This README.txt file was generated on <20210429> by <Chaoran Shi>


-------------------
GENERAL INFORMATION
-------------------

1. Title of Dataset:
Supporting data for "Characterization of the Cancer Stemness Effect of gene MAEL on HCC"


2. Author Information
<create a new entry for each additional author>

First Author Contact Information
    Name: Chaoran Shi
    Faculty: Medicine
    Email: u3005126@connect.hku.hk



Corresponding Author Contact Information
    Name: Xin-yuan GUAN
    Faculty: Medicine
    Email:xyguan@hku.hk

---------------------
DATA & FILE OVERVIEW
---------------------


Directory of Files:
   A. Filename: Support data_Folder1_CD133 flow     
      Short description: include flow cytometry results of CD133 staining in 8024, Huh7 and MIHA cells.The FSC file needs to be processed in FlowJo Software.       

      
   B. Filename: Support data_Folder2_Sorafenib apotopsis     
      Short description: flow cytometry results of Annexin/PI staining in 8024, Huh7 cells after exposed in Sorafenib.The FSC file needs to be processed in FlowJo Software.       


        
   C. Filename: Support data_Folder3_Clinical data       
      Short description: Include txt file showing MAEL and PTGS2 expression in HCC tumor and normal
 Tissues. Include sav file of clinical parameters of HCC patients in TCGA and HKU cohort, which can be open using SPSS software and XL sheet showing the qPCR result of HBx and TXNIP expression pattern in clinical samples. Include XL sheets showing gene expression levels and clinical characteristics in HCC samples from TCGA dataset.


   D. Filename: Support data_Folder4_Functional assay       
      Short description: Include images in jpg format showing foci formation, colony formation in soft agar, migration and invasion assay. Include XL sheets showing each experiment of events count. Include pzf format showing the statistical analysis.


   E. Filename: Support data_Folder5_IF    
      Short description: Include images in tiff format showing IF staining against different antibodies in 8024 and Huh7 cells.	


   F. Filename: Support data_Folder6_In vivo Sorafenib treatment
      Short description: Include images in jpg format showing knockdown MAEL with Sorafenib treatment in 8024 cell lines formatted mice xenograft.XL sheets showing tumor volume in nude mice, and pzf format showing the statistical analysis using graphPad software.


   G. Filename: Support data_Folder7_IL-8 ELISA      
      Short description: Include XL sheets showing the IL-8 gene expression levels in 8024, MIHA and Huh7 cells.


   H. Filename: Support data_Folder8_RNA seq     
      Short description: Include XL sheets of RNA-seq showing gene expression of key enrichment pathway in stemness regulating. Include png, TIFF or pdf of gene enriched signaling pathways. 

   I. Filename: Support data_Folder9_Tumor formation in nude mice
      Short description: Include images in jpg format showing tumor size  XL sheets showing tumor volume in nude mice, and pzf format showing the statistical analysis using graphPad software.

	

Additional Notes on File Relationships, Context, or Content 
(for example, if a user wants to reuse and/or cite your data, 
what information would you want them to know?): N/A             


File Naming Convention:N/A




-----------------------------------------
DATA DESCRIPTION FOR: [N/A]
-----------------------------------------
<create sections for each dataset included>


1. Number of variables:N/A


2. Number of cases/rows: N/A


3. Missing data codes:N/A
        Code/symbol        Definition
        Code/symbol        Definition


4. Variable List: N/A




--------------------------
METHODOLOGICAL INFORMATION
--------------------------

#
# Software: If specialized software(s) generated your data or
# are necessary to interpret it, please provide for each (if
# applicable): software name, version, system requirements,
# and developer. 
#If you developed the software, please provide (if applicable): 
#A copy of the software’s binary executable compatible with the system requirements described above. 
#A source snapshot or distribution if the source code is not stored in a publicly available online repository.
#All software source components, including pointers to source(s) for third-party components (if any)

1. Software-specific information:
<create a new entry for each qualifying software program>

Name: FlowJo
Version: 7.6.1
System Requirements: Windows
Open Source? (Y/N): Y

(if available and applicable)
Executable URL:N/A
Source Repository URL: N/A
Developer: Becton, Dickinson and Company
Product URL:N/A
Software source components:N/A

Name: SPSS Statistics 
Version: 24
System Requirements: Windows 
Open Source? (Y/N): Y

(if available and applicable)
Executable URL:N/A
Source Repository URL: N/A
Developer: IBM
Product URL:N/A
Software source components:N/A

Additional Notes(such as, will this software not run on 
certain operating systems?): N/A

Name: GraphPad Prism
Version: 5.0
System Requirements: Windows or Mac
Open Source? (Y/N): Y

(if available and applicable)
Executable URL:N/A
Source Repository URL: N/A
Developer: GraphPad
Product URL:N/A
Software source components:N/A

Additional Notes(such as, will this software not run on 
certain operating systems?): N/A


2. Equipment-specific information:
<create a new entry for each qualifying piece of equipment>

Manufacturer:N/A
Model:N/A

(if applicable)
Embedded Software / Firmware Name:N/A
Embedded Software / Firmware Version:N/A
Additional Notes:N/A