FastQCFastQC Report
Tue 9 Jul 2019
S_2_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename S_2_R2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 39536103
Filtered Sequences 0
Sequence length 100-150
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGGTG 17093055 3.561649 22.089058 6
TTGGT 19743505 3.1582458 23.641487 5
TCTTG 16716105 3.0672965 29.465967 9
TGCTG 12290540 2.9376595 10.663267 9
TGAAG 21509650 2.9346156 8.369514 9
CTTGG 11778925 2.8153744 14.230854 3
TGATG 19045775 2.8136394 31.985357 2
CTGGT 10819210 2.585985 18.976608 4
TCAAG 16239715 2.5415304 6.9626703 9
TTGAA 23437480 2.4548166 10.595113 8
TTGCT 13364385 2.4522781 21.56144 4
TGGCT 10233965 2.446101 9.67026 9
CCTTC 7432120 2.3374445 5.314242 6
GCCGA 6963575 2.2967815 231.15933 1
GGTGA 11835510 2.2775426 6.925801 7
TTGAC 13371405 2.2659268 5.179584 8
TCGTG 9369400 2.2394545 9.19099 6
CGTCG 6241470 2.229083 6.368731 145-146
GTCGT 9279575 2.2179847 6.4276137 4
AAGGT 16176975 2.2070653 8.425277 1
TGAAA 22488480 2.1752856 13.870061 9
TGGTA 14697205 2.1712234 9.85876 6
GGTGG 7924850 2.1509597 7.7871356 7
ACTTG 12647840 2.1433108 12.568638 4
CTCTT 10153265 2.137103 14.495535 8
CCTTG 7715795 2.1154866 7.325593 6
GCTCT 7599310 2.083549 10.956598 2
TTCAA 17014180 2.0441751 10.901149 8
ATGCT 11905600 2.0175304 5.3400874 15-19
TGGTT 12559205 2.0090177 12.138308 5
TCTTC 9436655 1.9862679 15.6116085 9
CTTCA 10185445 1.979921 6.26011 7
CTTCT 9377010 1.9737136 16.459684 5
CTGCT 7177850 1.9679947 25.711498 4
CAACT 10959645 1.9674898 14.193867 1
CTTGC 7163970 1.964189 5.4446216 7
CTGAA 12504015 1.9568899 7.2260118 8
CTCAA 10880590 1.9532975 7.8486786 8
GTGGT 9363105 1.9509733 5.560505 8
TGGTC 8136045 1.9446604 16.937244 6
TTGGC 8124270 1.9418464 9.835329 8
TTTGG 12098470 1.9353168 9.319227 7
GTTGC 7949140 1.8999869 28.090174 3
ATGGT 12853150 1.8988005 16.62329 5
ACAAC 11438015 1.8963337 8.973719 4
ACCTT 9739300 1.8931961 11.87984 5
GATGA 13868285 1.8920848 9.101537 3
TGCTA 11116425 1.8837962 8.850533 9
TGTTG 11770730 1.8828901 19.725792 9
GTCAA 11944310 1.8692955 9.868046 8
TGCAG 8429205 1.8606521 6.715712 9
GTCTT 10103580 1.8539412 19.088554 8
TGCTC 6565865 1.800203 5.5835037 9
TGCCA 7063920 1.788642 10.4436455 9
GTTGG 8560190 1.7836714 10.616072 2
TCAAA 16063065 1.7823117 10.35742 9
CCAAG 7607065 1.7788646 11.702193 1
TTGCC 6487405 1.7786913 8.469235 8
AACGT 11352795 1.7767229 12.785896 5
TTTGA 15579155 1.7668669 7.5643554 7
GGTGC 5655365 1.760763 7.897427 7
ACTGG 7971140 1.7595394 17.528069 3
GAAGG 9851920 1.7508482 9.175724 3
TTGGA 11712510 1.7302935 9.220709 8
TCGTC 6198865 1.6995803 7.6260066 1
GCCTA 6663470 1.6872447 122.03994 2
TTATC 12947200 1.6843598 15.660017 6
AGCTC 6637265 1.6806095 10.059962 1
TTCTT 11910190 1.6777592 21.782347 8
TGACT 9878410 1.6740016 14.440157 5
TTCCA 8609340 1.6735462 14.427412 3
CGTGA 7578525 1.672874 6.9770913 7
TCAAC 9142275 1.641233 9.926664 9
GGTAA 11947900 1.6300819 5.7637362 7
CTCGT 5920580 1.6232812 7.0152426 4
TCATC 8319130 1.617133 5.424489 4
CGAAG 7919285 1.6144055 10.503112 2
AATTG 15023520 1.5735472 12.364064 3
CAAGG 7695395 1.5687641 10.208097 2
GGTGT 7519580 1.5668414 16.041283 7
TGCTT 8522735 1.5638665 38.301304 5
CCGAT 6168400 1.561889 178.36235 2
GTGAA 11289815 1.5402976 10.634394 8
ACTTC 7905445 1.5367179 15.315716 4
TCTCA 7896660 1.5350103 23.152264 9
GGTAC 6902970 1.5237529 13.799979 3
GTGAC 6902695 1.5236923 18.960056 4
ATTGA 14517515 1.5205486 7.2633567 7
TATCG 8933725 1.5139145 6.9287887 9
CATGG 6812730 1.5038334 25.089502 4
GGTAT 10178065 1.5036092 37.641212 3
ATTGG 10169220 1.5023025 17.431131 4
AGCTA 9597705 1.5020497 12.754141 1
TTCGT 8181035 1.5011667 15.844033 5
CGTTG 6270800 1.4988337 12.6191435 4
AACTG 9573570 1.4982725 12.36616 2
TTCTC 7115860 1.4977769 17.631432 8
GCTTC 5461030 1.4972837 18.647177 6
ATTGC 8744530 1.4818534 7.3640027 7
CTGAT 8632665 1.4628967 9.322955 1
TCAGC 5759295 1.4583004 11.218716 9
CTGGC 4060405 1.4501357 6.6777897 8
CGGTA 6568600 1.4499446 56.02853 2
TTGTC 7895655 1.4488015 16.832018 8
GGTTG 6937920 1.4456419 24.511219 2
GACTG 6471560 1.428524 7.0908246 6
CGTCA 5638655 1.4277533 15.846208 2
ATCTT 10923870 1.4211355 9.440659 8
GCTTG 5940085 1.4197868 17.232046 6
GGTTT 8846615 1.4151379 7.896067 5
CTTCG 5152535 1.4127018 7.2651916 1
CGGTG 4529565 1.4102521 14.653435 6
GCTGT 5896410 1.4093477 5.925654 4
GTGAT 9539740 1.4093093 23.83424 1
CGTGG 4491100 1.3982761 8.04611 7
GTGCT 5841350 1.3961873 9.96714 8
AACCT 7744805 1.3903575 35.54132 5
TCGTT 7541670 1.3838474 11.950209 2
TATTG 12122555 1.3748462 14.443029 6
TGAAC 8694175 1.3606464 5.8050795 9
GTTCA 7990230 1.3540293 10.656473 1
CATCG 5337155 1.351411 6.9774756 1
GACTT 7972085 1.3509544 25.921003 3
ACGTG 6089075 1.3440948 23.971685 6
CGTGC 3756065 1.3414435 8.37713 7
TTTCA 10305475 1.3406858 9.572362 7
GTATT 11793685 1.3375484 58.28784 4
GAACC 5717625 1.3370306 46.52509 4
GCGGT 4280340 1.3326575 78.4937 1
ATCGG 6018170 1.3284433 5.332257 145-146
CTGTT 7210330 1.3230487 13.632573 8
AGGTT 8954885 1.3229084 17.405544 1
GTTCT 7180030 1.3174888 17.132797 1
TTCAG 7742590 1.312064 8.361531 8
GGTCA 5913095 1.3052491 8.377404 7
ATCGT 7701185 1.3050475 5.306316 1
CGTTC 4751385 1.302716 20.358469 1
CTTGT 7091255 1.3011992 13.53404 5
ATTTG 11472725 1.3011475 34.759594 6
TCTTT 9213395 1.2978683 13.4483385 9
TGGAA 9483120 1.2938057 13.000317 9
AGCAA 8933995 1.2912511 5.0059614 2
GGTTC 5385800 1.2873026 12.830805 3
TTGCA 7588775 1.2859986 6.415105 8
AGGTA 9410785 1.283937 8.519201 2
GTAAA 13214825 1.2782552 7.065095 8
TGGAT 8597085 1.2700504 15.880857 5
TTGTT 10285385 1.2630847 19.858788 8
ACGAA 8676880 1.2540897 14.400664 2
CTCAG 4942285 1.2514267 5.9491854 8
TGCCG 3495300 1.2483139 6.31727 9
GTGGC 3977510 1.2383732 9.481064 8
GTGCC 3448085 1.2314514 8.020232 8
GCTAC 4855405 1.229428 20.786367 2
GTGTT 7666250 1.2263223 19.951956 8
GATAT 11707450 1.2262255 34.303127 4
ATCCA 6814890 1.2234179 20.348206 1
CCATG 4824185 1.2215229 28.771585 3
CCATC 4204370 1.2211742 8.128848 6
TTCAC 6262205 1.2172929 12.251868 2
TGTCT 6607690 1.2124681 27.980932 5
CACCT 4171070 1.2115021 18.313904 4
CAACG 5168050 1.2085162 16.279633 1
TATCA 10046470 1.207037 5.122511 9
TGCAA 7682645 1.202341 7.13619 9
TCACA 6696520 1.2021679 9.215182 9
TGTTC 6549015 1.2017016 15.579551 9
TTATG 10571700 1.1989602 18.059479 6
TCAGG 5423855 1.1972549 11.188428 1
CATTG 7049205 1.1945626 6.4858384 6
ATCGA 7630665 1.1942061 15.382905 1
GGCCT 3342090 1.1935964 91.66084 1
GTATC 7040715 1.193124 5.586553 8
CAGGA 5816915 1.1858218 22.900707 2
TGGCA 5356915 1.1824787 8.871049 9
ATCCT 6023140 1.1708218 6.5948086 2
CGTGT 4883040 1.167134 15.83044 7
CGCCT 2848630 1.1670096 91.38596 1
TTTCT 8277990 1.1661001 17.660564 7
ACATG 7398615 1.1578901 15.31293 2
CACAG 4943830 1.1560837 6.067157 2
CGAAC 4916680 1.1497347 46.513397 3
ACACA 6918535 1.1470392 5.386081 4
CGTAT 6767465 1.1468188 43.934235 3
ACGTT 6712085 1.1374341 6.6217823 3
TGTCA 6678250 1.1317004 19.119194 9
TCCAT 5804500 1.128321 22.073366 2
GATAA 11591590 1.1212415 7.9270005 2
TTTGC 6037250 1.1077961 10.135803 7
CGATT 6527290 1.1061186 65.0976 3
TAAAA 16083330 1.1029872 7.6320453 9
ACGTC 4352235 1.1020212 18.200962 6
ATTCA 9168075 1.101502 9.499913 7
TACTG 6497110 1.1010042 6.236426 4
GATGT 7452465 1.1009554 22.166525 3
TTCTG 5993835 1.0998298 17.171362 2
TATCT 8433570 1.0971612 9.817195 7
ACTGT 6452995 1.0935285 16.689291 1
ATCGC 4317310 1.093178 5.61156 1
GACAA 7554905 1.091928 7.8255134 3
TCCAC 3749270 1.0889887 6.426042 1
ACACT 6050810 1.0862494 9.181489 3
GTAGC 4857405 1.0722175 5.5131135 8
CCACA 3983780 1.0686123 5.952292 2
CTGCC 2604960 1.0671843 5.5729995 8
TATTT 12227745 1.0646248 63.472187 5
CTACC 3647785 1.059512 23.845182 3
GCGTC 2961755 1.0577632 5.452297 145-146
GGACA 5184380 1.0568748 23.443909 2
CCTAA 5848600 1.0499483 46.495987 3
ATCTC 5397430 1.0491917 7.5425587 8
TCGAA 6690185 1.0470201 15.474088 2
AATTC 8714445 1.0470004 10.752355 1
GTTCG 4374805 1.0456568 20.79409 4
GTTCC 3811540 1.045033 20.34456 2
CATGA 6633420 1.0381364 15.347521 3
TCGAC 4083600 1.0340008 19.79294 1
CCTCT 3275775 1.0302502 8.422973 7
ATGTC 6073065 1.0291455 16.144539 4
ACCTG 4046320 1.0245612 20.112034 6
GACTC 4044760 1.0241661 13.465945 4
GATTA 9751560 1.0213677 40.235325 4
GCTTA 5987325 1.0146158 9.058069 6
ATTAT 12567975 1.0105635 30.763409 5
CTATT 7628465 0.99242145 29.053648 4
GTCAG 4483035 0.9895795 7.5297623 8
CACTC 3399405 0.9873692 14.88619 4
ACCTC 3395370 0.9861972 16.68182 6
TCTAA 8177840 0.98252976 6.082933 9
TACCA 5471175 0.9821924 14.740649 4
TGTAG 6628905 0.9792905 6.1323647 4
ACCAT 5450100 0.978409 16.89578 5
CCTAT 5021140 0.97604585 43.485367 3
ATTCT 7494935 0.9750499 13.036516 7
ACTCT 5004020 0.9727179 20.051228 5
GTGGA 5043250 0.97048765 8.7987585 8
GGCGT 3109790 0.9682139 80.093124 1
ACGTA 6166625 0.96508247 9.302122 6
TCTGA 5693040 0.96474606 6.9968214 7
GTTGT 6029060 0.96443117 8.367169 5
TCTTA 7399275 0.9626051 9.090049 9
ATGGA 7050580 0.96192825 6.6376348 4
GGTCT 4018000 0.96037394 12.790621 7
CCATT 4927900 0.95792115 18.17869 5
AACGC 4080355 0.9541655 5.085309 1
CTCTG 3476520 0.9531785 11.819297 6
AGGAC 4671310 0.95228153 23.320848 1
TCTCT 4518380 0.9510482 15.562952 7
CATCT 4892380 0.9510165 9.682181 5
CTGAC 3754465 0.95066106 6.6846223 1
CATTC 4889790 0.95051295 5.498444 6
CTGTG 3968365 0.9485104 20.432083 2
TCACC 3260175 0.94692934 18.312431 3
TAACG 6025380 0.94297755 12.843509 4
TGGCG 3026875 0.9423988 5.374302 9
GGATG 4894250 0.94181526 10.9492235 6
CACAC 3496040 0.93778056 8.714128 3
TCAGA 5983215 0.93637866 6.3258653 9
TATTC 7156875 0.9310702 13.158321 6
TTAAA 12537370 0.9310068 6.33873 8
AGTCA 5930875 0.9281873 6.714525 7
TTCTA 7133670 0.9280514 5.0624084 8
TGTTA 8156295 0.9250237 9.082173 9
ATTCC 4736420 0.9206998 17.393778 2
GAATT 8782700 0.9198904 12.351615 2
TCTGC 3343440 0.9166912 25.706728 3
CTCTA 4712505 0.91605103 7.7663956 3
TTCCG 3331635 0.9134546 24.545603 3
TTACG 5378750 0.91148627 18.997988 4
TTTGT 7401435 0.90892446 19.716845 7
GTACT 5309905 0.89981985 7.2410774 5
CGGTC 2491105 0.889675 11.270875 6
ATGTT 7836620 0.88876873 8.964319 8
GACAT 5637815 0.88232315 15.274709 1
CGACT 3481645 0.8815808 38.6831 2
ATTTC 6757315 0.87908965 29.017544 6
TGAGT 5941775 0.87778056 30.537104 5
TGTCG 3641975 0.8704973 12.230636 3
TAAAC 7845115 0.8704715 5.100715 9
ACAGG 4266780 0.8698151 12.554483 1
GTGTG 4169680 0.8688287 13.074623 2
TTATT 9975765 0.86855316 6.342241 8
TCTGG 3624875 0.86641014 8.438917 7
GTGCA 3900120 0.86090755 6.314366 8
GTCAC 3391850 0.858844 7.135279 8
GCCAT 3376575 0.85497624 5.5383463 4
GGTTA 5783145 0.8543461 5.2747416 6
ACTCG 3373470 0.85419 6.4750743 3
AGTCT 5008560 0.84875363 8.385969 7
AGACT 5418625 0.8480197 8.3134 3
TGTCC 3081755 0.8449434 10.599955 9
GTGTC 3534985 0.84492475 16.423334 8
GAGGA 4714320 0.8378122 6.0472617 4
CCTGC 2042035 0.83656865 5.088791 7
TATGC 4935665 0.8364008 5.033571 7
CCTAG 3299270 0.8354021 8.597897 4
CCTCA 2865560 0.8323121 5.7402887 7
CTCAC 2817640 0.81839347 5.346265 8
GCAAG 4014395 0.81836444 7.069144 3
TCTCC 2600905 0.81799966 6.132221 9
GGATA 5981780 0.81610924 11.15118 1
CGTCT 2971365 0.8146772 12.864392 7
GACAC 3470775 0.8116189 11.961972 2
TGTGA 5430195 0.80220467 12.693915 3
ATGAG 5863740 0.8000048 22.479038 4
ATATT 9922820 0.7978723 26.078573 5
CTGTA 4696905 0.7959404 7.0304694 3
GATCT 4668720 0.79116416 7.371862 5
CCTGG 2212820 0.7902881 5.0152016 7
GTAGT 5324505 0.78659105 9.915071 5
TCGCA 3087655 0.7818194 6.218369 9
ATTGT 6878210 0.7800732 12.446968 7
TGTTT 6303820 0.774133 18.601768 9
TGTGG 3700895 0.7711488 11.169027 9
CTACT 3959945 0.76976293 5.006116 1
ACCTA 4260115 0.76478136 8.592944 6
TCCTA 3932100 0.7643502 6.6008654 3
AACGA 5266465 0.7611745 6.2879577 2
GGATT 5132135 0.7581722 6.406428 5
CTAGC 2983690 0.75549453 6.713981 2
TGGAG 3913905 0.7531644 5.0423846 1
ATTAC 6227000 0.74814534 13.468631 3
TACGA 4754970 0.7441572 15.54429 1
GTCTG 3088120 0.738116 18.448147 6
GTCTC 2687215 0.73677 15.246053 6
CTGTC 2666935 0.7312097 11.345428 8
CTGGA 3311450 0.73096526 6.136008 8
GGAAT 5350005 0.7299146 16.058023 1
CGATA 4647135 0.7272809 51.270943 3
ATTAA 9777490 0.7260621 5.4976344 7
AGTGG 3704540 0.71287566 6.0501924 7
GGACT 3211380 0.7088761 11.258493 4
GTCAT 4171800 0.7069558 7.682185 3
CCGGT 1977795 0.70635104 31.73076 5
GATCC 2765875 0.700342 8.58395 1
CTAAT 5819080 0.69913566 31.126863 4
TAGAA 7165500 0.6931108 7.082899 9
TCGAT 4057625 0.68760765 5.55622 5
CTGAG 3103605 0.68508583 5.3042016 1
TCTGT 3716600 0.6819719 17.615488 7
AGCGC 2049070 0.6758405 10.146002 4
AGAGG 3778925 0.6715771 6.0503526 3
GCGTA 3040665 0.67119265 57.10236 2
GCATG 3034315 0.66979086 10.784682 2
ACGCC 1767615 0.668766 8.291027 2
ATCTG 3924730 0.66508716 6.1812873 6
CCTGT 2425725 0.6650758 11.373245 2
ACGAC 2843950 0.66503996 17.437548 1
TTCGA 3882730 0.65796983 5.0923934 8
AGTAA 6764895 0.6543607 5.2411137 7
ACGAT 4171435 0.6528334 6.836784 3
TTAGC 3818890 0.64715147 5.3545775 8
TGAGA 4735270 0.64604473 9.406743 1
TCTAT 4898595 0.63728034 5.2976255 7
CATGT 3749865 0.6354544 13.104672 5
CAGAG 3114700 0.634955 5.305847 4
CGCCA 1674200 0.633423 8.269916 3
AGTGA 4640010 0.6330482 5.9799614 3
AGACA 4345850 0.6281158 7.382052 1
TTGTA 5536435 0.6278996 6.7333694 8
GTCGA 2835920 0.62599736 7.3713994 4
TTTAT 7177910 0.6249542 5.3813143 7
TAGCA 3992070 0.62476265 6.6964936 9
GTGTA 4197570 0.62010854 6.772451 8
CTCTC 1950915 0.61357397 11.152454 8
TATTA 7601945 0.6112558 6.063775 6
AGAGT 4440210 0.60578895 5.0321093 5
ATAAC 5366785 0.59548306 9.109339 3
GAGTG 3091595 0.59492487 17.936522 6
TACGT 3459135 0.5861871 29.285805 5
CTAGT 3433600 0.58185995 12.400152 5
TGTGT 3633210 0.58118194 20.853355 5
TTCGC 2102910 0.5765676 13.564564 1
GCGCT 1611875 0.5756662 10.806313 5
CTAGG 2597030 0.57326514 12.527156 1
GGATC 2577920 0.56904685 9.521139 6
TTGTG 3524495 0.5637916 10.2513485 8
TCTCG 2044595 0.56057906 8.876345 9
CACAT 3114115 0.55904996 5.803477 5
ATTTA 6833470 0.5494644 12.477456 6
TGTGC 2279535 0.5448497 6.412718 7
GAGAC 2652695 0.5407718 10.836981 2
AGCGG 1872150 0.5383057 6.9686174 4
TAGGT 3600235 0.531864 7.981752 1
AGTGC 2368415 0.522801 5.6922717 7
TAATT 6449985 0.51862925 20.645096 5
TGTAA 4950540 0.51851416 9.170752 9
TATGT 4553320 0.51640224 10.442935 7
GTCTA 3027095 0.51297337 7.7499723 6
AGTGT 3458330 0.5109003 9.667879 7
GTGAG 2652160 0.51036304 8.447205 4
CGTAG 2256590 0.49811682 5.062818 2
TCGCC 1205640 0.49391934 20.458775 2
CGATC 1945110 0.49251765 11.075591 4
TAGTC 2827680 0.47918043 6.650641 6
AGCAT 3045575 0.47663525 10.367962 1
TTTCG 2591945 0.47560504 5.562492 7
TAGTG 3168405 0.46806964 8.536408 6
GAGTC 2080470 0.45924035 14.27267 6
ATGTG 3069090 0.4533978 8.101763 4
TCCGG 1251050 0.44680083 31.977406 4
TCTAG 2601315 0.44082043 6.772829 4
CTAGA 2588675 0.40513003 5.3196783 1
GAGTA 2958405 0.4036226 9.498394 6
GGAGT 2080490 0.40035492 5.059398 2
TTATA 4973160 0.39988095 6.4261503 6
CGCGT 1085850 0.3878012 7.780575 3
TAGCG 1653620 0.365018 6.7944145 3
TCGCG 941930 0.33640155 7.764271 2
GTACG 1515990 0.33463773 13.652014 4