FastQCFastQC Report
Tue 9 Jul 2019
S_3_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename S_3_R2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 47613239
Filtered Sequences 0
Sequence length 100-150
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGGTG 17772105 3.0504994 24.418268 6
TGGCT 14973835 3.0045545 11.1329775 9
TTGGT 21416335 2.853223 24.565825 5
TCAAG 21578365 2.7970436 5.899263 9
TCTTG 17706140 2.7575884 22.474743 9
TGCTG 13286770 2.6660388 11.426531 9
TGATG 21494015 2.6124365 31.303741 2
CTTGG 12226605 2.4533129 14.490408 3
TGAAG 21997615 2.4391663 7.7150335 9
CTGGT 12071575 2.4222054 19.799202 4
GCCGA 8362825 2.305654 232.44057 1
TTGCT 14752695 2.2976134 20.664015 4
CGTCG 7471390 2.2579012 8.4657 145-146
CTGGC 7427670 2.2446885 7.403501 8
ACTGG 12119800 2.2186055 18.065327 3
TCTTC 12176960 2.2169678 15.148259 9
ACTTG 15226455 2.1634257 11.584377 4
TGCCA 10054695 2.1516347 11.9176855 9
TGCTA 15088885 2.1438792 10.763705 9
GCTCT 9127455 2.1409757 11.62076 2
TTGAA 24815160 2.1357055 10.667034 8
GTCTT 13486635 2.1004345 16.337257 8
GTCGT 10407450 2.0882928 7.098717 4
TTCAA 20621150 2.074685 9.385763 8
GCCTA 9638335 2.0625365 128.78105 2
CTGCT 8784135 2.0604448 29.661007 4
GTTGC 10182775 2.0432107 26.629625 3
TCGTG 10134310 2.0334861 9.294563 6
GGTGG 9086670 2.0094552 9.374682 7
CAACT 13222325 2.0035655 14.950145 1
TGAAA 25385440 1.9931804 14.187393 9
TGGTA 16320800 1.9836707 9.784496 6
AAGGT 17886845 1.983351 8.380765 1
CTCAA 13059270 1.9788578 6.659212 8
CTGAA 15165120 1.9657422 7.204489 8
GGTGA 12544175 1.9643178 7.5440674 7
CTTCT 10755815 1.9582305 14.898371 5
ATGCT 13776290 1.957381 6.0140343 15-19
TGGTT 14656195 1.9525933 12.231245 5
TTGAC 13225620 1.8791401 5.55564 8
CTCTT 10243080 1.8648807 11.992833 8
TGCAG 10151225 1.8582456 8.793462 9
TTGGC 9192040 1.8444164 11.52235 8
TTTGG 13672425 1.8215292 10.602957 7
CTTCA 10878030 1.8067926 5.3519163 7
CTTCC 6571430 1.8019254 5.6641746 25-29
TGGTC 8870650 1.7799282 15.376296 6
CTTGC 7578945 1.7777503 6.9659724 7
AGCTC 8303840 1.7769637 10.613693 1
GGCTG 6869640 1.7759166 5.294149 30-34
ACAAC 12723710 1.7589239 9.704074 4
GTGGT 10217245 1.7537427 5.670083 8
CATGG 9512725 1.7413641 25.369019 4
TGTTG 13058705 1.7397653 19.524609 9
TCAAA 18836595 1.7289371 9.5825615 9
ATGGT 14212885 1.7274696 16.643835 5
CCTTG 7355400 1.7253146 7.3125315 6
ACCTT 10305290 1.7116632 11.012205 5
GTCAA 13025260 1.6883681 8.732835 8
TTGCC 7112260 1.6682827 9.589266 8
GGTTT 12323385 1.6418012 7.133351 5
GATGA 14745875 1.6350698 9.549043 3
TGCTT 10496675 1.6347723 39.66604 5
GAGCG 6920860 1.6322483 5.8697047 140-144
CTTCG 6948040 1.6297624 6.481944 1
CCGAT 7605400 1.6275024 180.68208 2
GTGCT 8053075 1.6158788 11.270317 8
CCAAG 8269975 1.6145124 10.708931 1
TTCCA 9713260 1.6133296 15.134722 3
AACTG 12426780 1.6107916 12.785818 2
GAAGG 11234020 1.6048796 9.387154 3
AGCTA 12350860 1.6009505 12.565136 1
AACGT 12316580 1.5965072 13.775828 5
GCTGT 7954895 1.5961787 6.143288 4
TTATC 14455045 1.5941204 13.070463 6
TGCTC 6771345 1.5883161 5.773212 9
GCTTC 6756020 1.5847214 15.514033 6
CCATG 7355150 1.5739508 29.647585 3
TGACT 11072115 1.573163 13.759877 5
GTTGG 9097195 1.5614914 11.604445 2
GCGGT 6032560 1.5595175 75.149414 1
TTTGA 16432475 1.550207 7.735549 7
TCAGC 7204545 1.5417222 10.654259 9
TTGGA 12663600 1.5391657 12.033217 8
TCGTC 6553670 1.5372571 7.306761 1
TTTCA 13915765 1.5346478 8.474584 7
CTCGT 6528660 1.5313909 7.2600894 4
TCAAC 10083455 1.5279355 7.5514336 9
TCATC 9193585 1.5270138 5.1563897 4
TTCTT 12602095 1.5233752 18.37061 8
ACTTC 9118825 1.5145968 13.74741 4
GGTAA 13611760 1.5093157 5.755789 7
CGAAC 7667895 1.496971 46.657745 3
TCTCA 8976040 1.4908805 19.443989 9
ATCGG 8131940 1.4886025 7.615513 145-146
TATCG 10428635 1.4817353 9.421314 9
CGTGA 8088075 1.4805729 7.703153 7
GCTAG 8072900 1.4777951 5.146389 45-49
GTTCA 10398445 1.4774456 9.903866 1
CGGTA 8053435 1.474232 53.29242 2
GACTT 10357500 1.471628 23.34968 3
CTGAT 10323535 1.4668021 8.649369 1
GGTGT 8531080 1.4643203 16.60402 7
CGAAG 8701465 1.4531641 10.969246 2
GTGAC 7882840 1.4430034 17.92758 4
AACCT 9516235 1.4419854 35.960556 5
TCGGA 7863120 1.4393935 6.6682696 145-146
TCCAT 8646675 1.4361745 23.001198 2
GTGTT 10771330 1.4350264 20.94035 8
CTGCA 6693350 1.43233 5.1980004 8
GGTTG 8337125 1.4310288 22.80465 2
GTGAT 11750010 1.4281257 23.879913 1
GGTAC 7737415 1.4163824 13.953489 3
AATTG 16439850 1.4148884 12.812697 3
TTGCA 9910560 1.4081255 8.003569 8
ATTGG 11569745 1.4062159 18.08597 4
GTGAA 12579390 1.3948432 10.769469 8
TTGTC 8922405 1.3895926 17.909323 8
AGCAA 11731615 1.3873183 5.4158645 2
GTGCC 4589640 1.387018 9.949652 145-146
TGTCT 8887850 1.3842108 27.273535 5
GGTGC 5340620 1.3806392 9.18208 7
TTCTC 7517365 1.3686304 14.750836 8
AGGTT 11255525 1.3680246 16.23632 1
GCTTG 6783925 1.3612193 22.163458 6
CGTTG 6717675 1.3479259 13.558933 1
CATCG 6224130 1.3319204 6.4363422 1
GGTAT 10946355 1.3304476 35.391556 3
CGTCA 6211865 1.3292959 16.205278 2
ATTGA 15410515 1.326299 8.065614 7
ATTGC 9291635 1.3201865 9.216121 7
CTTGT 8456105 1.31697 12.591954 5
CAAGG 7883440 1.3165519 9.16752 2
ATTTG 13948060 1.3158323 36.28677 6
GTTCT 8395265 1.3074948 19.63475 1
CTCTG 5570335 1.306602 12.170299 6
CACAG 6690540 1.306166 6.4473376 2
TTCAC 7843680 1.3028008 11.595058 2
CATGA 10016325 1.2983422 16.67449 3
TTCAG 9129170 1.297103 7.314382 8
GTAAA 16519590 1.2970634 7.109149 8
TGAAC 9916540 1.2854079 5.612653 9
GACTG 7008620 1.2829719 7.402894 6
CCTAG 5992875 1.2824333 9.011653 4
TCTGA 8997530 1.2783991 7.242008 7
TGCAA 9838960 1.2753516 7.8216996 9
CGTGT 6351175 1.2743864 16.873682 7
TTGTT 12321115 1.2740871 21.078447 8
TGGAA 11485350 1.2735325 17.794382 9
ATCTT 11524500 1.2709361 7.6396365 8
GCGTC 4202310 1.2699646 8.082001 145-146
CGGCG 3249510 1.2652109 6.1411285 95-99
GAACC 6478285 1.2647284 46.65887 4
GTATT 13340680 1.2585332 56.55366 4
TTCGT 8062525 1.2556733 15.25402 5
GGTTC 6248125 1.2537091 12.328243 3
GTAGC 6836880 1.2515339 5.479888 8
ATCGC 5811955 1.2437177 5.5054955 1
GGCCT 4101415 1.2394735 103.410904 1
ACACA 8944095 1.2364304 5.8602524 2
CTAGC 5770905 1.2349333 5.953113 2
ACATG 9510265 1.2327453 16.682365 2
TCGTT 7903490 1.2309047 11.788061 2
TATTG 13026155 1.2288616 16.408535 6
ACGTG 6699285 1.2263463 27.686718 6
CGTTC 5217060 1.2237363 21.381777 1
TGGAT 10056640 1.2223094 15.908832 5
ACGAA 10266770 1.2140936 14.484176 2
TATGG 9972230 1.21205 5.318451 7
GATAT 14076045 1.2114484 35.02802 4
TTACG 8521980 1.2108314 20.848816 4
CCATC 4828755 1.2079523 7.084086 6
CCACA 5275210 1.2039042 5.792734 2
GGTCA 6544305 1.1979762 7.7750726 7
TCACA 7882110 1.194368 7.733979 9
CACCT 4766490 1.1923761 17.465052 4
CCTAA 7825670 1.1858157 52.04774 3
CTGTT 7613035 1.1856687 14.029698 8
ATCGA 9136915 1.1843506 15.082646 1
CTAGG 6449470 1.1806159 12.288622 1
ATCGT 8295465 1.1786473 5.353427 1
TTTGC 7562380 1.1777797 11.215737 7
CTCAG 5489185 1.1746472 5.134674 8
CGGTG 4539375 1.1735042 15.648443 6
TTCTG 7534915 1.1735022 19.678135 2
TGTTC 7511170 1.1698041 16.078983 9
CATTG 8212485 1.1668574 7.172412 6
CTGTG 5785045 1.1607903 19.627453 2
CGTGG 4488010 1.1602253 9.952709 7
ATCCA 7651130 1.1593678 20.980482 1
GCTAC 5410330 1.1577728 20.662718 2
TAAAA 20764205 1.1544452 7.661109 9
CGATT 8098235 1.1506243 62.287582 3
TGACA 8872105 1.1500255 5.6154666 9
TTATG 12163105 1.147443 18.792883 6
AGTCT 7998575 1.1364641 7.4218082 7
TGGCA 6195365 1.1341006 10.264823 9
ATCCT 6810930 1.1312653 6.995322 2
AGGTA 10151305 1.1256092 8.465886 2
TGCCG 3716625 1.1231875 6.9412613 9
TCTTT 9248190 1.1179459 11.180813 9
ACACT 7372555 1.1171558 8.742964 3
ATTCA 11046125 1.1113459 8.924756 7
CACTC 4410160 1.1032373 14.434238 4
TGGAC 6020495 1.1020894 5.2232046 9
CTACC 4380405 1.0957936 24.149311 3
TCATG 7696910 1.0936027 6.299928 9
CGTGC 3584330 1.083207 9.979497 7
CGCCT 3065465 1.0829661 91.526306 1
GATCG 5905430 1.081026 6.8869605 145-146
TATTT 14690575 1.0756824 61.83156 5
ACGTT 7568990 1.0754273 6.1418185 3
GGACA 6433345 1.0743829 23.727648 2
ACGTC 5003770 1.0707718 17.163218 6
TTTCT 8855340 1.0704571 14.407404 7
AATTC 10569335 1.0633762 10.848788 1
GTGGA 6763465 1.0591047 11.641441 8
TCGAA 8132200 1.0541168 15.100935 2
TTTGT 10181725 1.0528597 19.692286 7
AACGC 5392970 1.0528469 5.2810144 1
GACAA 8866025 1.0484489 8.305016 3
ACTCT 6310090 1.0480782 18.929037 5
GTGGC 4047005 1.0462182 10.935261 8
CTGAC 4875525 1.0433283 6.015665 1
CACAC 4560785 1.0408586 9.668207 3
TCCAC 4156695 1.0398308 6.343984 1
GATTA 12077905 1.0394793 37.728386 4
TACTG 7306680 1.0381575 6.085894 4
GATAA 13221360 1.0380971 8.392021 2
GTATC 7231435 1.0274664 5.7033668 8
CTATT 9306215 1.0263009 28.54347 4
CGTAT 7216060 1.0252819 43.816074 3
ATGGA 9201560 1.0202984 6.652415 4
ACCAT 6730920 1.0199295 16.596336 5
GACTC 4763310 1.019315 12.466251 4
TGTAG 8367580 1.0170165 6.5864606 4
CAACG 5208520 1.0168375 16.252111 1
ATTAT 15213890 1.0163027 29.122663 5
TGTCA 7145225 1.0152173 16.963707 9
ACCTG 4742605 1.0148844 22.357193 6
TCTGC 4323125 1.014051 29.658123 3
ACTGT 7067885 1.0042287 16.21973 1
ATCTC 6035070 1.0023984 6.013485 8
ATGTC 7007995 0.9957192 15.76935 4
CCATT 5988875 0.99472576 18.845877 5
TCAGG 5426060 0.9932743 10.198069 1
ATTCT 8964715 0.98863995 11.5849695 7
GCTTA 6956555 0.98841053 8.512645 6
GGATG 6253650 0.9792719 11.733442 6
TAGCA 7516115 0.9742583 6.3544087 9
TTAAA 15940620 0.9714616 6.3729568 8
GTAGT 7949895 0.96625024 10.321724 5
TATCT 8731355 0.9629047 7.7697787 7
GATGT 7896725 0.95978767 20.86356 3
TAACG 7332045 0.9503988 13.876461 4
GGATA 8557840 0.9489208 11.828798 1
GGCGT 3663590 0.94709915 79.57454 1
TCACC 3784190 0.9466457 17.46288 3
GTGTC 4689865 0.9410385 17.2587 8
TACCA 6204630 0.9401812 14.630664 4
GAATT 10915120 0.93940485 12.799557 2
TCGAC 4382465 0.93781686 17.716274 1
GGACT 5115200 0.93636954 10.208797 4
TGTTT 9031655 0.93393445 21.093708 9
GTCTG 4646520 0.93234116 19.387617 6
CATCT 5607930 0.93145245 9.304504 5
GTCAG 5080510 0.9300193 6.8989315 8
GTACT 6539405 0.92914045 6.897312 5
TCTTA 8416200 0.9281489 7.9415708 9
CCTAT 5559730 0.92344654 43.024677 3
TCTCT 5068530 0.9227892 12.393683 7
GCAAG 5520485 0.92193335 7.6505966 3
CAGGA 5520155 0.92187816 21.26014 2
GTTGT 6911300 0.92076814 7.796252 7
GAGGA 6409595 0.91566765 5.383786 4
TTCCG 3897225 0.9141501 25.303564 3
ACCTA 6004055 0.90978837 8.532133 6
ATTCC 5467385 0.90810865 17.902615 2
TCAGA 6997915 0.9070879 5.4421477 9
TGTGC 4513375 0.90562505 7.01277 7
TGTTA 9589850 0.9046872 8.861307 9
GTTCG 4499730 0.90288717 19.86337 4
ATGAG 8108120 0.8990544 23.808426 4
TGAGT 7396840 0.8990305 32.242153 5
ATTTC 8146905 0.89845085 24.680248 6
CCTCA 3576515 0.8946941 5.37854 7
ACGTA 6898415 0.89419055 9.704463 6
TATGC 6287690 0.89337593 5.495398 7
GACAC 4570990 0.89237505 11.267019 2
GCATG 4866575 0.89085716 10.889579 2
GTTCC 3777575 0.88608426 21.376345 2
CTCTA 5318780 0.88342583 8.225804 3
GTCAC 4071300 0.87122977 6.317277 8
TGTGA 7155900 0.86974597 11.896645 3
AGTCA 6686125 0.8666729 6.3258038 7
AGGAC 5188465 0.86648506 23.718462 1
GTGTG 5035535 0.86432624 13.244021 6
AGCGT 4708385 0.8618994 5.0770087 145-146
AGATC 6645965 0.8614673 5.368235 145-146
CGTCT 3646250 0.85528034 11.292361 7
TATTC 7729295 0.85239625 11.973638 6
CCTGG 2800670 0.84638005 6.1777105 7
GCATC 3945450 0.8442988 5.939334 2
AGACT 6471470 0.8388488 7.5538454 3
CTACT 5047115 0.83830345 5.1506214 1
CGACT 3896625 0.8338506 35.171818 2
TGTCG 4124625 0.827621 12.910995 3
TTATT 11283960 0.8262411 6.127512 8
ATGTT 8733860 0.82393485 9.114062 8
ACCTC 3286080 0.82203954 14.99117 6
TTGTG 6169985 0.8220054 10.434075 8
GACAT 6325955 0.8199867 16.648413 1
AGTGG 5232415 0.8193545 7.6420717 7
CTGTA 5757270 0.81801206 7.6960845 3
TCTGG 4074730 0.8176094 9.49767 7
CGGTC 2701050 0.81627434 9.776585 6
TCGCA 3793040 0.811684 10.02456 9
GGTCT 4027050 0.8080422 10.836665 7
TGGCG 3115265 0.80534786 6.5873804 9
CTGCC 2248485 0.79434365 6.031375 8
ACTCG 3711595 0.79425544 6.6227593 3
AGTGT 6468945 0.7862517 10.413269 7
TGTCC 3343015 0.78415203 15.323102 9
ATATT 11726925 0.78337 26.824823 5
ATTGT 8299795 0.78298616 13.739488 7
TACGA 5974685 0.7744542 15.866326 1
AGTGC 4214545 0.77149886 6.9153543 7
CCTCT 2803990 0.76887083 7.2050076 7
CCTGC 2172820 0.76761276 6.1970105 7
CGATA 5919410 0.7672894 52.764378 3
TGGGA 4873905 0.76321465 5.9597964 9
AACGA 6438215 0.761349 6.3157144 2
GCCAT 3535985 0.75667614 5.7916245 4
ACAGG 4516520 0.7542689 11.954509 1
ATTAA 12351410 0.7527261 5.5290847 7
ATTAC 7459310 0.75047797 14.763096 3
TCCTA 4500980 0.74759287 6.9925585 3
TACTC 4476920 0.7435966 5.137522 2
GTGCA 4049980 0.7413743 7.7549405 8
CTAAT 7259490 0.73037404 34.563496 4
CTGTC 3113030 0.7302057 11.762471 8
GTCGC 2394475 0.72362536 5.2484193 8
GAGTG 4593610 0.7193227 20.654781 6
GGATT 5918035 0.7192929 6.5603433 5
GGTTA 5911690 0.71852154 5.2534623 6
TGGAG 4580825 0.7173208 6.2475786 9
TCTGT 4603480 0.7169548 17.259918 7
GTGTA 5890470 0.71594244 6.749766 8
GGAAT 6448370 0.7150159 16.465809 1
TTAGC 4992790 0.70939225 5.3432508 8
AGTGA 6370235 0.70635206 6.3266096 7
TAGAA 8971135 0.7043837 6.0882235 9
GATCC 3257065 0.6969891 9.013046 1
CCGGT 2249190 0.6797193 32.29692 5
ACGAC 3479785 0.6793438 15.955763 1
GATCT 4768405 0.6775108 7.5292363 5
TGTGG 3942225 0.67666465 11.749128 9
TGTGT 5049740 0.67275906 19.789787 5
GTCTC 2862145 0.67135715 12.960324 6
TCGAT 4720065 0.6706425 5.4571543 5
TATTA 9999050 0.6679463 6.1523843 6
ACGAT 5151760 0.6677846 6.9227557 3
TAGGT 5475010 0.6654464 7.534281 1
AGTAA 8398655 0.65943444 5.020942 7
AGAGT 5934550 0.6580419 5.015205 5
TGAGA 5918235 0.65623295 8.602033 1
AGAGG 4585975 0.6551473 5.3856463 3
TAGTG 5373740 0.65313774 9.522786 6
AGACA 5521720 0.65296924 6.830452 1
TTTAT 8857720 0.64858544 5.202814 7
GCGTA 3542095 0.64840275 56.413284 2
CTGGA 3511565 0.64281404 7.846859 8
CGCCA 1989255 0.6411306 8.720488 3
ATCTG 4489165 0.6378355 6.6882124 6
ATTCG 4482670 0.63691276 5.0616755 7
CACAT 4198280 0.6361611 6.382051 5
TACGT 4359695 0.61943996 31.304518 5
CAGAG 3700920 0.61806184 5.51912 4
CATGT 4332665 0.6155995 12.99873 5
ACGCC 1896600 0.6112682 8.719087 2
AGCGC 2192995 0.6046147 10.162337 4
GTCAT 4229455 0.60093504 7.3796606 3
ATAAC 6515410 0.5980239 9.827748 3
CCTGT 2548090 0.59769106 12.707618 2
GTCGA 3236245 0.592415 7.2360106 4
TTGTA 6260750 0.5906267 6.7632985 8
TAATT 8808525 0.58841807 22.638428 5
GCGCT 1932440 0.5839955 10.906821 5
CTGAG 3176895 0.58155054 5.4425373 1
ATTTA 8662005 0.5786304 12.14208 6
CTCTC 2069515 0.5674734 9.056057 8
CTAGT 3909290 0.5554449 12.861177 5
AGCGG 2334825 0.5506561 6.5103292 4
GTCTA 3867390 0.54949164 7.3136616 6
TTCGC 2322565 0.544791 11.949499 1
GAGAC 3216460 0.5371559 9.734342 2
CGATC 2452665 0.5248532 11.4298115 4
AGCAT 4041200 0.52383083 11.291798 1
TTTCG 3231945 0.5033493 5.1813564 7
TATGT 5295740 0.49958956 10.221735 7
GGATC 2711700 0.49639374 8.065266 6
GAGTC 2688720 0.49218705 13.330852 6
TCTCG 2097425 0.49198112 7.1032724 9
GTGAG 3122995 0.4890362 8.429053 4
TGTAA 5475185 0.47121927 8.322978 9
TCGCC 1331160 0.47027153 18.010029 2
CGTAG 2547130 0.46626815 5.7654448 2
TCTAG 3249110 0.46164438 7.0388713 4
TAGTC 3079210 0.43750438 6.0814724 6
TTATA 6484575 0.43317592 6.084815 6
GAGTA 3802485 0.4216317 9.229575 6
TCCGG 1360330 0.41110027 32.60173 4
TCGCG 1345250 0.406543 7.7662525 2
ATGTG 3291685 0.4000796 7.3802977 4
TAGCG 2180580 0.39916882 6.7493978 3
CGCGT 1200350 0.36275333 7.768877 3
GTACG 1946655 0.35634744 13.829518 4