This README.txt file was generated on 2021/08/25 by Qiu Yinxia # # General instructions for completing README: # For sections that are non-applicable, mark as N/A (do not delete any sections). # Please delete all commented sections after you completed this README . # ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset:Qiu Yinxia_Dataset # # Authors: Include contact information for at least the # first author and corresponding author (if not the same), # specifically email address and faculty. # Contact information for all authors is preferred. # 2. Author Information First Author Contact Information Name:Qiu Yinxia Faculty:Medicine Email:u3006803@connect.hku.hk Corresponding Author Contact Information Name:Qiu Yinxia Faculty:Medicine Email:u3006803@connect.hku.hk Author Contact Information (if applicable) Name: Faculty: Email: --------------------- DATA & FILE OVERVIEW --------------------- # # Directory of Files in Dataset: List and define the different # files included in the dataset. This serves as its table of # contents. # Directory of Files: A. Filename:1 western blotting Short description:The first file contains the western blotting images obtained from this study. For each folder may contain several subfolders. There were four cell lines tested in this study: KE-37, CCRF-CEM, LOUCY and MOLT-16. B. Filename:2 q-PCR Short description:The second file contains the RT-qPCR results obtained from this study. For each folder may contain several subfolders. There were four cell lines tested in this study: KE-37, CCRF-CEM, LOUCY and MOLT-16. C. Filename:3 MTT Short description:The third file contains the MTT results obtained from this study.For each folder may contain several subfolders. There were four cell lines tested in this study: KE-37, CCRF-CEM, LOUCY and MOLT-16. D. Filename:4 apoptosis Short description:The forth file contains the apoptosis results obtained from this study.For each folder may contain several subfolders. There were four cell lines tested in this study: KE-37, CCRF-CEM, LOUCY and MOLT-16. Additional Notes on File Relationships, Context, or Content (for example, if a user wants to reuse and/or cite your data, what information would you want them to know?): # # File Naming Convention: Define your File Naming Convention # (FNC), the framework used for naming your files systematically # to describe what they contain, which could be combined with the # Directory of Files. # File Naming Convention:numerical order(space) method name Example: 1 western blotting or (for subfolders): Cell line name (space) date conducting the experiment Example:CEM 2020.07.31 # # Data Description: A data description, dictionary, or codebook # defines the variables and abbreviations used in a dataset. This # information can be included in the README file, in a separate # file, or as part of the data file. If it is in a separate file # or in the data file, explain where this information is located # and ensure that it is accessible without specialized software. # (We recommend using plain text files or tabular plain text CSV # files exported from spreadsheet software.) # ----------------------------------------- DATA DESCRIPTION FOR: [1 western blotting] ----------------------------------------- 1. Number of variables:2 2. Number of cases/rows: 56 3. Missing data codes: Code/symbol Definition Code/symbol Definition 4. Variable List A. Name: cell line name Description: there are four cell lines tested, namely KE-37, CCRF-CEM, LOUCY and MOLT-16. B. Name: treatment regimens Description: Ctrl, A8, NH4C1, A8+NH4C1, Rap200nM, A8+ Rap200nM or Ctrl, A8, B1, A8+ B1, B2.5, A8+ B2.5, B5, A8+ B5 ----------------------------------------- DATA DESCRIPTION FOR: [2 RT-qPCR] ----------------------------------------- 1. Number of variables:3 2. Number of cases/rows: 216 3. Missing data codes: Code/symbol Definition Code/symbol Definition 4. Variable List A. Name: cell line name Description: there are four cell lines tested, namely KE-37, CCRF-CEM, LOUCY and MOLT-16. B. Name: treatment regimens Description: Ctrl, A8 or A2 C. Name: gene name Description: gapdh, Beclin1, LC3B, ATG5, ATG7, ATG4B, ATG12, ULK1, SQSTM1. ----------------------------------------- DATA DESCRIPTION FOR: [3 MTT] ----------------------------------------- 1. Number of variables:2 2. Number of cases/rows: 1008 3. Missing data codes: Code/symbol Definition Code/symbol Definition 4. Variable List A. Name: cell line name Description: there are four cell lines tested, namely KE-37, CCRF-CEM, LOUCY and MOLT-16. B. Name: treatment regimens Description: Ctrl, A8/A2, NH4Cl, A8+NH4Cl, Rap200nM, A8+Rap200nM, or ctrl, A8/A2, U, U+A, SP, SP+A, SB, SB+A ----------------------------------------- DATA DESCRIPTION FOR: [4 apoptosis] ----------------------------------------- 1. Number of variables:2 2. Number of cases/rows: 432 3. Missing data codes: Code/symbol Definition Code/symbol Definition 4. Variable List A. Name: cell line name Description: there are four cell lines tested, namely KE-37, CCRF-CEM, LOUCY and MOLT-16. B. Name: treatment regimens Description: ctrl,A （2/8 μM）, NH4Cl, N+A,B5, B5+A , U, U+A, SP, SP+A, SB, SB+A. -------------------------- METHODOLOGICAL INFORMATION -------------------------- # # Software: If specialized software(s) generated your data or # are necessary to interpret it, please provide for each (if # applicable): software name, version, system requirements, # and developer. #If you developed the software, please provide (if applicable): #A copy of the software’s binary executable compatible with the system requirements described above. #A source snapshot or distribution if the source code is not stored in a publicly available online repository. #All software source components, including pointers to source(s) for third-party components (if any) 1. Software-specific information: Flowjo Name:Flowjo Version:10.4.0 System Requirements:V10.8.0 or above Open Source? (Y/N): Y (if available and applicable) Executable URL:N/A Source Repository URL:N/A Developer:FlowJo, LLC Product URL:N/A Software source components:N/A Additional Notes(such as, will this software not run on certain operating systems?):N/A # # Equipment: If specialized equipment generated your data, # please provide for each (if applicable): equipment name, # manufacturer, model, and calibration information. Be sure # to include specialized file format information in the data # dictionary. # 2. Equipment-specific information:Alliance Q9 Advanced Chemiluminescence Imager Manufacturer:Alliance Model:Q9 (if applicable) Embedded Software / Firmware Name:N/A Embedded Software / Firmware Version:N/A Additional Notes:N/A Equipment-specific information:Roche LightCycler 480 II instrument Manufacturer:Roche Model:480 II (if applicable) Embedded Software / Firmware Name:N/A Embedded Software / Firmware Version:N/A Additional Notes:N/A Equipment-specific information:multifunctional plate reader Manufacturer: Tecan Model:Infinite® F200 (if applicable) Embedded Software / Firmware Name:N/A Embedded Software / Firmware Version:N/A Additional Notes:N/A Equipment-specific information:BD LSRFortessa™ flow cytometer Manufacturer:BD biosciences Model:BD LSRFortessa™ (if applicable) Embedded Software / Firmware Name:N/A Embedded Software / Firmware Version:N/A Additional Notes:N/A