FastQCFastQC Report
Tue 9 Jul 2019
SL_1_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SL_1_R2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 44301365
Filtered Sequences 0
Sequence length 100-150
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGGTG 18263000 3.302279 26.223263 6
TTGGT 21985450 3.2370062 25.300642 5
TGCTG 14570070 3.134292 17.76105 9
TGAAG 25693495 2.986584 10.075157 9
TCTTG 16338425 2.8619003 21.051586 9
CTTGA 17742380 2.761391 5.4536557 7
CCTTC 8917575 2.715169 6.364258 65-69
TGGCT 12270795 2.6396751 13.279775 9
CTTGG 12025750 2.5869615 13.563402 3
TGATG 19660085 2.5719662 30.366884 2
GCTCT 9890030 2.5311131 11.778601 2
TCAAG 18302230 2.5309975 5.7830706 9
CGTCG 8039165 2.5267272 8.586879 145-146
GTCGT 11554135 2.4855084 8.053203 4
TTGAA 25856295 2.447284 12.66847 8
TCGTG 11356845 2.4430673 9.849195 6
CTGGT 11320455 2.4352396 20.656408 4
TTGCT 13753260 2.4090729 21.531206 4
GCCGA 8349920 2.3318543 212.42297 1
AAGGT 19620680 2.2806869 8.022064 1
ATGCT 14576985 2.2687347 8.067756 15-19
TTCAA 19843150 2.2344198 8.609989 8
TTGAC 14335065 2.2310824 6.182443 8
TGGTC 10357850 2.2281654 12.923841 6
GGTGA 13512200 2.170898 7.970139 7
TGCTA 13885365 2.1610923 12.331476 9
CTGCT 8443765 2.1609764 29.831657 4
CTCTT 10216935 2.1291244 9.959672 8
CTGAA 15375370 2.1262445 8.296901 8
TGAAA 25006215 2.1029925 15.675279 9
CTTCT 10075560 2.099663 14.959993 5
GTTGC 9584675 2.061841 26.452412 3
ACTTG 13246415 2.0616472 11.014861 4
TGGTA 15720080 2.0565279 9.079067 6
ACAAC 13718140 2.0053527 10.152616 4
TCTTC 9611875 2.003035 10.946029 9
GGTGG 8774540 1.9484959 8.930663 7
TGCCA 8523640 1.9382544 12.856765 9
GTCAA 13964740 1.9311705 7.6230617 8
GTGGT 10623190 1.9208639 6.9657016 8
TTGCC 7460695 1.9093835 12.556316 8
TTTGG 12924280 1.9028937 10.638366 7
GCTGA 9936555 1.8992641 5.019465 20-24
CAACT 11512315 1.8940285 15.792485 1
TGGTT 12720775 1.872931 12.281924 5
GGGGG 6859305 1.8706337 6.580697 90-94
CAGCC 5591900 1.857867 6.6314855 60-64
CCAAG 9152015 1.8491582 10.080241 1
GTCTT 10398475 1.821436 13.151495 8
CCTTG 7116925 1.8214043 8.002492 6
GGCTC 5738250 1.8035444 6.463238 10-14
TTGGC 8376045 1.8018423 12.630263 8
ACTGG 9371995 1.7913544 18.356031 3
TGTTG 12075935 1.7779884 22.889318 9
ATGGT 13406910 1.7539153 16.17485 5
GAAGG 12172760 1.7376974 9.67028 3
CTGGC 5482400 1.7231302 8.000779 8
TGCAG 8972065 1.7149123 8.680834 9
CGTTG 7964700 1.7133545 16.450674 1
TCAAA 17109265 1.7118167 7.555702 9
CTCAA 10380790 1.7078679 5.876143 8
TCGTC 6661490 1.7048465 7.6049385 5
GCCTA 7482970 1.7016085 128.2075 2
TGCCG 5390345 1.6941973 12.590506 9
TTTGA 15895030 1.693198 8.673598 7
GTTGG 9317050 1.6846902 13.833374 2
GTGAA 14372065 1.6705933 11.964491 8
AACGT 12045470 1.6657563 13.365647 5
GGTGC 6291105 1.6620275 11.49883 7
TCAGC 7306265 1.6614261 13.171373 9
TGCTC 6473390 1.6567068 8.208205 9
CTTGC 6471690 1.6562718 9.041976 7
ACCTT 8915265 1.6507692 11.10084 5
TGACT 10544910 1.64119 14.427054 5
AATTG 17278270 1.6353786 14.305139 6
TTCTT 11450045 1.6331196 15.84698 8
AACTG 11780335 1.629091 13.269784 2
GGTAA 14013455 1.6289089 5.547008 7
GCTGT 7544175 1.6228918 7.820112 4
CTGCA 7120410 1.6191633 6.028216 8
TCTCA 8707005 1.6122074 14.2085495 9
TTATC 12691930 1.6084597 10.995464 6
GACTG 8367480 1.5993525 8.2181225 6
GATGA 13736425 1.5967073 9.364075 3
AGCTC 6984435 1.5882429 10.473355 1
CTCGT 6162005 1.5770154 8.442964 4
TTCCA 8450520 1.5647161 15.4784565 3
GGTTT 10622365 1.5639734 6.971839 5
ATCGG 8132120 1.5543659 7.5272646 145-146
TTGGA 11839365 1.5488461 10.119457 3
TTCTC 7415510 1.5453308 11.328942 8
ACTTC 8339975 1.5442473 13.632353 4
CGAAG 9072000 1.5407219 11.499418 2
CGTGA 8050585 1.5387815 7.9666653 7
GGTGT 8462995 1.5302618 17.47333 7
TGCTT 8727225 1.5286937 40.48109 5
GGTTG 8429405 1.524188 22.267658 2
CCGAT 6679825 1.5189754 173.26987 2
GCTTC 5927595 1.5170239 13.164022 6
GCCTT 5918330 1.514653 5.4513836 60-64
GTGAC 7923045 1.5144036 18.156385 4
CTTCG 5881415 1.5052053 5.8406463 1
GGTCA 7873460 1.5049258 6.986392 7
CGTCA 6617595 1.5048245 14.851268 2
TCAAC 9130470 1.5021627 7.313337 9
ATTGC 9613390 1.4962101 12.352064 7
TTTCA 11703645 1.4832133 7.9807777 7
TTGTC 8442420 1.4788061 17.785393 8
GGTAC 7697315 1.4712577 13.322723 3
ATTGA 15504575 1.4674994 9.127148 7
GTGCC 4610790 1.4491813 11.179417 8
CATCG 6345305 1.4429063 8.461922 3
TTCGT 8177900 1.4324718 15.1743145 5
GTGCT 6655865 1.4317998 14.5708685 8
GCTTG 6631075 1.4264671 24.070505 6
CATGG 7461125 1.4261125 24.243744 4
AGCAA 11594810 1.4246988 5.2752967 2
AGCTA 10290975 1.4231288 11.649308 1
GTTCA 9139115 1.4223946 10.311273 1
ATTGG 10734150 1.4042599 18.562489 4
GCGGT 5307210 1.4020953 70.690094 1
CAAGG 8252790 1.4015933 8.481668 2
TAAAA 22968820 1.3975477 7.6110477 9
AACCT 8484990 1.3959671 36.227757 5
TATCG 8898330 1.3849194 5.922902 9
GTGGC 5225065 1.3803937 11.387115 8
GTGTT 9313195 1.3712193 21.215113 8
CGTGG 5185695 1.3699927 9.249824 7
TCGTT 7808280 1.3677278 12.011569 2
TATTG 12839150 1.3676742 19.018099 6
CGTTC 5339255 1.3664526 21.402937 1
CTGTT 7777255 1.3622934 14.710012 8
GTTCT 7758685 1.3590405 20.358994 1
CGGTG 5125805 1.3541707 17.099293 6
GTAAA 16068000 1.3512995 6.4066343 8
GTGAT 10263685 1.3427129 23.502405 1
GCTAC 5868040 1.3343775 19.08075 2
CGGTA 6976005 1.3333873 51.23585 2
CACAG 6574495 1.328372 6.4481173 2
TTCAG 8527945 1.3272732 8.224133 8
GACTT 8523920 1.326647 22.481873 3
AGGTT 10139760 1.3265009 16.22024 1
GGTAT 10130225 1.3252535 35.07824 3
TCGTA 8458200 1.3164183 6.1857057 1
GAGCG 5598560 1.3141934 5.7124867 140-144
CTCAG 5764790 1.3108987 5.6195846 8
CTGAT 8413025 1.3093873 8.144706 1
ACGTT 8349120 1.2994415 5.6014833 3
ATCTT 10228945 1.2963233 6.114016 8
CACCT 4789240 1.2956519 17.943445 4
TGGAA 11136135 1.2944523 13.817023 9
CGCCT 3459245 1.2934954 86.49736 1
CGTGC 4099805 1.2885778 12.125444 7
GAACC 6362610 1.2855608 45.13697 4
ATCGT 8250785 1.2841367 5.801961 4
TGAAC 9261550 1.2807707 6.019379 9
ACATG 9256240 1.2800364 16.62859 2
TCACA 7746265 1.2744305 6.4429336 9
CTACC 4705120 1.2728946 22.691496 3
GTATT 11888910 1.2664511 58.215305 4
CCATG 5547060 1.2613875 28.834974 3
TGCAA 9110035 1.259818 10.755454 9
CTGTG 5847010 1.2578 20.40528 2
CGAAC 6210850 1.2548978 45.129665 3
GCGTC 3966880 1.2467991 7.900269 145-146
ACTGT 7987610 1.2431766 17.034288 1
ACACA 8486850 1.2406294 5.844245 2
GTCAG 6482170 1.238996 7.388588 8
CCTGA 5445440 1.2382793 5.3240423 7
TTGCA 7940925 1.2359107 9.576971 8
CGTGT 5725545 1.2316709 17.36671 7
AGGTA 10585765 1.2304779 8.110036 2
ATTTG 11503330 1.2253777 40.8966 6
TCCAT 6597075 1.2215283 23.470247 2
GATCG 6346885 1.2131377 7.258572 145-146
TTCAC 6510360 1.205472 12.288699 2
GGTTC 5585745 1.2015972 11.739886 3
CTTGT 6853015 1.2004 12.668454 7
GGCCT 3776420 1.186937 104.27503 1
ATCCT 6391955 1.1835479 7.228883 2
TTATG 11097100 1.1821045 20.592905 6
ACTGC 5185925 1.1792662 5.1552305 7
ACGTG 6144725 1.1744971 28.688063 6
TAACG 8443945 1.1677049 13.585463 4
CCTAA 7073615 1.163765 54.78831 3
TTGTT 9691510 1.1618929 22.718916 8
ACCTG 5058565 1.1503049 24.558971 6
CATTG 7384430 1.1492988 7.6765842 6
AATTC 10203855 1.1489959 11.652301 1
TATGG 8732910 1.1424543 5.0513124 7
TCTTT 7997350 1.1406618 8.844889 9
CCTAG 5014540 1.1402938 8.874335 4
GCTTA 7310600 1.1378081 7.899003 6
TGACG 5927220 1.1329234 5.2860103 9
CTCTA 6118010 1.1328236 8.51726 3
TTAAA 16465530 1.1275401 6.1235886 8
TCAGG 5893650 1.1265068 9.960506 1
GATAA 13372895 1.1246444 8.248461 2
AGTCA 8124475 1.1235257 5.651421 7
ATCGA 8085130 1.1180845 15.313094 1
TGGAT 8544790 1.1178441 14.883391 5
ATCGC 4913455 1.1173072 5.1831737 1
CCACA 4643225 1.116126 5.6298494 2
GTTCG 5180600 1.1144428 19.106722 4
ATTAT 14436350 1.1126075 29.10143 5
CCGGA 3975450 1.1102107 5.2710323 80-84
ACGAA 9033045 1.1099249 13.819299 2
CCATC 4081480 1.1041789 5.551778 6
CTGCC 2951365 1.1035867 7.783243 8
CAACG 5461305 1.1034528 14.56077 1
GATAT 11642405 1.1019472 36.11663 4
TGTTC 6260390 1.0965935 16.281446 9
ACACT 6638930 1.0922498 9.332307 3
CGCTG 3432890 1.078965 5.3505898 6
TTCTG 6121820 1.072321 20.401535 2
GACAA 8721360 1.071627 8.537074 3
ATTCA 9482675 1.0677881 8.514351 7
TACCA 6475350 1.0653371 13.800391 4
TGGCA 5561580 1.0630352 9.49752 9
ATCCA 6427390 1.0574466 20.844248 1
GACTC 4624580 1.0516179 12.09243 4
CAGGA 6191470 1.0515139 19.693945 2
TCGAC 4618850 1.0503148 16.731035 1
TTACG 6738475 1.0487636 20.203815 4
TTTCT 7332555 1.045842 12.071381 7
TACTG 6636515 1.0328947 5.6512084 4
ATTCT 8116190 1.0285721 10.164591 7
TGTAG 7861130 1.0284065 6.5031495 4
TGTCT 5840195 1.0229906 27.402018 5
TGTCA 6561370 1.0211992 17.531998 9
TCTTA 8036855 1.018518 6.0200286 9
TCCAC 3742710 1.0125301 6.3308644 1
CGTAT 6460650 1.0055234 43.343826 3
TTTGC 5736970 1.0049093 14.721788 7
ACGTC 4401995 1.0010026 13.708052 6
GTAGT 7639315 0.9993885 9.982814 5
TATTT 11473630 0.99520767 65.24358 5
AGATC 7185545 0.993682 5.403283 145-146
ACGTA 7170405 0.9915883 8.677255 6
GATTA 10465280 0.99053293 36.143246 4
CACTC 3659900 0.99012715 15.34864 4
TGTTA 9230935 0.9833137 9.238471 9
GTTGT 6670750 0.9821613 8.247264 7
TCGAA 7040255 0.97358984 15.331588 2
TTCCG 3786655 0.9691023 26.50268 3
CGATT 6214985 0.9672887 59.433662 3
TATCT 7538280 0.955333 6.202234 7
ACTCT 5136255 0.9510398 19.635765 5
CATGA 6787075 0.93857783 16.630087 3
TGGCG 3539600 0.93511593 7.240669 9
CTCTG 3638025 0.93106407 13.724371 6
CCTCT 3041405 0.92602843 6.018507 7
CGACT 4041025 0.91891885 32.846 2
CCTGC 2454540 0.91781193 7.893251 7
GATGT 6997795 0.91546375 19.85253 3
GGTCT 4250035 0.9142611 8.603506 7
TGTCC 3530885 0.9036442 14.108974 9
ATGTC 5802925 0.9031564 15.498637 4
TTATT 10338735 0.8967684 5.530831 8
TCTGA 5745770 0.8942609 8.032656 7
ACCTA 5434980 0.8941735 7.9539795 6
TCTGC 3489330 0.89300925 29.825922 3
CATGC 3926835 0.89295226 5.015842 7
ACCAT 5414340 0.89077777 15.580086 5
TGTGA 6807650 0.89058846 15.486802 9
GTCAC 3894235 0.88553923 5.0857015 8
TTTGT 7381090 0.88490194 22.153025 7
AGACT 6391935 0.88393444 7.3519654 3
CTATT 6949800 0.8807543 29.284195 4
TGTCG 4078025 0.87725854 13.596522 3
TCGCA 3856955 0.87706184 5.426282 9
CGGTC 2783885 0.87498116 8.316804 6
GGACA 5134525 0.87201 23.110058 2
GTACT 5595835 0.8709253 6.5094886 5
CTGTA 5594645 0.87074 7.7339606 3
CTGAC 3809300 0.8662251 5.562382 1
CTAGC 3789360 0.8616908 5.591148 2
AGGAC 5072865 0.8615381 23.096432 1
TCACC 3182710 0.86103106 17.948921 3
CATCT 4618465 0.8551647 9.156763 5
GATCT 5489145 0.8543203 7.2342277 5
ACCGG 3051325 0.85213333 5.0928645 80-84
GTGGA 5294935 0.85069525 8.622928 8
GGCGT 3213180 0.84888005 73.40009 1
GTGTG 4676065 0.8455167 13.790766 8
TCCTA 4546730 0.8418821 7.2271433 3
TGAGT 6425615 0.84061015 31.981846 5
ATTAA 12271440 0.84033376 5.6518083 7
ACAGG 4938715 0.83875513 10.846076 1
TATTC 6617965 0.83870065 11.009113 6
TGTGG 4621995 0.83573985 17.901384 9
ACCTC 3067890 0.82996833 12.87019 6
CCATT 4475810 0.8287505 18.503185 5
GTTCC 3224750 0.8252964 21.398869 2
AGTCT 5300720 0.82499415 6.318235 5
CTAGG 4297880 0.8214928 11.67733 1
CCTAT 4363470 0.80794936 42.8477 3
ATGGA 6922120 0.8046198 6.2077627 4
TATGC 5168855 0.80447096 6.920009 7
ACTCG 3534285 0.8036874 7.499847 3
ATTCC 4334010 0.8024944 19.154465 2
GAATT 8452230 0.7999989 13.420577 2
AGCGC 2857095 0.79789144 10.080804 4
TCTGG 3671375 0.78978074 9.329368 7
ATATT 10242445 0.78938377 28.636646 5
CCGGT 2505690 0.7875439 31.920424 5
CACAC 3275105 0.78726083 9.602419 3
GGATG 4898185 0.78695256 11.6721325 6
ATGTT 7386520 0.78683966 9.26034 8
GGATA 6745240 0.7840594 11.380365 1
CTAAT 6952820 0.78291607 37.50555 4
AACGA 6351650 0.78045166 5.813005 2
CGATA 5639795 0.77992177 52.78245 3
GACAT 5582815 0.77204204 16.593702 1
TAGAA 9179965 0.77202404 6.106209 9
GTAGG 4801295 0.771386 6.20514 3
GCAAG 4539705 0.7709902 7.2947993 3
CTACT 4151240 0.7686523 6.1203914 1
CCTGG 2442780 0.7677711 6.064426 7
GTGCA 4004035 0.7653276 8.785148 8
GTGTC 3553610 0.76444733 16.262524 8
GATCC 3335915 0.75857866 8.874828 1
TGTTT 6327105 0.7585421 20.304737 9
ATAAC 7502200 0.7506103 9.831316 3
ATTGT 7031630 0.7490355 15.650886 7
GACAC 3684625 0.7444759 11.465673 2
GCATC 3259105 0.74111223 8.461266 2
ACGCC 2224095 0.738939 8.204335 2
TCTCT 3501035 0.72958666 9.964643 7
CTGGA 3797415 0.7258344 6.0302987 8
GTCAT 4654245 0.724378 6.916184 3
GCGCT 2295565 0.7215011 10.901336 5
GGATT 5485350 0.7176029 6.8549275 5
ATTTC 5654700 0.7166252 21.374231 6
GGAAT 6026155 0.7004737 16.458275 1
TAATT 9078185 0.6996544 25.008284 5
AGTGG 4348485 0.6986366 7.1487265 7
GTGTA 5317835 0.6956883 6.982494 8
CGCCA 2092960 0.69537044 8.20962 3
ATTAC 6158045 0.6934211 14.616471 3
TACTC 3743350 0.69312656 6.099244 2
CCTGT 2664800 0.6819909 12.716892 2
GGACT 3526910 0.67413026 9.979849 4
AGTGA 5765890 0.67022085 6.6653504 7
ATGAG 5709800 0.663701 23.339144 4
GCCAT 2849135 0.6478861 5.6208997 4
TACGT 4154270 0.64656276 30.35702 5
CGTCT 2512925 0.6431222 8.7528305 7
CACAT 3906445 0.64269596 6.4889574 5
GGATC 3351505 0.6406036 6.2905993 6
TATTA 8276680 0.63788253 6.1682143 6
CTGAG 3326750 0.6358719 6.896309 1
ACGAT 4548095 0.6289516 6.5455766 3
TGAGC 3273785 0.6257483 5.5229616 9
GAGTG 3881380 0.6235905 21.682741 6
TCGAT 3988965 0.620835 5.0816097 5
GTCTC 2423940 0.6203485 10.687872 6
TAGCA 4484480 0.6201544 5.93931 1
ACGAC 3053905 0.6170394 14.348558 1
CTGTC 2398025 0.61371624 11.345712 8
TGTGT 4086660 0.60169536 19.026752 5
ATCTG 3848615 0.5989912 7.257739 6
CTAGT 3847550 0.59882545 12.872609 5
TTGTG 4064055 0.59836715 14.723045 8
AGTGT 4573065 0.59825635 11.156823 7
TACGA 4293285 0.5937141 15.539107 1
TTGTA 5560825 0.59235984 7.351045 8
CGTAG 3079310 0.5885765 7.5646086 2
GTCTG 2730465 0.58737355 20.770544 6
TCTGT 3335635 0.5842824 18.723873 7
AGTGC 3047615 0.58251834 8.770917 7
TGGGA 3614875 0.5807733 5.006292 9
TGTGC 2667660 0.5738631 8.550948 7
GTCGA 2900570 0.5544123 6.647477 4
AGACA 4501925 0.55316883 6.977418 1
ATTTA 7168185 0.552451 11.610534 6
GCGTA 2830300 0.540981 53.1946 2
GCATG 2820215 0.5390533 10.051143 2
CATGT 3459140 0.53837407 12.702281 5
GAGTA 4533835 0.52700806 8.252706 6
TGTAA 5557940 0.52605593 9.195017 9
TAGGT 4007615 0.5242832 7.21427 1
TCCGG 1655230 0.5202424 32.551785 4
CGATC 2275020 0.5173338 10.765447 4
TCTCG 2020765 0.5171657 5.701345 9
AGCGG 2195895 0.5154595 6.5592775 4
CAGAG 3028380 0.51431787 5.423529 4
TAGTG 3695345 0.4834314 10.30868 6
TAGCG 2467580 0.47165096 6.9011455 3
TATGT 4377085 0.46626344 10.930471 7
TAGTC 2960195 0.46071923 5.136737 6
TTGCG 2129790 0.4581572 5.347861 8
GAGAC 2691850 0.45716402 9.031399 2
CTCTC 1500620 0.45689958 6.8808084 8
TTCGC 1771560 0.45338774 11.4075 1
TGAGA 3850510 0.44757915 8.319421 1
TCGCC 1193230 0.44617754 16.694132 2
TCGCG 1372685 0.43143788 7.148129 2
ATGTG 3260365 0.42652655 6.8232484 4
GTGAG 2624685 0.42168733 8.031342 4
GTCTA 2674345 0.41623005 6.6257033 6
TCTAG 2540825 0.39544925 7.157051 4
AGCAT 2769960 0.38305506 12.393022 1
TTATA 4964655 0.38262525 5.725825 6
CGCGT 1215840 0.3821412 7.149039 3
GAGTC 1808035 0.34558615 11.190579 6
GTACG 1807275 0.34544086 13.197685 4
TGTAC 2136960 0.33259243 5.261501 9
TGCGA 1688290 0.3226982 5.404555 9