FastQCFastQC Report
Tue 9 Jul 2019
S_1_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename S_1_R2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 46874872
Filtered Sequences 0
Sequence length 100-150
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGGTG 19898415 3.4371362 23.219717 6
TTGGT 22998635 3.1025207 24.227243 5
TCTTG 19201450 2.9965959 28.364965 9
TGAAG 25238550 2.8804927 8.893821 9
TGCTG 14191430 2.8358676 11.212409 9
CTTGG 14085245 2.8146489 13.914164 3
TGATG 22185340 2.752793 31.106302 2
CGTCG 9075980 2.686585 10.601198 145-146
GTCGT 12787920 2.555405 7.817798 145-146
TCAAG 19257410 2.5426223 6.4073124 9
CTGGT 12661300 2.5301025 19.369902 4
TCGTG 12564900 2.510839 9.28397 6
TTGCT 15723275 2.453789 21.465754 4
TTGAA 27363125 2.4389474 10.891839 8
TGGCT 11793425 2.3566754 9.370778 9
GCCGA 8255400 2.2477088 223.79996 1
TTGAC 15519025 2.2276838 5.4777975 8
GGTGA 13728530 2.1812084 7.1926575 7
AAGGT 18844230 2.1507046 8.595022 1
ACTTG 14969940 2.1488652 12.530539 4
TGAAA 26143605 2.14337 14.025201 9
TGGTA 17163580 2.129685 10.04164 6
CTCTT 11719760 2.1158967 13.777588 8
CCTTG 8996790 2.0798354 7.4239078 6
CTTCA 12243710 2.0332167 5.858654 7
TTCAA 19709760 2.0323558 10.144908 8
GGTGG 9188190 2.0322347 7.9452124 7
TCTTC 11147875 2.012648 14.660174 9
CTGCT 8597415 1.9875097 26.428642 4
TGGTT 14606635 1.9704381 12.314724 5
GCTCT 8510160 1.9673386 11.145486 2
CTCAA 12875715 1.9666942 7.3215823 8
CTTCT 10784285 1.9470052 15.903401 5
CAACT 12741150 1.9461402 14.76802 1
CTGAA 14665730 1.9363669 7.5539675 8
TGCTA 13468750 1.9333763 9.4534025 9
ATGCT 13466495 1.9330527 5.0127144 15-19
CTTGC 8337965 1.9275315 5.887241 7
ACCTT 11535755 1.9156523 11.591065 5
ACAAC 13553855 1.9042469 9.624602 4
TGGTC 9481110 1.8946064 15.739736 6
TTTGG 14024220 1.89187 9.5324955 7
GTGGT 10906370 1.8839028 5.764753 8
TTGGC 9411980 1.8807921 9.734938 8
GTTGC 9366605 1.8717248 27.487461 3
TGTTG 13872275 1.8713728 21.120262 9
GATGA 16277185 1.8577261 9.0314865 3
GTCTT 11895820 1.8564727 18.24224 8
ATGGT 14896990 1.8484428 16.50848 5
TTGCC 7864995 1.8181925 9.132806 8
GTCAA 13693530 1.808004 9.265108 8
TGCAG 9709590 1.7846606 7.1763163 9
ATCGG 9694415 1.7818714 8.544537 145-146
TGCCA 8356750 1.7769439 11.220473 9
TCAAA 18685070 1.7721806 9.733307 9
TTTGA 18203580 1.7640002 7.804684 7
TGCTC 7570710 1.750161 5.858118 9
GGTGC 6801585 1.7403461 8.246706 7
CCAAG 8828060 1.7266189 11.192916 1
TTATC 15369325 1.722972 14.694547 6
GTTGG 9970690 1.7222788 10.539512 2
TTGGA 13873050 1.7213907 9.516992 8
AACGT 13014495 1.7183486 13.383888 5
TTCTT 13943865 1.6994629 20.938148 8
ACTGG 9220455 1.6947557 17.819822 3
AGCTC 7933505 1.6869469 10.215938 1
CGTGA 9099390 1.6725032 7.3004766 7
TTCCA 10060725 1.6707056 14.28594 3
GCCTA 7808890 1.6604493 120.54923 2
TGACT 11564140 1.6599784 14.180935 5
TCGTC 7110110 1.6436818 7.283649 1
TCAAC 10661050 1.6284168 9.060493 9
GAAGG 11100930 1.622286 9.412111 3
GCGTC 5458450 1.6157583 9.858409 145-146
CTCGT 6945500 1.6056279 6.9535847 4
ACTTC 9589320 1.5924232 14.766148 4
GGTAA 13950680 1.5922005 5.774278 7
CAAGG 9332320 1.5777538 9.682092 2
CGAAG 9312285 1.5743666 10.875538 2
TCATC 9447620 1.5688922 5.237297 4
AATTG 17595655 1.5683471 12.833911 3
TATCG 10845335 1.556797 7.0493107 9
CCGAT 7276655 1.5472771 175.48203 2
GGTGT 8925405 1.5417224 16.901312 7
TGCTT 9832415 1.5344558 38.736275 5
GTGAA 13416945 1.531285 11.008388 8
CGTGT 7587980 1.516303 16.88372 7
CATGG 8206785 1.5084392 24.76565 4
TCTCA 9044390 1.5019308 20.807192 9
ATTGA 16829565 1.5000635 7.559521 7
ATTGC 10425745 1.4965671 7.8250394 7
GAGCG 6345430 1.4934182 7.4545546 140-144
AGCTA 11294380 1.491236 13.267455 1
GGAAG 10159315 1.4846787 5.1346526 135-139
GCTTC 6421280 1.4844413 17.004108 6
GGTAT 11945465 1.4822128 36.803417 3
TTCGT 9483475 1.4799999 15.551253 5
ATTGG 11889400 1.4752562 17.856936 4
CGTTG 7368355 1.4724154 12.187841 1
GTGAC 7993780 1.4692879 18.34042 4
TTGTC 9402925 1.4674292 17.48735 8
TTCTC 8116645 1.4653869 16.148884 8
CTGAT 10158225 1.4581659 8.802701 1
AACTG 11040230 1.4576795 12.76023 2
AACCT 9512265 1.4529458 36.72226 5
GGTAC 7848705 1.4426225 13.992258 3
GGTTG 8250125 1.4250785 23.782122 2
CTGGC 4769580 1.4118456 6.5122223 8
CTTCG 6098895 1.4099138 6.6703877 1
CGGTA 7660785 1.4080821 54.366653 2
ATCTT 12551090 1.407035 9.079916 8
GTGCT 7026330 1.4040687 10.575239 8
TCAGC 6577135 1.398534 10.529384 9
GCTTG 6995340 1.3978759 18.996338 6
ACGTG 7589600 1.3949982 25.779032 6
TATTG 14292405 1.3849916 15.178866 6
TCGTT 8860130 1.3827201 11.902331 2
CGTCA 6466315 1.3749698 15.791765 2
CGTGG 5334385 1.3649281 8.346373 7
GTGAT 10991250 1.3638122 23.468975 1
TGAAC 10327390 1.3635609 6.105043 9
GTGTT 10084515 1.3604032 21.488283 8
GCTGT 6802930 1.3594267 6.0263553 4
GACTT 9454165 1.3571013 25.296892 3
TCGGA 7341970 1.3494827 6.1741076 135-139
GTTCA 9387725 1.3475642 10.423478 1
GGTTT 9954300 1.3428372 7.5771422 5
GACTG 7304115 1.3425249 7.2894316 6
CGGTG 5216835 1.3348502 14.982826 6
CTGTT 8553290 1.3348343 14.51784 8
GTATT 13768550 1.334228 58.396393 4
TCTTT 10868440 1.3246335 13.247297 9
TTTCA 11795505 1.3223304 9.065955 7
AGGTT 10597015 1.314895 17.134916 1
ATTTG 13538275 1.3119134 36.42806 6
GCGGT 5126635 1.3117706 75.351715 1
TGGAA 11492550 1.3116525 13.300035 9
GAACC 6678455 1.3061926 47.349873 4
TTCAG 9087730 1.3045013 7.8682737 8
GGTTC 6510270 1.3009446 12.496589 3
TGGAT 10424450 1.2934828 15.800203 5
GATCG 7019690 1.2902465 7.924725 145-146
CATCG 6051000 1.286659 6.549728 1
TGACA 9696350 1.2802426 5.1580844 9
GTTCT 8186915 1.2776575 17.766663 1
CGTGC 4301155 1.2731868 8.873133 7
CGTTC 5502740 1.2720975 19.892996 1
CTTGT 8139930 1.2703251 13.53516 5
TTGTT 12030885 1.26749 21.374973 8
AGCAA 10432160 1.2669314 5.2408648 2
ATCCA 8274400 1.263869 20.534163 1
TTGCA 8800800 1.2633139 6.666748 8
AGAGC 7447595 1.2591158 5.736078 140-144
AGGTA 10984715 1.253693 8.687406 2
GGTCA 6820330 1.2536032 7.9195514 7
GTAAA 15272900 1.2521408 7.0664682 8
CGGAA 7396830 1.2505332 5.5786333 135-139
ATCGT 8696310 1.2483147 5.425937 1
TGCCG 4206135 1.24506 6.7027497 9
CCATG 5754505 1.2236135 28.641203 3
GTGCC 4133245 1.2234837 8.602666 8
ATCGA 9251115 1.2214566 15.589731 1
GCTAC 5730755 1.2185634 21.435795 2
ACGAA 10022605 1.2171931 14.968259 2
TGTCT 7796935 1.2167969 27.473923 5
GATAT 13635775 1.2153926 34.171665 4
TTCAC 7311950 1.2142383 12.08558 2
ACACA 8633095 1.2129055 5.765627 4
TATCA 11760280 1.2126514 5.411169 9
CACCT 4909685 1.2077346 17.908953 4
TCACA 7906435 1.2076645 8.884435 9
GGCCT 4024665 1.191343 91.08688 1
CATTG 8289875 1.1899729 6.6214347 6
TCAGG 6453925 1.1862566 10.798142 1
TGCAA 8964850 1.1836601 7.241852 9
TGTTC 7578015 1.1826321 16.560036 9
CACAG 6044340 1.1821705 6.519796 2
TTATG 12194160 1.1816633 18.98772 6
CAACG 6014735 1.17638 15.774416 1
ATCCT 7079605 1.1756544 6.6530256 2
TTTCT 9623455 1.1728961 16.628744 7
CTCAG 5504910 1.1705406 5.6170864 8
CCATC 4746460 1.1675829 7.8590655 6
GTATC 8125295 1.1663482 5.7271237 8
ACATG 8809030 1.1630865 15.922302 2
TGGCA 6266045 1.1517235 8.871185 9
CGCCT 3356710 1.1494827 88.08325 1
TGTCA 8002610 1.1487374 19.643766 9
GTGGC 4469415 1.1436054 9.459952 8
CAGGA 6747185 1.140702 22.472652 2
TGTAG 9173910 1.1383138 6.473847 4
CGTAT 7857550 1.1279147 43.932026 3
ACGTT 7836985 1.1249627 6.274623 3
GACAA 9243285 1.1225488 8.321689 3
TTTGC 7176000 1.1198931 10.599546 7
CGAAC 5693165 1.1134864 47.3401 3
TACTG 7755910 1.1133248 6.093994 4
CGATT 7748290 1.1122308 64.25996 3
TTCTG 7122765 1.1115853 17.814005 2
AGCGT 6039600 1.110102 6.331356 145-146
TCCAC 4485465 1.1033806 6.3849225 1
GATAA 13447940 1.1025224 8.346823 2
ACACT 7187900 1.097912 9.667187 3
GATGT 8831430 1.0958183 21.316206 3
CCACA 4827400 1.0922601 5.8853226 2
TCCAT 6568320 1.0907493 22.355053 2
TATCT 9700355 1.0874544 9.160351 7
CCTAA 7081435 1.08165 47.36905 3
GTAGC 5869870 1.078905 5.704217 8
ATCGC 5039420 1.0715609 5.471757 1
TATTT 14135495 1.0697622 64.16509 5
ATTCA 10355915 1.0678414 9.189528 7
ACGTC 5005560 1.0643611 17.38255 6
TCGAA 8059905 1.064177 15.643742 2
AATTC 10292060 1.0612571 10.869372 1
AGATC 8032570 1.060568 6.0012603 145-146
TAAAA 17990340 1.0594983 7.6464534 9
ACTGT 7352835 1.0554652 16.5658 1
ATCTC 6287185 1.0440636 6.901786 8
ATGTC 7242980 1.039696 15.902003 4
CCTCT 3885760 1.0391996 7.946169 7
GGACA 6129335 1.0362463 24.109694 2
ACCTG 4866770 1.0348493 21.675035 6
CACTC 4203930 1.0341258 15.584377 4
GTTCG 5163750 1.0318701 20.11182 4
CATGA 7770860 1.0260135 15.928804 3
TCGAC 4822805 1.0255008 18.812918 1
CTACC 4162685 1.0239799 24.79986 3
GACTC 4802160 1.0211109 13.171351 4
GATTA 11433865 1.0191306 39.907467 4
ACCAT 6658540 1.0170554 17.610838 5
CTGCC 2968120 1.0164126 5.894894 8
CTATT 9028030 1.0120838 28.854782 4
CACAC 4465085 1.0102818 9.285855 3
ATTAT 14444665 1.005492 31.023905 5
ATTCT 8921225 1.0001104 12.278895 7
ACTCT 6020675 0.9998062 20.49952 5
CCATT 6013340 0.99858814 17.987516 5
GTTCC 4303355 0.99482936 19.889147 2
CCTAT 5958770 0.98952603 42.804314 3
TCTGA 6860475 0.9847892 7.220483 7
ACGTA 7442675 0.9826821 9.783708 6
ATGGA 8599260 0.98143935 6.717611 4
GTTGT 7272710 0.9810901 8.133763 7
TCTCT 5388140 0.9727799 14.369073 7
GTCAG 5273805 0.969346 7.1324005 8
GCTTA 6745905 0.96834326 9.065434 6
GTGGA 6092555 0.96799386 9.088513 8
ACCTC 3927380 0.9660972 15.938976 6
CTGAC 4520865 0.9612976 6.309255 1
TACCA 6270760 0.9578241 15.401479 4
TCTTA 8542115 0.95761037 8.33441 9
ATTCC 5760665 0.9566284 17.500458 2
AACGC 4890705 0.95653886 5.105016 1
TCACC 3886075 0.95593655 17.908062 3
CTCTG 4130690 0.9549134 12.501891 6
TCTAA 9236655 0.95243007 5.598483 9
GGCGT 3715540 0.95070857 77.811 1
TGTTA 9782345 0.9479487 9.772677 9
CATCT 5691380 0.9451228 9.495545 5
TCAGA 7156735 0.9449284 5.9141407 9
CATTC 5671295 0.94178754 5.084723 6
GAATT 10548595 0.9402241 12.816165 2
GGATG 5905185 0.9382242 11.206612 6
AGGAC 5536355 0.93599504 24.015198 1
GGTCT 4669645 0.93313324 11.778398 7
TAACG 7019300 0.92678237 13.4661 4
TATTC 8242255 0.9239947 12.395774 6
TTAAA 14413985 0.92289025 6.3417664 8
TTACG 6400100 0.9187044 19.755697 4
TTTGT 8687160 0.9152184 20.76483 7
TGGCG 3576645 0.91516894 5.5073133 9
AGTCA 6892220 0.9100036 6.4723167 7
CTCTA 5443755 0.90400153 8.005873 3
TCTGC 3906610 0.9031117 26.42848 3
ATGTT 9282265 0.8994889 9.608432 8
TTCCG 3884165 0.89792293 24.377636 3
TAAAC 9452260 0.896497 5.1470294 9
GACAT 6777090 0.8948026 15.887317 1
GTCAC 4181510 0.8891386 6.8256097 8
GTACT 6171510 0.8858915 6.8764772 5
TGTTT 8403140 0.88529617 20.105185 9
CGACT 4131720 0.8785513 37.442932 2
TCTGG 4389675 0.87718695 8.469711 7
ATTTC 7822965 0.8769904 27.092106 6
CTGTG 4388040 0.87686026 19.875702 2
TGAGT 7050180 0.8747978 30.899946 5
ACAGG 5145005 0.86983204 12.533388 1
GCCAT 4081750 0.867926 5.5787535 4
CCTCA 3521775 0.8663222 5.643488 7
GACAC 4428610 0.86616105 12.377728 2
TGTCG 4332390 0.86573976 12.299535 3
TTATT 11438345 0.86564416 6.5902796 8
CCTAG 4066485 0.86468 8.524005 4
AGTCT 5939385 0.85257107 7.819739 7
TGTCC 3655985 0.84517336 11.137286 9
GTGTC 4204180 0.8401196 17.06645 8
GGTTA 6759560 0.83873725 5.4818645 6
AGACT 6326840 0.8353545 8.172273 3
CGGTC 2817300 0.8339503 10.163747 6
CTCAC 3362965 0.8272565 5.0504117 8
GGATA 7229810 0.8251431 11.593509 1
ACCTA 5398120 0.8245331 9.008516 6
CGTCT 3563920 0.82389015 12.119272 7
GTGCA 4479910 0.8234249 6.5617657 8
ACTCG 3870700 0.82304925 6.392543 3
GAGGA 5615540 0.820653 5.662291 4
TCTCC 3056180 0.8173384 5.814114 9
CCTGG 2750875 0.8142878 5.3261943 7
GTGTG 4711240 0.8137921 12.857919 6
TATGC 5664150 0.8130623 5.3295946 7
GCAAG 4780680 0.808238 7.3041353 3
CCTGC 2350580 0.8049403 5.512827 7
ATATT 11522925 0.8021099 26.36434 5
GTGTA 6432795 0.79819167 7.089501 8
ATGAG 6939095 0.7919636 22.799305 4
ATTGT 8130510 0.78787917 13.144153 7
CTGTA 5482585 0.7869995 7.4843593 3
GCATC 3698250 0.7863802 5.2132716 2
TCCTA 4705345 0.78137964 6.6538663 3
TGTGG 4521110 0.7809502 11.060772 9
CTACT 4674335 0.7762301 5.014988 1
TACGA 5841305 0.7712477 16.227346 1
GATCT 5367845 0.77052915 7.2887645 5
ATTAC 7465970 0.7698473 14.190686 3
AACGA 6338430 0.76976925 6.1906624 2
TCGCA 3582895 0.7618516 5.1207466 9
TGGAG 4765635 0.7571709 5.0930777 1
CTAGC 3554795 0.75587654 6.3871546 2
GGATT 5993150 0.7436398 6.1949024 5
CTGGA 4033615 0.7413941 6.375857 8
TGTGA 5959385 0.7394502 12.941764 9
GTCTC 3179145 0.73493975 13.8323555 6
GGAAT 6429675 0.7338232 16.38603 1
GTCTG 3657525 0.73088175 18.826628 6
GTAGT 5886895 0.7304555 10.319956 5
CGATA 5523695 0.7293125 50.627285 3
GATCC 3421425 0.7275173 8.516462 1
CTGTC 3129205 0.7233949 11.711047 8
CTAAT 6955265 0.71718633 31.984514 4
ATTAA 11141730 0.7133762 5.5900426 7
CCTGT 3049180 0.704895 12.050816 2
GAGTG 4427775 0.7034913 18.948322 6
AGTGG 4416070 0.7016315 6.3614297 7
CTGAG 3790625 0.6967317 5.3391867 1
GTCAT 4848520 0.69598246 7.5457816 3
TCGAT 4847420 0.69582456 5.474992 5
CGCCA 2198220 0.69239616 8.257274 3
GGACT 3757905 0.69071764 10.836336 4
ACGCC 2187085 0.68888885 8.275062 2
AGACA 5591745 0.6790883 7.675065 1
CCGGT 2290230 0.677932 30.916018 5
GCATG 3672615 0.6750409 10.775386 2
GCGTA 3667285 0.67406124 56.131653 2
AGCGC 2461000 0.67005974 10.135782 4
ACGAC 3423205 0.66952085 17.151247 1
TCTGT 4272700 0.6668015 18.338736 7
CATGT 4625790 0.6640106 13.489909 5
ATCTG 4625555 0.66397685 6.3940377 6
GAGAT 5804825 0.6625086 5.0501695 5
TAGAA 8080115 0.66244406 7.3285937 9
AGAGT 5746180 0.6558154 5.2362576 5
AGTGA 5730155 0.65398645 6.143563 7
AGAGG 4449820 0.65029514 5.663138 3
TGAGA 5687495 0.64911765 9.202619 1
ACGAT 4913935 0.64880383 6.751261 3
CAGAG 3837060 0.64870644 5.6445723 4
TTTAT 8571025 0.6486478 5.533925 7
TCTAT 5776430 0.6475644 5.33461 7
TTAGC 4478650 0.6428893 5.409724 8
CTCTC 2390025 0.6391832 10.045499 8
TAGCA 4720620 0.62327975 6.826112 9
GTCGA 3380045 0.6212654 7.1760497 4
AGTAA 7570955 0.62070084 5.3798065 7
TTGTA 6386115 0.61884034 7.047797 8
AAGCG 3648855 0.61688787 5.005497 3
TATTA 8859970 0.6167418 6.2286296 6
TACGT 4170640 0.59867597 30.341618 5
ATAAC 6257130 0.5934558 9.67535 3
TAAGC 4474440 0.5907757 5.0575185 9
AGTGT 4746440 0.58894604 10.338014 7
GGATC 3203825 0.5888755 8.6461525 6
TTCGC 2537710 0.58665586 12.962007 1
CTAGT 3995460 0.57352966 12.514222 5
TTGTG 4245310 0.5726932 10.670742 8
TGTGT 4236705 0.57153237 20.100489 5
CACAT 3721760 0.56847835 6.2397957 5
CTAGG 3090360 0.5680203 11.958176 1
ATTTA 8068430 0.56164277 12.7906475 6
GCGCT 1875075 0.5550418 10.789552 5
GAGAC 3265265 0.5520368 10.469277 2
TCTCG 2373905 0.5487882 8.182619 9
TGTGC 2739090 0.54735124 6.675679 7
AGCGG 2289635 0.5388732 6.962534 4
AGTGC 2875955 0.5286117 6.1794353 7
TAGGT 4216545 0.5231958 7.8145766 1
TATGT 5331240 0.51661867 10.951367 7
CGTAG 2807565 0.51604146 5.3160315 2
TGTAA 5784330 0.5155726 9.641179 9
GTCTA 3581135 0.51405525 7.6187396 6
GTGAG 3222815 0.51204544 8.328376 4
TAATT 7323005 0.50975376 21.32507 5
TCGCC 1479720 0.5067201 19.334688 2
AGCAT 3667125 0.48418316 10.883808 1
TAGTC 3354525 0.48152643 6.3073606 6
CGATC 2263695 0.4813425 10.880548 4
CTAGA 3536580 0.46694687 5.110096 1
TAGTG 3760940 0.46666357 9.137789 6
TCTAG 3201370 0.45954174 6.9231415 4
ATGTG 3693490 0.45829427 7.581719 4
GAGTC 2486220 0.45697695 13.18914 6
TCCGG 1445035 0.42774546 31.216703 4
TTATA 5820580 0.4051701 6.5497704 6
GGAGT 2532165 0.40231398 5.1012454 2
CGCGT 1293420 0.38286585 7.5280824 3
GAGTA 3353130 0.38269502 9.75915 6
TAGCG 2034435 0.3739371 6.844555 3
GTACG 1905615 0.35025948 13.873288 4
TCGCG 1095480 0.32427353 7.515652 2