This README.txt file was generated on 20200824 by MAK, Wing Shum Winston.


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GENERAL INFORMATION
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1. Title of Dataset:Study of FPMINT derivative acting on Human Equilibrative Nucleoside Transporters


2. Author Information


First Author Contact Information
    Name:MAK, Wing Shum Winston
    Faculty:Medicine
    Email:makwsw@hku.hk


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DATA & FILE OVERVIEW
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Directory of Files:
   A. Filename:1. MTT assay      
      Short description:Effect of FPMINT derivative on the viability of PK15NTD/ENT1 and PK15NTD/ENT2 cells.    


        
   B. Filename:2. Nucleoside uptake assay        
      Short description:Effect of FPMINT derivative on [3H]adenosine and [3H]uridine uptake in PK15NTD/ENT1 and PK15NTD/ENT2 cells.       


        
   C. Filename:3. Western Blot        
      Short description:Effect of FPMINT derivative on the protein expression level of ENT1 and ENT2.


        
   D. Filename:4. Concentration Dependence and Kinetics       
      Short description:Inhibition of [3H] Uridine Uptake in PK15NTD/ENT1 and PK15NTD/ENT2 cells by FPMINT derivative, and Kinetic analysis of ENT1-and ENT2-mediated uridine uptake in
                        the presence of FPMINT derivative.


        
   E. Filename:5. Reversibility       
      Short description:Reversibility of inhibitory effects of FPMINT derivative on ENT1 and ENT2.

Additional Notes on File Relationships, Context, or Content 
Not applicable.            


File Naming Convention:
They are named as the main items to be tested in the experiements.


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DATA DESCRIPTION FOR: [FILENAME]
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1. Number of variables:5


2. Number of cases/rows: 5


3. Missing data codes:Not applicable.



4. Variable List:


   A. Name:Cell viability      
      Description:Effect of FPMINT derivative on the viability of PK15NTD/ENT1 and PK15NTD/ENT2 cells.    


        
   B. Name:Dosage-activity relationship between FPMINT and nucleoside uptake        
      Description:Effect of FPMINT derivative on [3H]adenosine and [3H]uridine uptake in PK15NTD/ENT1 and PK15NTD/ENT2 cells.       


        
   C. Name:Protein expression levels of ENT1 and ENT2       
      Description:Effect of FPMINT derivative on the protein expression level of ENT1 and ENT2.


        
   D. Name:Mechanism of inhibition from FPMINT derivative     
      Description:Inhibition of [3H] Uridine Uptake in PK15NTD/ENT1 and PK15NTD/ENT2 cells by FPMINT derivative, and Kinetic analysis of ENT1-and ENT2-mediated uridine uptake in
                  the presence of FPMINT derivative


        
   E. Name:Reversibility       
      Description:Reversibility of inhibitory effects of FPMINT derivative on ENT1 and ENT2.


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METHODOLOGICAL INFORMATION
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1. Software-specific information:

 
   A. Name:GraphPad Prism
      Version:5.01
      System Requirements:Window
      Open Source? (Y/N):N

   B. Name:Microsoft Excel
      Version:Microsoft 365
      System Requirements:Window
      Open Source? (Y/N):Y

Additional Notes:Not applicable.

2. Equipment-specific information:


   A.Manufacturer:PerkinElmer
     Model:LS6500

Additional Notes:Not applicable.