This README.txt file was generated on <20211209> by <ZHOU Bo>

#
# General instructions for completing README: 
# For sections that are non-applicable, mark as N/A (do not delete any sections). 
# Please delete all commented sections after you completed this README . 
#

-------------------
GENERAL INFORMATION
-------------------

1. Title of Dataset:
single cell transcriptome profiling of neutrophils


2. Author Information
<create a new entry for each additional author>

First Author Contact Information
    Name: ZHOU Bo
    Faculty: Faulty of Medicine
    Email: u3005199@connect.hku.hk



Corresponding Author Contact Information
    Name: You-qiang Song
    Faculty: Faculty of Medicine
    Email: songy@hku.hk



---------------------
DATA & FILE OVERVIEW
---------------------

#
# Directory of Files in Dataset: List and define the different 
# files included in the dataset. This serves as its table of 
# contents. 
#

Directory of Files:
   A. Filename: Bm-1Y/       
      Short description: Single cell sequencing data of mouse bone marrow cells        


        
   B. Filename: Bm-2Y/       
      Short description: Single cell sequencing data of mouse bone marrow cells       


        
   C. Filename: Bm-3Y/       
      Short description: Single cell sequencing data of mouse bone marrow cells



   D. Filename: Bm-4Y/       
      Short description: Single cell sequencing data of mouse bone marrow cells



   E. Filename: Bl-1Y/       
      Short description: Single cell sequencing data of mouse blood cells



   F. Filename: BM1Y/       
      Short description: Single cell sequencing data of mouse bone marrow cells



   G. Filename: BM2Y/       
      Short description: Single cell sequencing data of mouse bone marrow cells



   H. Filename: BM1O/       
      Short description: Single cell sequencing data of mouse bone marrow cells



   I. Filename: BM2O/      
      Short description: Single cell sequencing data of mouse bone marrow cells



   J. Filename: BL1Y/       
      Short description: Single cell sequencing data of mouse blood cells



   K. Filename: BL2Y/       
      Short description: Single cell sequencing data of mouse blood cells



   L. Filename: BL1O/       
      Short description: Single cell sequencing data of mouse blood cells


Additional Notes on File Relationships, Context, or Content 
(for example, if a user wants to reuse and/or cite your data, 
what information would you want them to know?):              

In each directory, there are three files, which are barcodes.tsv.gz, 
features.tsv.gz, matrix.mtx.gz. These three files contain information 
of cellular barcodes, molecule identifier of mRNA, and gene expression counts.


#
# File Naming Convention: Define your File Naming Convention 
# (FNC), the framework used for naming your files systematically 
# to describe what they contain, which could be combined with the
# Directory of Files. 
#

File Naming Convention:
scRNA

#
# Data Description: A data description, dictionary, or codebook
# defines the variables and abbreviations used in a dataset. This
# information can be included in the README file, in a separate 
# file, or as part of the data file. If it is in a separate file
# or in the data file, explain where this information is located
# and ensure that it is accessible without specialized software.
# (We recommend using plain text files or tabular plain text CSV
# files exported from spreadsheet software.) 
#

-----------------------------------------
DATA DESCRIPTION FOR: [FILENAME]
-----------------------------------------
<create sections for each dataset included>


1. Number of variables:


2. Number of cases/rows: 


3. Missing data codes:
        Code/symbol        Definition
        Code/symbol        Definition


4. Variable List

#
# Example. Name: Gender 
#     Description: Gender of respondent
#         1 = Male
#         2 = Female
#         3 = Transgender
#	      4 = Nonbinary
#		  5 = Other gender not listed 
#		  6 = Prefer not to answer
#

    A. Name: <variable name>
       Description: <description of the variable>
                    Value labels if appropriate


    B. Name: <variable name>
       Description: <description of the variable>
                    Value labels if appropriate

--------------------------
METHODOLOGICAL INFORMATION
--------------------------

#
# Software: If specialized software(s) generated your data or
# are necessary to interpret it, please provide for each (if
# applicable): software name, version, system requirements,
# and developer. 
#If you developed the software, please provide (if applicable): 
#A copy of the software’s binary executable compatible with the system requirements described above. 
#A source snapshot or distribution if the source code is not stored in a publicly available online repository.
#All software source components, including pointers to source(s) for third-party components (if any)

1. Software-specific information:
<create a new entry for each qualifying software program>

Name: CellRanger
Version: 3.0.1
System Requirements: 8-core Intel or AMD processor (16 cores recommended); 64GB RAM (128GB recommended); 1TB free disk space; 64-bit CentOS/RedHat 6.0 or Ubuntu 12.04
Open Source? (Y/N): Y

(if available and applicable)
Executable URL:
Source Repository URL:
Developer:
Product URL:
Software source components:


Additional Notes(such as, will this software not run on 
certain operating systems?):


#
# Equipment: If specialized equipment generated your data,
# please provide for each (if applicable): equipment name,
# manufacturer, model, and calibration information. Be sure
# to include specialized file format information in the data
# dictionary.
#

2. Equipment-specific information:
<create a new entry for each qualifying piece of equipment>

Manufacturer:
Model:

(if applicable)
Embedded Software / Firmware Name:
Embedded Software / Firmware Version:
Additional Notes: