FastQCFastQC Report
Tue 9 Jul 2019
SL_1_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SL_1_R1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 44301365
Filtered Sequences 0
Sequence length 100-150
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 15173440 7.8565307 19.121447 145-146
CACCA 17051845 3.6233594 6.3084836 105-109
ACCAA 20568080 3.5301225 6.0898767 85-89
CAAGA 16021105 3.0168397 4.5536456 30-34
CTTCA 24561835 3.0166276 4.518187 70-74
AGAGC 11088075 2.8361142 7.3150187 140-144
CCAAG 12084930 2.817396 12.238963 1
TCAAG 17336905 2.761619 7.250025 9
GCTTC 15868285 2.6472723 8.325503 6
TCACC 14390330 2.5866826 9.9647255 3
GAAGA 12396860 2.5611513 7.377348 3
CTGCT 15166875 2.5302575 19.59533 4
TTCAA 24949215 2.474992 5.634624 8
AAGAG 11873790 2.4530866 7.3875175 4
TTCTT 34287760 2.4340014 8.525741 8
GCAGC 7670905 2.4291713 5.460376 95-99
GACCA 10178985 2.3730574 5.2548947 85-89
GTCAA 14774280 2.3534153 10.154278 8
GGAAG 8322255 2.3354595 7.190866 135-139
TCTTC 22454555 2.3329086 8.91195 9
TACCA 15966155 2.318082 11.798769 4
CTTCT 21808845 2.265823 6.9783745 5
AGCAA 11989645 2.2576993 8.717604 2
ACCTT 18236475 2.2397616 6.7349205 5
TAGCA 13627310 2.1707127 7.399585 1
GCTTG 11796705 2.1592011 23.29481 6
ACGAC 9249565 2.156379 22.469099 1
TCAAC 14803670 2.149304 5.1366568 9
GGCAA 8164950 2.0884354 6.373092 40-44
AGCAT 13010995 2.072539 10.972649 1
GAAGG 7348035 2.0620658 13.735508 3
TCTTG 18053185 2.0578365 10.384356 9
GAGCA 8017585 2.0507421 6.784089 140-144
TGAAG 11648555 2.0357666 5.3860865 9
TGTTG 16235690 2.0304468 28.882818 9
ACTGC 10206165 2.0127914 5.483333 15-19
TTGAA 18227310 1.9838244 5.8944955 8
TGCTT 17327710 1.9751416 25.722755 5
CAAGG 7701830 1.9699782 13.460687 2
GTGTT 15652875 1.9575592 30.269318 8
GTTGT 15111635 1.8898714 9.644053 5
ACCAT 12868645 1.8683631 14.492117 5
AGAAG 9030280 1.8656267 12.824513 2
CGATA 11686035 1.8614844 51.829044 3
CCGAT 9331930 1.8403807 133.03499 2
CAACT 12643325 1.8356495 11.825781 5
TCAGC 9270835 1.8283318 5.427609 9
TGGCA 8432150 1.8244765 22.914455 9
GCCGA 5739790 1.8176386 213.76793 1
CTGCA 9171230 1.8086884 6.0827537 8
TCAAA 15410990 1.8072364 7.463379 9
AAGGT 10340575 1.8071767 10.744972 1
AGCAC 7664600 1.7868713 7.565114 145-146
GACGG 5119310 1.7786344 5.127191 55-59
GATAA 13701940 1.7629107 11.327842 2
CACCT 9782295 1.7583818 9.966027 4
AGTCA 11024475 1.7561038 6.3492064 7
CAACG 7494085 1.7471186 20.503134 1
TTCAC 14072910 1.7284023 6.8049808 2
AACTG 10847605 1.7279297 12.690804 2
CATCT 14058120 1.7265856 6.626363 5
TTGTG 13665360 1.7089994 9.48508 6
TTGTT 21684680 1.6888789 23.22153 8
GCAAG 6589130 1.6853713 11.837746 3
GTCAC 8545250 1.6852368 8.4042425 8
ACGTC 8525510 1.6813439 23.638039 1
TTCTG 14682575 1.6736293 13.370709 2
GATGA 9548270 1.6687093 12.403449 3
ATCTT 19847990 1.6655812 5.9632115 8
CTTGT 14596920 1.6638658 11.365229 7
TCACG 8386785 1.6539854 7.518247 9
TTTCT 22979485 1.6312557 6.2557116 7
CGTTC 9736960 1.6243964 11.183354 1
TGTGT 12946120 1.6190506 23.956772 5
CTGTG 8772250 1.6056223 19.731888 2
ACTTC 13037275 1.6012077 8.339277 4
CCATG 8086360 1.5947375 25.019285 3
GGTTT 12727105 1.5916605 6.498777 5
TGTTT 20422225 1.5905545 25.148502 9
CTTGG 8677880 1.5883493 14.949568 3
TCTGC 9504515 1.5856181 19.588568 3
TCTTT 22222090 1.57749 12.322981 9
AGGTT 10607170 1.5681512 16.574974 1
ACCTG 7899410 1.5578687 20.539776 6
TGCAG 7185190 1.55467 12.306178 9
ACGTT 11472860 1.5459533 8.834514 3
TGAAC 9631645 1.5342379 5.1385427 9
CGGAA 5989975 1.532119 6.61741 135-139
TACTG 11352150 1.5296874 9.008046 4
CGAAG 5976205 1.528597 12.457851 2
ACGAA 8060760 1.5178741 21.08708 2
AGGAC 5934075 1.5178212 38.050694 3
TGTCA 11214240 1.5111045 27.015062 9
TGTGA 10018680 1.4811496 15.938733 3
CGAAC 6336105 1.4771552 45.314556 3
TTCCA 11989385 1.4725084 8.239432 3
GAACC 6229395 1.4522775 45.30072 4
GACTT 10724745 1.4451457 22.110085 3
ATAGC 9048955 1.4414203 5.641882 2
GGTCA 6637725 1.4362142 7.468415 7
CGCCT 5879710 1.4356089 50.51012 1
CTTCG 8573300 1.4302653 5.640467 1
CGTCA 7220575 1.4239933 29.477385 2
AACGA 7516960 1.4154743 8.257813 2
GACAA 7494100 1.4111698 11.048178 3
ATTTC 16792620 1.4091841 13.199766 6
AACCT 9612465 1.3956072 27.966846 5
ATAAC 11900605 1.395576 10.329765 3
GTTCA 10299535 1.3878492 7.4648733 1
TGAGA 7935960 1.3869327 31.585394 1
TGAAA 10716595 1.378812 8.150589 9
ACTGT 10226475 1.3780044 16.813547 1
GTTGC 7480630 1.3692116 20.30972 3
TGGAA 7814840 1.3657652 7.3158064 9
TGACG 6309170 1.3651241 7.483607 9
AGCTA 8531735 1.3590317 12.9985695 1
ACGGT 6280940 1.3590161 5.3131995 55-59
CCTTG 8145980 1.3589765 5.231065 6
ATTTG 14699350 1.3533533 35.387566 6
TTGCT 11787025 1.343573 12.649352 4
TCGCC 5439240 1.328062 19.125584 2
GTGAT 8958305 1.3243848 24.007193 1
ACACG 5617800 1.3096946 6.1052804 145-146
AATTG 12003240 1.3064088 14.864036 6
TGACT 9659965 1.3016677 14.4114895 5
CATGT 9625950 1.2970845 21.08293 5
TTGGA 8761330 1.2952645 12.059734 4
CTGAC 6558670 1.2934569 6.922921 1
TCAGG 5976780 1.2932045 11.422769 1
TTGGT 10326135 1.2913935 22.455925 5
TCCGG 4788410 1.28273 26.19266 4
AGATC 8048150 1.2820008 6.010744 145-146
TGCTG 7003930 1.2819592 13.783299 9
AACGT 8012225 1.276278 13.915533 5
GTAGC 5860950 1.2681422 5.264902 8
TTTGC 11075300 1.2624453 10.797021 7
ACTGA 7870490 1.2537011 5.583534 4
ATCCA 8566515 1.2437487 18.396446 1
CGATT 9207570 1.2407084 47.11435 3
TACGA 7746465 1.2339449 17.8275 1
TTGTC 10786890 1.2295702 17.8341 8
GAGAA 5943300 1.2278666 12.775905 1
AGTGT 8272515 1.2229986 18.596188 5
GGATA 6997255 1.2228795 15.369065 1
AGGAT 6963780 1.2170293 6.5501447 5
TTTGG 9704005 1.2135895 7.0383496 7
TGAGT 8052720 1.1905042 33.652523 5
GCCTA 6026115 1.1884301 92.07607 2
TTACG 8800670 1.185879 16.376547 4
GCTAC 6012020 1.1856502 16.021858 2
ACTGG 5436240 1.1762471 17.247324 3
CGCCA 4054585 1.1702919 7.4589014 3
CACTG 5928605 1.1691998 8.07189 2
AGTGA 6684290 1.168184 7.5936337 3
GAGTG 4920930 1.168183 31.531534 6
GTGGC 3970230 1.1668724 16.234043 8
GTCTG 6361705 1.16441 18.062387 6
CTGTT 10202900 1.1630026 16.027075 8
TTTGT 14916150 1.1617221 18.300531 7
GTGAC 5362775 1.1603514 23.330322 4
TGGAG 4870360 1.1561781 9.254408 9
GGTGT 5747750 1.1542344 25.512259 7
TACGT 8524480 1.1486628 24.381727 5
GGTTG 5713210 1.1472982 22.275425 2
GTTGG 5704295 1.1455079 10.184293 4
ATCTG 8424675 1.135214 6.858336 6
GATCG 5242360 1.1342971 7.726977 145-146
CACAC 5334340 1.1334978 8.716419 3
GCCAT 5736080 1.1312313 5.091972 4
ACGAT 7084835 1.128553 6.9826174 3
ATCGG 5206215 1.1264763 8.208869 145-146
TTGCA 8352350 1.1254685 9.169288 8
TGCAA 7057920 1.1242657 7.6179857 9
CGTTG 6133030 1.1225545 14.233803 4
CAGGA 4385455 1.121714 38.039024 2
CGGTA 5133540 1.1107515 50.417797 2
TGTGG 5523450 1.1091915 8.375578 9
CTAGG 5115890 1.1069325 16.456638 1
TCTGG 6047515 1.1069024 8.2827 9
TCGAT 8206345 1.1057945 5.6910076 5
CCATT 8979465 1.102837 11.1969795 5
TAGAG 6300215 1.1010609 6.663501 2
TTCGT 9604025 1.0947384 13.741952 5
AGTGG 4595420 1.09091 8.845751 7
GGACA 4253130 1.0878679 30.572203 2
GTCTT 9529210 1.0862104 16.963388 8
CAGTA 6818440 1.0861186 7.331781 1
TATCA 10909510 1.0822364 7.432306 9
CGCTG 4014530 1.0754213 5.043136 6
GACTG 4964965 1.0742767 9.629951 6
GTTCG 5863670 1.0732524 22.270718 4
TTATC 12723960 1.0677549 6.8894386 6
GAGTC 4933330 1.0674318 19.063955 6
GTTCT 9350175 1.0658027 13.365261 1
GTGAA 6084610 1.0633805 8.021376 8
GGAGT 4471445 1.0614794 7.243262 2
TGCCA 5370390 1.0591123 20.153736 9
GTCCA 5361095 1.0572792 5.5576673 8
GGATG 4450760 1.056569 18.534426 6
GAATT 9705605 1.0563388 14.294159 2
ATTGA 9697710 1.0554795 5.4367332 7
GTCAT 7802680 1.0514011 16.954018 3
ACGTG 4841265 1.0475116 31.467403 6
TGTTA 11369095 1.0467404 10.717089 9
TCTGT 9174580 1.0457871 16.599821 7
GGCGT 3544040 1.0416127 73.12319 1
TGGAT 7043190 1.0412565 19.99528 5
TGGTG 5180615 1.0403452 29.86818 6
GTCGA 4795300 1.037566 9.2297535 4
TGATG 7008260 1.0360925 36.692795 2
ACTTG 7687255 1.0358477 12.961181 4
CATCG 5231185 1.0316591 5.549244 1
ACAAC 5985130 1.0272346 10.066462 4
AGGAG 3658680 1.0267287 10.365065 3
GAGAT 5858600 1.0238818 8.101968 5
GATTA 9393220 1.0223396 38.044537 4
TAGGT 6911100 1.0217285 11.123397 1
CATGG 4718970 1.0210503 27.446167 4
CTGGC 3810345 1.0207238 8.316538 8
GTATT 11058220 1.0181184 44.333317 4
AGAGT 5818330 1.016844 22.084946 3
TTCCG 6070605 1.0127461 16.316599 3
AGAGG 3606210 1.0120043 10.689899 3
GGTTC 5491810 1.0051894 13.595948 3
GTCAG 4643060 1.0046256 5.666196 8
TCCAG 5091445 1.0041007 5.036721 9
GAGGA 3546480 0.99524224 10.660849 4
GCGGT 3374790 0.9918693 68.47097 1
CAGGT 4563795 0.9874749 5.773491 4
TGGTT 7873590 0.98467654 9.877717 5
GTTCC 5870455 0.9793556 11.185048 2
TGTTC 8571070 0.97699445 17.207472 9
CGTCT 5855845 0.9769181 8.709543 7
GCGTA 4460815 0.965193 53.83506 2
CCTGC 3950420 0.96454704 5.733467 7
GCACA 4124060 0.9614545 6.228285 145-146
CTGAT 7132295 0.9610676 11.562861 1
CTGCG 3585595 0.9605172 5.8333673 8
GACAT 6012885 0.9578006 18.73316 1
TCCAT 7776395 0.95507884 15.572353 2
TTGGC 5203695 0.95245445 12.602063 8
GTCCG 3515125 0.9416396 5.128655 8
AGTAC 5885740 0.9375476 7.337203 2
TATCT 11162265 0.93670243 5.0661454 7
GTGGA 3937600 0.93474954 8.64864 8
GTGTG 4642835 0.9323509 19.12159 6
TGCGA 4294045 0.92910874 7.4601126 9
CTAGC 4708800 0.9286379 5.8246374 2
CATTG 6852365 0.9233473 6.733185 6
TATTT 16058610 0.9207583 39.027874 5
CTCTT 8826850 0.9170627 6.360145 8
CTACC 5091435 0.91519284 14.6100025 3
TGCCG 3413765 0.91448706 9.5366955 9
CAGAG 3572160 0.913689 10.39256 4
CCTAA 6292290 0.9135602 37.763206 3
TTCGC 5462325 0.9112681 13.068301 1
GTGTA 6107290 0.9028944 7.067494 8
ATTGG 6072210 0.89770824 19.419613 4
AGACA 4755600 0.895499 9.265314 1
TCGTT 7833685 0.89294183 11.286428 2
ATTTA 13169635 0.89264554 7.178535 6
CCACA 4193420 0.8910629 5.248464 2
ATGGT 6024915 0.890716 18.403654 5
GTGTC 4861420 0.8898064 23.07804 6
CCGGT 3288810 0.8810138 25.750906 5
TCGGA 4028650 0.87168485 5.106989 135-139
AATTC 8767100 0.8697067 9.70675 1
GGCCT 3239950 0.8679251 69.62885 1
ATTGT 9376555 0.86328936 16.484861 7
ATGAG 4918660 0.8596125 32.9434 4
GCGCT 3207135 0.85913455 9.293713 5
GGAAT 4908845 0.85789734 22.950552 1
ATGTT 9300570 0.85629344 12.927715 8
TGGTC 4650930 0.8512795 14.76261 6
CGACT 4309555 0.84990156 32.36779 2
CCTGT 5077090 0.84700006 9.513256 2
CTGCC 3462040 0.84530264 7.6133966 8
CTGTA 6236000 0.84029305 7.6771345 3
AAGCG 3279860 0.8389243 7.677166 3
GGATC 3867865 0.8368956 11.02931 6
GGTAT 5650550 0.8353705 34.449947 3
CGCGT 3103325 0.8313257 8.542265 3
ACATC 5725880 0.83132476 6.107592 6
TCATG 6135290 0.8267223 12.967522 4
TGGTA 5558870 0.8218166 7.3782864 6
CTGTC 4920505 0.82087743 11.889848 8
TCGTC 4917535 0.82038194 6.8842583 6
AGACT 5139890 0.81874007 8.683703 3
GATAT 7520275 0.81849194 35.38723 4
TGTCC 4874590 0.8132175 14.619566 9
GATGT 5441920 0.8045269 26.218163 3
ATGTG 5389360 0.7967563 12.673074 4
TGTAG 5347255 0.79053175 8.420142 4
TCGAA 4959940 0.7900756 13.125992 2
AGTAG 4507000 0.78766865 5.3080287 4
TGTGC 4287820 0.78481793 11.056717 9
TAATT 11552960 0.78306633 17.418352 5
GGTAC 3614405 0.7820541 13.520245 3
CATGA 4909240 0.7819995 18.73501 3
ATTGC 5798775 0.78137755 11.5494 7
AGGTA 4467870 0.78083 10.924751 2
AGTCT 5786460 0.7797181 9.107931 7
CACAG 3331125 0.7765952 9.481487 2
GACAC 3310905 0.7718812 11.42973 2
ATGTC 5718320 0.7705362 12.353472 4
CGGTC 2869145 0.768593 8.182152 6
ATTCA 7734810 0.7673024 5.3198695 7
CGTGT 4187475 0.7664513 18.80902 7
GCATG 3510970 0.75967366 11.950312 2
GATCC 3846520 0.7585849 7.210877 1
TAGCG 3504035 0.75817305 7.6692185 3
GAGTA 4307085 0.75273037 8.6202965 6
TTGCC 4506035 0.7517323 11.75495 8
GGATT 5038460 0.74487984 5.61778 5
TGGGA 3130720 0.74320376 6.4142604 9
TGTAA 6821900 0.7424821 7.2582483 9
ATTCC 6030210 0.74061644 12.015651 2
ATTAC 7463335 0.7403717 12.059618 3
AGTGC 3419260 0.7398303 11.391443 7
GTGCA 3416285 0.7391865 11.31156 8
CCTAG 3730585 0.7357209 7.203311 4
ACAGG 2859405 0.73138016 24.535192 1
GTATC 5362095 0.7225353 5.150213 8
GTACG 3335625 0.7217339 13.297354 4
ATGGA 4119710 0.7199836 9.646579 4
ATCGA 4519905 0.7199818 13.143863 1
CTGGT 3906085 0.7149473 14.589432 4
ACCTC 3955550 0.71101594 8.127253 6
TGTCG 3853510 0.70532435 14.040879 9
TGTCT 6178315 0.7042504 16.275448 7
TTGTA 7600360 0.699757 5.5378313 8
GTGCT 3822730 0.69969046 10.744282 8
TGCAC 3547530 0.69962007 9.484396 9
ATTCT 8335155 0.6994602 6.4309115 7
AGCGC 2196080 0.69544005 11.220952 4
ACATG 4356020 0.6938763 18.747002 2
TGGCT 3755700 0.6874217 5.800182 9
CTTGC 4078000 0.6803241 7.293298 7
GTGGG 2109485 0.68021756 6.2089744 8
TGGCG 2295095 0.674541 10.434195 9
CGATC 3414500 0.6733848 8.640947 4
GTGAG 2777835 0.6594322 10.302174 4
TTGCG 3582815 0.6557779 8.690932 8
GGTGC 2224150 0.65368986 14.297072 7
TAACG 4103295 0.65361947 14.025888 4
GATGC 2998800 0.64885473 5.765032 7
GTACT 4803690 0.647291 9.010193 3
TCGAC 3280965 0.6470499 13.403416 1
ATCGT 4794420 0.646042 6.890232 1
ATCGC 3266255 0.6441489 6.297471 1
GCTCT 3858740 0.64374536 6.8358297 2
GGAGA 2238260 0.6281188 10.336419 4
ATGCC 3155140 0.62223554 5.4812884 8
ATATT 9178745 0.62214065 21.671568 5
ACAGA 3292745 0.6200374 7.652985 3
ACTCT 4997730 0.6138096 12.511938 5
GAGAC 2374065 0.6072397 13.936738 2
GGTGA 2548050 0.6048834 6.8131223 7
GTCGC 2257715 0.6048018 5.6262584 8
GTAGT 4006490 0.59231454 12.718022 5
GTGCG 2012035 0.5913481 10.586106 8
GAGAG 2087775 0.58588856 35.463505 2
ATGGC 2685360 0.58103526 6.009065 8
TATTG 6300660 0.58009493 15.500318 6
CGTAT 4270645 0.57546395 33.514538 3
AGCTC 2909480 0.5737881 8.090733 1
GTGCC 2123770 0.5689203 13.978478 8
TGCGC 2095365 0.56131107 7.524755 9
ATTAT 8137780 0.55158347 23.52041 5
TATTC 6568345 0.55119497 6.8028727 6
CATGC 2715845 0.5356007 5.0579925 7
CTAAT 5345285 0.5302586 25.797062 4
ACGCC 1836705 0.53013587 7.468513 2
GATCT 3902050 0.52579623 6.2359934 7
CTCTG 3139640 0.52377945 9.477267 6
TCTCC 3432360 0.5219125 5.942169 9
CCTAT 4246500 0.52154523 25.418453 3
TGCGT 2765350 0.5061537 5.0165777 9
GGTCT 2765205 0.5061273 10.937203 7
ACGTA 3150155 0.5017925 6.712902 6
GGTGG 1548235 0.4992388 10.35654 7
CACAT 3187855 0.46283588 5.8983335 5
GGACT 2128470 0.46054015 11.440941 4
TCTCA 3739600 0.45928898 12.240408 9
TTCTC 4415675 0.45876512 5.9336286 8
GTCGG 1545840 0.45433086 6.119627 8
CTAGA 2838170 0.45209596 6.080759 1
AGCGG 1301130 0.45205986 10.403536 4
TAGTG 3054910 0.45163414 12.035867 6
TGCGG 1536660 0.4516328 9.921868 9
ACACT 3103180 0.45054215 8.061543 1
CGTGG 1532905 0.4505292 8.07915 7
GTCTC 2686195 0.44813222 13.383264 8
AACGC 1910355 0.44536677 6.027673 1
TAGTC 3083860 0.41554612 5.7408533 6
CGTGC 1528925 0.40957186 11.475528 7
CACTC 2261490 0.40650615 8.8085785 4
GACTC 2013280 0.39704558 10.743436 4
TGCCT 2348090 0.39172685 5.117783 9
CGGTG 1315135 0.3865254 19.690128 6
ACACA 2227465 0.3823023 7.087187 1
TCTAG 2802165 0.37758815 5.517279 4
TCTCT 3573400 0.37125722 5.952356 7
CTATT 4085870 0.34287342 17.358397 4
TCTCG 1925355 0.32120287 6.2307806 9
TCGTG 1673995 0.30639842 9.274331 6
TATGC 2057675 0.27726907 6.7050905 7
TTATG 2932440 0.26998657 17.865238 6
TCGCG 982690 0.2632452 8.54401 2
ACTCG 1327655 0.2618312 5.4912825 3
CTCTA 1973715 0.24240708 5.033564 3
TATGT 2421485 0.22294353 12.185842 7
CTAGT 1590745 0.21435086 10.237708 5
TGGGG 546335 0.17616938 5.094585 9
Produced by FastQC (version 0.10.1)