FastQCFastQC Report
Tue 9 Jul 2019
SL_2_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SL_2_R1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 40326607
Filtered Sequences 0
Sequence length 100-150
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 8636640 5.208777 12.649278 145-146
CACCA 16644195 3.8821914 6.683168 105-109
ACCAA 19272465 3.6195621 5.985724 85-89
CAAGA 15815340 3.2936275 4.880386 30-34
CTTCA 23524810 3.134302 4.598967 70-74
CAGCA 11772510 3.0448115 4.9274898 100-104
CCAAG 11638175 3.0100675 11.95594 1
TCAAG 16721230 2.9330006 7.6900787 9
AGAGC 9920405 2.8451028 6.893913 140-144
GCTTC 15185750 2.786272 7.9094634 6
TCACC 13884055 2.727586 9.558089 3
CTGCT 14804175 2.716261 18.365877 4
TTCAA 24115600 2.587124 5.999432 8
GAAGA 11192745 2.5846977 7.439438 3
GCAGC 7199415 2.564255 5.4509826 95-99
GTCAA 14337125 2.5148146 9.633077 8
AAGAG 10838880 2.502981 7.4456244 4
TTCTT 32734305 2.4912553 9.450007 8
AGCAA 11806880 2.4588447 8.04595 2
TACCA 15111740 2.390458 10.76259 4
TAGCA 13339525 2.33983 6.797764 1
TCTTC 20833140 2.337854 9.060776 9
ACCTT 17086445 2.2764935 6.4179707 5
CTTCT 20237615 2.2710252 7.164786 5
GGAAG 7134510 2.2688715 6.8378205 135-139
GCTTG 11141915 2.2668579 22.317055 6
ACGAC 8738305 2.260053 22.519379 1
GAAGG 6817180 2.1679564 13.286472 3
GGCAA 7453850 2.137712 6.410872 40-44
TCAAC 13452035 2.1279173 5.0288196 9
ACTGC 9761760 2.1265101 5.8408437 15-19
TGAAG 10907865 2.1215866 5.7713804 9
CAAGG 7389450 2.1192424 13.281775 2
AGCAT 12003720 2.105522 10.614163 1
TGTTG 15258475 2.1053684 30.884195 9
GAGCA 7088625 2.032968 6.3042507 140-144
TCTTG 16263815 2.023775 10.752774 9
TTGAA 16999850 2.022281 6.310595 8
CGATA 11524505 2.0214648 59.254154 3
AGCAG 7011600 2.0108778 5.29011 20-24
TGCTT 16108850 2.0044918 24.092472 5
CTGCA 9107040 1.9838852 5.966165 8
GTTGT 14336400 1.9781404 9.844233 5
CCGAT 9063260 1.9743482 151.26183 2
ACCAT 12288425 1.9438506 13.620887 5
GTGTT 13995480 1.9311001 29.754784 8
GCCGA 5340430 1.9021301 247.48438 1
AGAAG 8227325 1.8999046 13.060231 2
TCAAA 14903040 1.8982183 7.1277843 9
GACGG 4753895 1.8775469 5.376059 55-59
TGGCA 7663815 1.8512349 23.381266 9
GATAA 13099720 1.8501652 10.592905 2
AAGGT 9502845 1.8483095 10.380373 1
AACTG 10404915 1.8250821 11.258571 2
CAACG 7029910 1.8181977 21.821814 1
CAACT 11421580 1.8067284 11.473385 5
TCAGC 8291070 1.8061337 5.6626997 9
GCAAG 6260390 1.7954361 11.073823 3
AGTCA 10198190 1.7888216 6.1665654 7
CACCT 9081030 1.7840098 9.56065 4
AGCAC 6835210 1.7678412 6.9620614 145-146
TTGTG 12802900 1.7665474 10.26183 8
TTCAC 13239595 1.7639624 6.476526 2
GTCAC 7976315 1.7375673 8.024655 8
TTCTG 13896845 1.7292428 12.43973 2
GATGA 8858630 1.7230093 12.094382 3
TTGTT 20414080 1.7227494 25.327286 8
CATCT 12892515 1.7177199 6.959056 5
TCACG 7876635 1.7158527 7.8672433 9
TCTGC 9173335 1.683118 18.357775 3
ATCTT 18448775 1.6669967 6.1287284 8
TTTCT 21884070 1.6654947 7.127111 7
CTTGT 13107850 1.6310651 10.954947 7
CGTTC 8879395 1.6291859 11.52902 1
ACGTC 7455235 1.6240548 23.458672 1
ACTTC 12116295 1.6143011 8.584337 4
TGTGT 11670630 1.6103166 22.517044 5
TGCAG 6660365 1.6088462 12.497606 9
TCTTT 21113115 1.606821 12.670048 9
CCATG 7336090 1.5981 22.054117 3
CGAAG 5550860 1.5919479 11.931837 2
CTGTG 7789275 1.5847526 18.546085 2
ACCTG 7256370 1.5807338 18.977661 6
GGTTT 11452335 1.5801963 6.3320103 5
ACGTT 10673215 1.5768383 8.991911 3
CTTGG 7747325 1.5762179 14.101325 3
AGGTT 9617845 1.575603 15.823174 1
TGTTT 18665450 1.575182 25.586725 9
TGTCA 10555585 1.5594596 29.959518 9
TACTG 10495935 1.5506473 8.254682 4
ACGAA 7437515 1.5489016 18.987446 2
AGGAC 5399700 1.5485963 35.234024 3
GGTCA 6295645 1.5207462 6.8085594 7
ATAGC 8535770 1.4972234 5.1621103 2
CTTCG 8103680 1.4868581 5.5530176 1
TGAAC 8418440 1.4766427 5.503236 9
TGACG 6086465 1.4702176 7.5844026 9
TTCCA 11013485 1.4673692 8.375002 3
TGTGA 8913305 1.4601847 14.934998 3
CGTCA 6663200 1.4515171 29.869154 2
AACGA 6945270 1.4463888 8.885527 2
ATAAC 11219790 1.4290782 9.557109 3
CGAAC 5511205 1.425404 41.57417 3
ATTTC 15645255 1.4136759 15.065229 6
CGGAA 4927360 1.4131323 6.0724382 135-139
GACAA 6778395 1.4116364 10.453251 3
TGAGA 7249170 1.4099683 29.656094 1
ACGGT 5827425 1.4076453 5.6975107 55-59
GTTCA 9512265 1.4053217 7.1808186 1
GAACC 5430510 1.4045331 41.55619 4
TGGAA 7219650 1.4042265 7.2548547 9
TGAAA 9895640 1.3976303 7.7726216 9
GACTT 9428250 1.3929098 22.356922 3
ATTTG 13741800 1.3768543 39.91564 6
TGCTG 6766360 1.3766373 14.862913 9
TCGCC 5060270 1.3690157 17.695433 2
CGCCT 5000020 1.3527157 57.08253 1
ACTGT 9145420 1.351125 15.731268 1
AACCT 8540970 1.3510576 25.221748 5
GTTGC 6615950 1.346036 19.445114 3
TTGCT 10755340 1.3383322 11.893839 4
TTGGA 8151485 1.3353827 11.339226 4
GTGAT 8148330 1.3348657 22.82873 1
AGCTA 7567655 1.3274105 11.986924 1
AATTG 11144120 1.3256907 17.040857 6
CCTTG 7191745 1.3195369 5.0402274 6
CGATT 8725030 1.2890176 52.7455 3
GTAGC 5317175 1.2843915 5.169427 8
TACGA 7316820 1.2834126 15.983158 1
TTGTC 10284895 1.2797927 19.8676 8
TGACT 8590855 1.2691945 13.380949 5
GGATA 6520315 1.2682055 14.572095 1
ACTGA 7227880 1.267812 5.2634573 4
TTGGT 9156455 1.2634101 20.644346 5
CATGT 8539345 1.2615848 20.29467 5
TGGAG 4689570 1.2561071 9.033749 9
ACACG 4833640 1.2501602 5.5957165 145-146
TTTGC 10036955 1.2489407 11.627784 7
ACTGG 5170075 1.2488588 15.505134 3
AGGAT 6362295 1.2374705 6.272022 5
GCTAC 5654265 1.2317299 14.820936 2
CTGAC 5646425 1.2300221 6.544706 1
ATCCA 7767970 1.2287803 15.999171 1
TCCGG 4061715 1.2184885 23.766773 4
GAGAA 5259065 1.2144557 13.020268 1
TTACG 8219580 1.2143432 15.066244 4
TGAGT 7404710 1.2130454 32.604984 5
TTTGT 14372410 1.2128916 20.667318 7
GTGGC 3637320 1.2099588 15.490069 8
GAGTG 4508295 1.2075526 31.425837 6
ATCTG 8154585 1.2047411 7.3970046 6
TCAGG 4983580 1.20381 10.970755 1
GCCTA 5523915 1.2033343 105.658356 2
AGTGA 6185620 1.2031071 7.6441016 3
AGTGT 7342475 1.2028501 17.351395 5
AACGT 6852680 1.2019995 13.069868 5
TTTGG 8686690 1.198592 7.6369553 7
CGCCA 3722665 1.1957511 8.188997 3
CTGTT 9591470 1.193507 15.782471 8
AGATC 6795200 1.1919173 5.473463 145-146
GCCAT 5457815 1.188935 5.5481896 4
GTGAC 4901730 1.1840386 22.02144 4
ACGAT 6725745 1.1797346 7.4807796 3
TACGT 7969500 1.1773971 22.72864 5
TGCAA 6683500 1.1723245 7.988786 9
GGTGT 5144670 1.1606444 24.23508 7
CGGTA 4785400 1.1559386 58.497936 2
GTTGG 5111530 1.153168 9.224283 4
TTGCA 7767555 1.1475619 9.824342 8
CCATT 8610805 1.147251 11.58669 5
GACTG 4735160 1.1438029 9.238928 6
TCGAT 7738835 1.1433191 5.564949 5
AGTGG 4233010 1.1338171 8.6557665 7
TGTGG 4988485 1.1254088 8.592299 9
CAGGA 3922770 1.125023 35.22717 2
CACTG 5132560 1.1180812 7.6516314 2
GTCTG 5489315 1.1168184 17.835613 6
CTAGG 4601530 1.1115237 15.615141 1
GGTTG 4909095 1.1074984 21.555563 2
TTGGG 4905705 1.1067336 5.068284 8
CGTTG 5411915 1.1010712 14.61838 4
GTTCG 5402325 1.0991201 21.162151 4
TGGAT 6700070 1.0976107 18.94461 5
TATCA 10228295 1.0972927 6.962222 9
GAGAT 5639840 1.0969526 8.316692 5
TCTGG 5363745 1.0912709 8.520661 9
TCTGT 8761215 1.090195 16.860107 7
TGCCA 4994040 1.0879059 20.434738 9
GTCTT 8722425 1.085368 16.559998 8
GATTA 9096170 1.0820692 42.46231 4
TTATC 11969650 1.0815551 7.736114 6
ATTGA 9050875 1.0766809 6.1908727 7
GGACA 3753700 1.0765349 29.636578 2
TAGAG 5515880 1.0728424 6.3629966 2
GTTCT 8602650 1.0704639 12.435944 1
GTATT 10646870 1.066759 50.471943 4
GAGTC 4411215 1.0655522 18.706097 6
GGATG 3968755 1.0630361 18.325823 6
GGAGT 3962825 1.0614476 7.5480027 2
GTCGA 4375335 1.0568852 9.175965 4
TTCGT 8489900 1.0564339 12.84372 5
CAGTA 5994440 1.0514594 6.453208 1
ACGTG 4348340 1.0503644 30.057953 6
CACAC 4501860 1.0500407 8.682826 3
GTCCA 4810315 1.047883 5.3874664 8
GGCGT 3142520 1.0453631 87.11078 1
TGTTA 10419860 1.0440137 10.751446 9
TGATG 6365355 1.0427774 34.217182 2
GAATT 8752595 1.0411978 13.153828 2
GCGGT 3116235 1.0366194 80.56002 1
AGAGG 3243635 1.0315202 10.729707 1
GGTTC 5060480 1.0295707 12.744018 3
TAGGT 6254905 1.0246835 10.402059 1
CATCG 4696865 1.023169 5.7753153 1
GTGAA 5249230 1.0209786 7.7571855 8
CCTGC 3771920 1.0204629 5.191333 7
GTCAG 4217150 1.0186747 5.4247766 8
GTTCC 5542245 1.0168877 11.527693 2
GTCAT 6862385 1.0138342 16.85177 3
GATCG 4181275 1.010009 7.0068374 145-146
GTGGA 3747375 1.003739 8.348378 8
TGGTG 4448145 1.0035074 27.929956 6
ATCGG 4151935 1.0029218 7.49339 145-146
AGAGT 5120130 0.9958686 20.696672 3
ACTTG 6732570 0.99465567 12.838405 4
CTGGC 3314070 0.9941997 7.9061847 8
ACAAC 5284055 0.9923984 9.4236145 4
TGGTT 7183495 0.99118054 8.78635 5
CTGCG 3296615 0.9889633 5.25363 8
TGTTC 7917070 0.9851542 17.947655 9
TTCCG 5358830 0.9832348 14.542131 3
AGGAG 3090265 0.9827465 10.709676 3
CATGG 4047965 0.9778073 24.452682 4
CTAGC 4484960 0.9770075 5.6191673 2
CTGAT 6608535 0.976331 10.269973 1
AGTAC 5540375 0.9718138 6.4590516 2
CAGGT 3997370 0.9655858 5.5839334 4
TATCT 10639380 0.9613544 5.410837 7
TCCAG 4373670 0.9527639 5.628788 9
TTGGC 4674400 0.95102143 13.168267 8
GAGGA 2972460 0.94528276 10.740759 2
TTCGC 5149010 0.94473714 11.998237 1
CAGAG 3287325 0.94278187 9.758145 4
CATTG 6380440 0.94263273 7.0450773 6
GACAT 5334390 0.9356829 17.674059 1
GTGTG 4144310 0.9349618 18.900543 6
TATTT 15214610 0.9323533 43.369926 5
GGCCT 3082275 0.9246627 82.18247 1
GCGCT 3062250 0.91865534 8.686833 5
ATTGG 5606700 0.91849405 18.113487 4
CGTCT 4997045 0.91685474 8.362871 7
CTGTA 6166560 0.9110344 7.1956887 3
CTACC 4631220 0.9098244 13.366759 3
TCCAT 6822720 0.9090175 13.484201 2
AGACA 4363470 0.90871555 8.719359 1
GTGTC 4465950 0.90861166 22.529917 8
ATTTA 12427120 0.9041536 7.988503 6
CCTAA 5673485 0.8974631 43.36831 3
GCGTA 3697420 0.89313126 63.257404 2
ATGGT 5450260 0.8928659 16.32764 5
TGCGA 3669930 0.88649094 6.7798777 9
GTGTA 5371620 0.87998307 6.668933 8
ATGAG 4498005 0.87486494 31.68317 4
GATAT 7331645 0.8721634 40.16363 4
GGTAT 5311175 0.8700809 39.673058 3
GCACA 3359885 0.868992 5.6297474 145-146
CGACT 3975895 0.8661123 32.976208 2
ATTGT 8637620 0.865443 18.91157 7
TGGTC 4246675 0.8639994 13.360211 6
GGAAT 4389815 0.8538219 21.50375 1
ATGTT 8494295 0.8510826 13.623013 8
CTCTT 7582490 0.85089207 6.328633 8
CCTGT 4630255 0.8495563 8.945142 2
AATTC 7896410 0.84712785 8.506957 1
GGATC 3486530 0.84218967 10.481046 6
TGCCG 2805240 0.841554 8.46919 9
CCACA 3588805 0.83707434 5.396748 2
ACATC 5280280 0.8352637 5.3561554 6
TCGTT 6664440 0.8292843 10.334728 2
CTGCC 3064905 0.8291856 7.058114 8
AGACT 4721190 0.82812405 8.11274 3
CGCGT 2747875 0.8243449 8.6462145 3
CTGTC 4468610 0.8198978 12.082596 8
TCATG 5478570 0.80939233 12.509154 4
TGTAG 4907025 0.80387264 7.9783077 4
GATGT 4899325 0.8026113 24.048203 3
AGGTA 4124995 0.8023142 10.558803 2
TCGAA 4570930 0.8017676 12.207909 2
GGTAC 3297805 0.7966022 13.106072 3
CACAG 3055935 0.79037917 8.807078 2
TGTCC 4306150 0.7900897 14.568037 9
ATTGC 5342075 0.7892269 12.700947 7
TCGTC 4254445 0.78060293 6.4765725 6
GGATT 4754155 0.77882946 6.113228 5
GTACG 3222375 0.7783816 12.889375 4
AGTCT 5253280 0.7761085 9.001215 7
ATTCA 7206515 0.77311575 6.0106945 7
TGTGC 3796905 0.7724923 11.426042 9
TAATT 10567385 0.7688459 19.741606 5
GACAC 2968745 0.7678286 10.792464 2
CGGTC 2558760 0.76761156 7.4785004 6
GTGCA 3174335 0.76677734 11.169439 8
TGGTA 4670620 0.76514465 6.686962 6
CATGA 4315825 0.75702065 17.677237 3
AGTGC 3131665 0.75647014 11.182555 7
CGTGT 3713915 0.7556077 18.109312 7
CCGGT 2514365 0.7542934 23.448563 5
GAGTA 3858705 0.75052065 8.377389 6
ATTAC 6986945 0.7495603 10.944342 3
AAGCG 2601030 0.74595726 7.209852 3
AGTAG 3791850 0.73751736 5.4584637 4
GATCC 3380625 0.7364382 7.092648 1
ATGTC 4982085 0.73604274 11.428341 4
ATGGA 3782030 0.7356073 9.23897 4
ATTCT 8098240 0.7317418 7.4233246 7
GCATG 3023565 0.73035806 11.479707 2
TGCAC 3321445 0.7235464 9.627473 9
TGTCT 5764255 0.7172705 15.552005 5
ATGTG 4371420 0.7161295 11.383587 4
TGGGA 2668255 0.7146954 6.0413113 9
ACAGG 2481685 0.7117298 22.20142 1
ATTCC 5306685 0.70703024 10.563075 2
CTGGT 3454465 0.7028218 13.058228 4
TAGCG 2892540 0.6987083 7.117604 3
GTGCT 3430560 0.6979583 10.962017 8
TGTCG 3416720 0.6951425 13.705357 9
TGGCT 3413585 0.6945047 6.1131864 9
TGTAA 5828950 0.69340456 6.668902 9
ATCGA 3951590 0.69313174 12.22175 1
ACCTC 3472015 0.6820932 7.3265977 6
CCTAG 3084725 0.67197907 7.087759 4
TTGCC 3657265 0.6710327 12.091497 8
TTGTA 6694335 0.67073625 5.735947 8
GATGC 2768165 0.6686648 5.5333867 7
ACATG 3734875 0.6551186 17.688665 2
ATGCC 2930620 0.6384088 5.448507 8
TGGCG 1918490 0.63818806 9.329256 9
GTGAG 2380575 0.63763994 10.5206585 4
GGAGA 2002960 0.63696855 10.684807 4
ATATT 8746600 0.63637197 24.196033 5
GTACT 4299210 0.6351561 8.259173 3
TCGAC 2905900 0.63302374 14.0498085 1
ATCGC 2893940 0.63041836 6.283195 1
GGTGC 1880820 0.6256571 13.231578 7
ACAGA 2965155 0.61750907 7.0893617 3
GCTCT 3358815 0.6162733 6.9439297 2
GAGAC 2138360 0.6132667 13.260596 2
ATCGT 4137230 0.6112256 6.0955133 1
AGCTC 2788005 0.6073414 8.252411 1
CTTGC 3283960 0.602539 6.860634 7
CGATC 2741845 0.59728587 9.287729 4
TTGCG 2920890 0.5942643 8.410032 8
AGCGC 1666660 0.5936234 10.494456 4
GTGGG 1573005 0.58022493 5.732212 8
TATTG 5790395 0.58016634 17.626865 6
GTAGT 3499445 0.57328176 12.416947 5
GGTGA 2128900 0.5702286 6.365766 7
ACTCT 4258420 0.5673658 11.563538 5
TGCGC 1883215 0.56495243 7.027792 9
GTGCG 1693665 0.56339973 9.315287 8
TAACG 3183685 0.55843675 13.158263 4
ATTAT 7647225 0.5563853 25.822971 5
TATTC 6139745 0.55477583 7.6514564 6
CGTAT 3728210 0.5507979 38.674706 3
GTGCC 1801075 0.5403109 13.024493 8
ATGGC 2235440 0.5399823 6.082039 8
GAGAG 1669775 0.5310112 33.835453 2
ACGCC 1647655 0.5292405 8.196439 2
TCTCC 3183295 0.5267287 6.084786 9
CCTAT 3953295 0.52671283 28.12003 3
CTCTG 2865540 0.5257675 8.969984 6
CTAAT 4789360 0.5138031 29.408304 4
GATCT 3323035 0.4909382 6.258214 5
GGTCT 2397715 0.48782268 10.080959 7
GGACT 1976565 0.47744966 10.988595 4
GTCGT 2330315 0.47410995 5.0939536 4
TAGTG 2780085 0.45543572 12.135123 6
TTCTC 4024300 0.45159897 6.3115697 8
GTCGG 1357395 0.4515391 5.661765 8
TCTCA 3334675 0.4442917 11.397227 9
GTCTC 2398900 0.44014868 12.547562 8
CGTGG 1292200 0.42985192 7.5505447 7
ACACT 2708155 0.42839086 7.6456776 1
ACGTA 2428725 0.42601243 6.275788 6
TGCGG 1254750 0.41739413 9.063834 9
CACAT 2622845 0.41489604 5.846931 5
CGTGC 1366120 0.40982723 10.834593 7
AGCGG 1033785 0.40829253 9.906546 4
AACGC 1566820 0.40523827 6.5951023 1
GGTGG 1093250 0.40326062 9.574074 7
CTAGA 2283460 0.4005321 5.744793 1
CACTC 1986135 0.3901853 8.191855 4
TAGTC 2633750 0.38910466 5.574683 6
GACTC 1755320 0.3823804 10.066741 4
TGCCT 2011705 0.36910638 5.0921707 9
ACACA 1916920 0.3600168 6.9948244 2
TCTCT 3096570 0.34749097 6.0349097 7
TCTAG 2343400 0.34620896 5.5892305 4
CTATT 3729740 0.3370123 19.065737 4
CGGTG 990075 0.32934967 18.428736 6
TCTCG 1513485 0.2776933 6.5760756 9
TTATG 2769700 0.277509 19.821943 6
TCGTG 1360560 0.27681023 8.861387 6
TATGC 1763660 0.2605594 7.2106633 7
CTCTA 1688930 0.2250227 5.044276 3
TCGCG 748855 0.2246517 8.644912 2
TATGT 2083285 0.20873395 13.560839 7
CTAGT 1391380 0.20555954 10.22708 5
TGGGG 369570 0.13632107 5.227976 9