT 10767495 1.3940301 53.163628 3
ATTTG 15679650 1.390215 35.251682 6
ATAAC 13134165 1.3790629 9.607895 3
TGCAG 6748680 1.3780544 8.192955 9
AGATC 9398555 1.3761814 6.5579653 145-146
AACGA 8214020 1.3602773 8.215596 2
CTGTG 7516965 1.3571657 17.785261 2
GGTTT 10944520 1.3532416 7.5773854 5
CACAC 6812525 1.3485159 8.422988 3
AACCT 9909375 1.3433294 28.500126 5
GAGAA 7493325 1.3403697 12.40183 1
GATCG 6536105 1.3346473 8.559004 145-146
GTTGC 7390355 1.3343066 23.2226 3
GTTGG 6788720 1.3239042 8.82046 4
GGATA 8245505 1.3040979 14.44605 1
TCTGG 7193475 1.2987605 10.622042 9
TTTGT 16532725 1.2960835 19.8914 7
TACGA 8848145 1.2955877 18.024073 1
TTGCT 11252305 1.2880769 14.723911 4
CAGGA 5576465 1.2878451 32.578022 2
TTGTC 11240520 1.2867278 20.97682 8
TGTGA 9182975 1.2841601 13.781113 3
GGTTG 6584355 1.2840499 25.075235 2
CGAAC 5974965 1.2775022 45.1571 3
TGAGT 9126335 1.2762394 31.970465 5
TTTGG 10304340 1.2740861 7.6068172 7
TATCA 13639480 1.2662582 6.887635 9
TGATG 9047395 1.2652003 35.55218 2
ATCTG 9730850 1.2598191 6.742977 6
TGAAA 11070035 1.2554775 6.3105454 9
CCATT 10416235 1.2485038 12.179902 5
TTACG 9643315 1.2484864 17.024883 4
GCCAT 6577275 1.2434123 5.2982955 4
TGACG 6089035 1.2433573 5.786851 9
TGGAT 8791305 1.2293884 19.082256 5
AATTG 12165620 1.2199361 13.59461 3
GTTCT 10648585 1.2189677 14.665856 1
CTGTT 10624005 1.2161539 16.111864 8
TTTGC 10586075 1.211812 9.863094 7
GTGAT 8585070 1.2005482 24.414669 1
TGCAA 8191320 1.1994122 5.1738744 9
GACTT 9259980 1.1988573 23.597002 3
GGTGT 6144235 1.1982198 25.082851 7
TCGAT 9250095 1.1975775 5.913137 5
GTATT 13416730 1.1895763 48.73451 4
CGTTG 6582530 1.1884562 14.245583 4
GCACA 5558270 1.1884092 7.263309 145-146
GTCTT 10358780 1.185793 21.538115 8
TGGTT 9566135 1.1828103 10.79805 5
TTCGT 10287370 1.1776186 13.75836 5
GAGTG 5334215 1.1765119 27.135597 6
GCTAC 6217025 1.1753083 17.792978 2
TGGAG 5327820 1.1751014 7.4270077 1
CGCCT 4808220 1.1735675 58.39913 1
AGGAT 7383775 1.1678078 6.3425884 5
TCCAT 9657420 1.1575512 14.90368 2
CGTCT 6906495 1.1544356 11.510434 7
AGTGT 8223020 1.1499186 16.97319 5
GTGTG 5891545 1.1489414 17.610163 6
TGGTG 5889340 1.1485113 27.872644 6
TACGT 8857910 1.1468027 25.494326 5
ACTGT 8854255 1.1463295 15.0858755 1
GTCAT 8848745 1.1456162 16.180336 3
TGCCA 6053105 1.1443197 17.566748 9
CAGTA 7764060 1.1368508 5.317955 1
CATGT 8780985 1.1368436 20.523907 5
TCTGT 9928035 1.1364846 18.45775 7
TTATC 13827230 1.1350175 10.1023855 6
ACGAT 7728465 1.1316388 7.261136 3
ACGTG 5540795 1.1314089 29.421795 6
CGCCA 4094215 1.1301903 7.7387586 3
ACTTG 8647905 1.1196141 12.959987 4
ACTGG 5482385 1.1194818 17.912067 3
GAGAT 7062620 1.1170144 8.144539 5
GTAGC 5469280 1.1168057 5.1638274 8
AACGT 7626620 1.1167262 13.35363 5
TGTTC 9743740 1.1153879 17.100945 9
AGTGA 7049210 1.1148933 7.338027 3
TCGCC 4566980 1.1146868 18.127556 2
AGCTA 7596535 1.112321 13.819988 1
TTGCA 8582025 1.1110848 7.033828 8
TACTG 8551735 1.1071632 7.6431646 4
GACAT 7444000 1.0899861 16.8656 1
TGTGG 5584735 1.0891087 9.511702 9
GCGGT 3821045 1.0880845 76.23028 1
TCAGG 5317245 1.0857608 11.235081 1
CATGG 5291815 1.0805682 25.407537 4
GTGAC 5283835 1.0789386 20.120789 4
CGGTA 5264885 1.0750692 54.67214 2
AGAGG 4288035 1.0696491 10.107026 1
AGACA 6408640 1.0612985 9.35699 1
CATCG 5608900 1.0603443 6.1630187 1
TTGGC 5862430 1.0584443 12.325845 8
TCGGA 5171480 1.0559962 6.3780365 135-139
GTGAA 6676360 1.055924 6.6168804 8
GGACA 4551385 1.0511101 29.539446 2
GTCAG 5135045 1.0485563 5.456797 8
GTCGA 5124305 1.0463632 9.374582 4
GGTTC 5794295 1.0461427 13.5362625 3
GAATT 10424345 1.0453256 13.592288 2
ACATC 7683740 1.0416191 5.7595606 6
ATTGG 7439530 1.0403543 18.955072 4
GGCGT 3638940 1.0362281 80.34554 1
TGACT 7951455 1.0294471 12.694613 5
GATGT 7340415 1.0264938 25.843706 3
ATTGT 11478725 1.0177457 15.444797 7
GTGGA 4606105 1.0159203 8.401719 8
GTGTC 5617845 1.0142852 25.190798 8
AATTC 10888720 1.010884 9.237184 1
CATGA 6883100 1.0078564 16.86814 3
TGGTC 5575270 1.0065985 15.936979 6
GGATG 4549935 1.0035315 17.409204 6
GGAAT 6333370 1.0016772 21.43346 1
CTGAC 5282880 0.99871117 6.500453 1
AGGAG 3973365 0.99115473 10.094931 3
GTTCC 5928520 0.990965 12.457988 2
CATTG 7653385 0.99085706 6.50402 6
GATTA 9860630 0.98879766 41.178802 4
GACTG 4825905 0.98543113 8.366105 6
GCCTA 5204265 0.9838493 93.95543 2
ACAAC 6370005 0.976637 9.259921 4
AGAGT 6169440 0.9757502 19.252851 3
GAGTC 4777110 0.9754674 20.219183 6
ACTGA 6635055 0.97153646 5.031064 4
TATTT 17265575 0.9705918 44.092545 5
ATGGT 6889205 0.963396 17.215107 5
GTTCG 5300825 0.9570482 21.804482 4
GAGGA 3810490 0.95052564 10.118213 2
CTGGC 3584070 0.9448842 8.152844 8
TGTCC 5640555 0.94283104 17.486126 9
GGCCT 3575810 0.94270664 67.925644 1
TATCT 11395615 0.93541664 7.576609 7
GATAT 9239105 0.9264729 33.445023 4
ATGAG 5855540 0.9261041 29.216278 4
CTGCC 3787060 0.9243277 6.743066 8
CAGAG 4001560 0.92413193 8.698501 4
GTGGC 3240715 0.9228291 14.842682 8
CTGAT 7057035 0.9136496 10.306729 1
AGTGG 4127740 0.9104124 8.177031 7
TCATG 7013435 0.9080049 11.822247 4
GCGTA 4386410 0.895688 57.618973 2
TGTTA 10087240 0.89437175 9.917623 9
CAGGT 4376375 0.89363885 5.424295 4
CTGTC 5333945 0.8915805 14.040828 8
CTGTA 6874435 0.89000905 7.1200066 3
TAGGT 6357630 0.8890598 10.613924 1
ATTCA 9569835 0.8884417 5.8336654 7
GTGCA 4312785 0.880654 8.409983 8
CCTGT 5267450 0.88046575 9.334147 7
ATGTC 6796690 0.87994355 13.632643 4
GTACG 4276920 0.8733305 13.59479 4
AGTAC 5954245 0.8718489 5.3248396 2
TGTGC 4817315 0.86975193 12.440411 9
CTGGT 4816825 0.8696634 15.838484 4
CTACC 4956345 0.8674658 16.480001 3
GGTAT 6185075 0.86492956 37.442097 3
AGGTA 5459515 0.8634695 10.698762 2
ATGTT 9674970 0.8578182 12.502228 8
GGATT 6131905 0.8574942 5.856411 5
TGTCT 7487100 0.85706526 18.814968 5
TCGTC 5075700 0.8484143 7.133311 6
TAGAG 5362155 0.84807104 6.396943 2
GGAGT 3823935 0.84340537 7.4263687 2
TCGTT 7343450 0.84062135 10.522948 2
TTGTA 9475230 0.8401087 5.248255 8
CTCTT 7839705 0.8308481 9.631905 8
AAGCG 3585810 0.82811743 6.8227205 3
AGACT 5653150 0.82776123 9.280385 3
GTGTA 5918890 0.82770586 6.505268 8
TGGCT 4583635 0.8275617 6.529276 9
TCTTA 10080940 0.82750064 5.534041 9
GTGCT 4563730 0.8239679 10.23864 8
GACAC 3839475 0.8209149 12.0525055 2
CTAGG 4017140 0.82028437 15.554868 1
AGTCT 6318245 0.8180011 10.956154 7
CCACA 4127205 0.81696606 5.4923005 2
ATTTA 12784200 0.8128048 8.037207 6
CGGTC 3077560 0.81135076 9.404518 6
AGTGC 3965855 0.8098122 8.879649 7
TCGAA 5521905 0.80854374 12.894585 2
TGGTA 5766620 0.8064122 7.5512514 6
ATGGC 3896310 0.7956114 5.6852036 8
CGTGT 4404375 0.79519683 19.375679 7
TCCGG 3008755 0.7932113 26.218351 4
ATTCT 9658290 0.79280716 9.254163 7
GTATC 6121295 0.7925025 5.247222 8
GGATC 3879965 0.7922738 11.621125 6
GGTAC 3869385 0.79011345 13.802219 3
CACTG 4177950 0.78982776 6.5628233 2
CGACT 4174235 0.78912556 34.467648 2
TTCGC 4719195 0.7888238 12.413245 1
ATCGA 5377940 0.7874637 12.903169 1
TGGGA 3554535 0.78398657 6.5845857 9
TGTAG 5600895 0.783237 7.690372 4
TGCAC 4110280 0.77703506 7.5816975 9
TGTCG 4287505 0.7740963 15.409497 9
TTCCG 4629890 0.77389616 16.626614 3
GCATG 3763700 0.768533 11.793686 2
GGAGA 3072435 0.76641804 10.073092 4
ACATG 5234015 0.7663895 16.87884 2
GTGAG 3470965 0.76555437 10.228271 4
AGTAG 4809680 0.76069236 5.3079 4
ATTCC 6341965 0.76015633 11.925632 2
CTAGC 3997765 0.75576437 6.3032155 2
GGTGA 3404360 0.750864 5.71998 7
AGCTC 3970715 0.75065076 8.279547 1
ACAGG 3232075 0.7464248 19.870975 1
CTTGC 4428845 0.74029106 6.707365 7
TAATT 11475335 0.7295887 16.276863 5
TTGCC 4358700 0.72856617 10.681764 8
ATGGA 4569355 0.7226829 9.13666 4
TGTAA 7191670 0.72116154 6.2307034 9
TCTCC 4582385 0.70912904 10.476767 9
GTCGC 2673675 0.7048727 5.2199416 8
ATATT 11060515 0.70321494 20.997921 5
ACAGA 4228865 0.70031834 6.2409873 3
TCGAC 3696505 0.6988122 15.548974 1
GTAGT 4947325 0.69184077 12.269201 5
GAGAC 2976320 0.68736 14.633124 2
GCGCT 2601825 0.68593067 9.716196 5
ATTAC 7374890 0.684668 12.212217 3
GAGTA 4286010 0.6778695 8.643804 6
TAGCG 3301140 0.67407995 7.6169124 3
GGTGC 2364740 0.6733856 11.620769 7
ATTGC 5177535 0.67031723 8.665124 7
CACAG 3131290 0.6694985 8.058414 2
ATCGT 5148990 0.66662174 5.9130445 1
CTCTG 3970740 0.66371775 10.074169 6
TGCCG 2516470 0.66342807 6.3344693 9
ACCTC 3768625 0.6595895 10.586426 6
GATCC 3487575 0.65931463 7.1902328 1
CGTAT 5080065 0.65769833 36.52102 3
GAGAG 2622565 0.65419805 30.35482 2
TATTG 7296810 0.6469618 13.901153 6
CCTAA 4772190 0.64692503 34.954296 3
TCTCA 5387630 0.6457685 12.390641 9
CGCGT 2428185 0.6401531 7.8302946 3
TTCTC 5942395 0.62977225 9.759116 8
ATGTG 4477165 0.626093 11.127808 4
TGGCG 2155420 0.6137794 9.204645 9
TAACG 4145920 0.6070653 13.3959 4
TATTC 7394305 0.60696644 8.410814 6
ATCTC 5048635 0.6051361 6.0849643 8
ATTAT 9507325 0.6044649 26.01803 5
GGTCT 3305180 0.5967404 13.387865 7
CGTGG 2088430 0.5947033 8.016049 7
ACGCC 2147780 0.5928854 7.746348 2
GCTCT 3533640 0.5906556 7.3181047 2
GTACT 4541595 0.58798444 7.906383 5
GTCGT 3256150 0.5878882 5.3492947 4
GTCTC 3512365 0.58709943 16.476044 8
TTGCG 3240990 0.58515114 6.205442 8
ATCGC 3044650 0.57558113 5.616583 1
ACTCT 4755320 0.569979 14.237566 5
TAGTC 4320485 0.559358 6.3654785 6
CCTAG 2916625 0.5513784 7.184487 4
GTGCC 2088100 0.550495 11.997129 8
GCTTA 4179865 0.5411525 5.437845 6
GTGCG 1887895 0.5375988 7.434424 8
CTAGA 3608075 0.5283116 5.9188566 1
AGCGC 1767980 0.52715296 11.121986 4
GTGGG 1683450 0.51779634 5.7077436 8
CGATC 2738925 0.5177849 9.372551 4
CCGGT 1928275 0.5083597 25.972483 5
GGACT 2436275 0.49747792 11.248376 4
GACTC 2617320 0.49479583 11.977109 4
CCTAT 4065200 0.48726028 28.688726 3
TGCCT 2905910 0.4857292 5.3867188 9
ACGTA 3287385 0.48135462 7.099575 6
GGTGG 1562885 0.4807129 9.626699 7
TAGTG 3423630 0.47876516 11.400079 6
ACCTA 3528475 0.47832522 5.0426803 6
TCTCT 4499765 0.47688287 9.921673 7
CGTGC 1794760 0.4731605 9.780285 7
CTAAT 5092340 0.47276124 23.935165 4
GATCT 3640300 0.4712969 7.55914 7
AGCGG 1450435 0.46712798 9.495026 4
TGCGG 1630045 0.46417317 8.0356245 9
CGGTG 1623145 0.46220836 18.299475 6
CACAT 3340330 0.45282 5.738963 5
CACTC 2581030 0.45173514 9.863738 4
ACACT 3244600 0.43984273 7.6421432 3
TCTAG 3299440 0.42716697 5.6676226 4
ACACA 2685260 0.41169894 6.526294 2
AACGC 1916870 0.40984437 5.9969397 1
TCTCG 2288080 0.3824575 9.568891 9
TCGTG 2084325 0.3763187 9.209538 6
CTATT 4558865 0.37421748 19.6421 4
CTCTC 2369390 0.36666566 6.7374372 8
CTCTA 2876105 0.34473377 5.2506084 3
TTATG 3711620 0.32908583 18.092855 6
TCGCG 1141650 0.3009782 7.8274336 2
ACTCG 1561335 0.2951653 5.14182 3
TATGC 2200180 0.28484955 5.9440475 7
CTAGT 2192330 0.2838333 10.470747 5
TATGT 2820895 0.25011086 12.6769085 7