1. Title of Dataset:
Supporting data for Neuroprotective role of α-Melanocyte-stimulating hormone on ischemia/reperfusion- induced brain and retinal damage in type 1 diabetic mouse

Authors:
Rajesh Kumar Goit, Department of Ophthalmology, rgoit@hku.hk
Amy CY Lo, Department of Ophthalmology, amylo@hku.hk

2. Author Information
First Author Contact Information
      Name: Rajesh Kumar Goit
      Faculty: Faculty of Medicine
      Email: rgoit@hku.hk

Corresponding Author Contact Information
      Name: Amy CY Lo
      Faculty: Faculty of Medicine
      Email: amylo@hku.hk

Author Contact Information
      Name: Wai-Ching Lam
      Faculty: Faculty of Medicine
      Email: waichlam@hku.hk


DATA & FILE OVERVIEW
Directory of Files
Brain:
Neuropeptide α- Melanocyte-stimulating hormone promotes neurological recovery and repairs cerebral ischemia/reperfusion injury in type 1 diabetes.
	Immunohistochemistry (IHC)
		Glial fibrillary acidic protein (GFAP)
		Ionized calcium-binding adapter molecule 1 (IBA1)
		Nitrotyrosine (NT)
		Poly (ADP-ribose) (PAR) 
	Middle Cerebral Artery Occlusion (MCAO)
		Brain sections after triphenyl tetrazolium chloride (TTC) Staining
	Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)
	Western Blot (WB)
		Matrix metallopeptidase 9 (MMP-9)
		Cyclooxygenase-2 (COX-2)
		Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)
		Protein kinase B (PKB), also known as Akt
		Heme Oxygenase-2 (HO-2)
		Heme Oxygenase-1 (HO-1)
		B-cell lymphoma 2 (Bcl-2)
		Beta-actin
Retina:
Anti-inflammatory alpha-Melanocyte-stimulating hormone protects retina after ischemia/reperfusion injury in type 1 diabetes.
	Electroretinography (ERG)
		Amplitudes of a-wave, b-wave and oscillatory potentials
	Hematoxylin and eosin (H&E) stain
		Layers of retina
	Immunohistochemistry (IHC)
		Calretinin
		Glial fibrillary acidic protein (GFAP)
		Glutamine synthetase (GS)
   		Nitrotyrosine (NT)
		Poly (ADP-ribose) (PAR) 
		Protein kinase C alpha (PKCα)
	Western blot
		Interleukin 10 (IL-10)
		B-cell lymphoma 2 (Bcl-2)
		Glyceraldehyde 3-phosphate dehydrogenase (GAPDH)